Content changes of assimilative pigments in leaves after fertilizer Mg-Titanit application

Abstract

. The positive impact of Mg-Titanit on the content of the total chlorophyll was carried out by the increase of the amount of both assimilative pigments (chlorophyll a and chlorophyll b).Keywords: chlorophyll, fertilizer, titanium, winter wheat, winter rape impact of two doses of the fertilizer containing titanium, which were applied on two different crops (cereals and oil plants) during the growing season (in the first treatmentdouble application and in the second treatment -triple application) on total chlorophyll content in the leaves of winter wheat and winter rape. Materials and methodsThe dynamics of the content changes of the assimilative pigments after the application of the fertilizer Mg-Titanit (MGT) was studied in the field small-area trial carried out at the Haplic Chernozem (48° 42' N, 17° 70' E) during two years (2010 and 2011) with two crops (winter bread wheat and winter rape). The agrochemical parametres of the trial plots are given in the Table 1. They were determined by the following methods: N-NH 4 + -colorimertically by Nessler`s agent, N-NO 3 --colorimertically by the phenol 2.4 disulphonic acid, N an = N-NH 4 + + N-NO 3 -, Pcolorimertically (Mehlich III -Mehlich, 1984), K and Caflame photometry (Mehlich III -Mehlich, 1984), Mg -AAS (Mehlich III -Mehlich, 1984), C ox -oxidometrically (Ťjurin, 1966), N t -by distilling (Kjeldahl -Bremner, 1960), pH/ KCl -potentiometrically (1.0 mol.dm -3 KCl). The wheat and rape trials involved 5 variants. Var. 1: 0Ti -control, MGT unfertilized variant; var. 2: 2 × Ti 0.2 -spraying twice by MGT in the dose 0.2 l ha . The fertilizer Mg-Titanit contained 3 % magnesium, 4 % sulphur, and 8.5 g of titanium in 1 litre, where titanium was in the form of titanium ascorbate and sulphur and magnesium in the form of magnesium sulfate (MgSO 4 ). The fertilizer Mg-Titanit, which has been categorized as a biostimulator since 2013, was applied at two or three growth stages: at wheat in the phenophases BBCH 29, BBCH 32, BBCH 55; at rape in the phases BBCH 50 -52, BBCH 59, BBCH 66 -67. 14 to 21 days after the fertilizer application by spraying the samples of the youngest, fully developed sound leaves were analysed from both crops and the pigment contents were determined. The spectrophotometric method was used to determine the content of the assimilative pigments (chlorophyll a, chlorophyll b and carotenoids) in the acetonic extract. Extract was centrifuged 2 minutes at 2500 rpm. Absorbance (A) of the solution was measured by UV-VIS spectrophotometer (Jenway, UK), at 470 nm (carotenoids), 647 nm (chlorophyll b), and 663 nm (chlorophyll a), with correction for scattering at 750 nm; the measurements were done in three repetitions. The concentrations of chlorophyll a (Chl a), chlorophyll b (Chl b) and carotenoids (Car) in mg l -1 were determined by using the equations of Lichtenthaler (1987) Results and discussionThe pigment biosynthesis in plants is influenced by a whole range of external and internal factors. Apart from the genetic factors also the light, temperature, water ...

Effect of Tytanit on Selected Physiological Characteristics, Chemical Composition and Production of Festulolium braunii (K. Richt.) A. Camus

“…A. Camus. In addition, according to Radkowski [32] and Kováčik et al [33], Tytanit increased chlorophyll concentration in the leaves of Phleum pratense L., Triticum aestivum L. and Brassica napus var. napus.…”

Growth and Yield Stimulation of Winter Oilseed Rape (Brasssica Napus L.) by Mg-Titanit Fertiliser

“…Its effect on the formation of the abo-veground and underground phytomass was slightly positive, in particular, it had more positive impact on roots (cca 5.0% increase) than on the aboveground part (cca from 1.5-3.0% increase). The different conclusion was achieved by Kováčik et al (2014), the third spraying by Mg-Titanit, which was used in the growth phase BBCH 55, inhibited the formation of the aboveground wheat phytomass.…”

“…The first and partially also the second spraying (application in the growth phases BBCH 50-52 and BBCH 59, and measurement in phases BBCH 59 and BBCH 66-67) had more significant impact on the growth of chlorophyll b than the growth of chlorophyll a. Similarly, Kováčik et al (2014) monitored a higher increase of chlorophyll b content in the wheat leaves than chlorophyll b after several applications of Mg-Titanit. On the contrary, after the autumn application of the pig manure on maize and sunflower Kováčik (2014) T a b l e 8…”