SummaryThe uptake and metabolism of [3H]leucine, [U-14C]glucose, and [3H]palmitate were studied in rabbits aged -3, 1, 7, 28, and 90 days up to 1-1.5 years. Although lung composition did not change markedly, there were great differences in lung metabolism between the perinatal period (late fetal and newborn) and later stages of development.Leucine incorporation into lung protein was highest (3.1-3.4 nmollmg protein/hr) in late fetal and newborn rabbits and decreased rapidly thereafter. Palmitic acid incorporation decreased during the first week after birth from 150 nmo1/100 m d h r to 85 nn101/100 mg/hr at 7 days of age; it increased thereafter to 170 nmo1/100 mdhr at 4 weeks of age and remained at that level throughout the entire period studied. Glucose uptake and lactate production were higher in fetal lungs than in all other age groups. Lipid biosynthesis from glucose was 2-4 times higher in fetal lungs than at all other ages; furthermore, more than 60% of glucose carbon atoms channeled into lipid was incorporated into fatty acids, whereas at all other ages glucose was chiefly a precursor of lipid glycerol.
SpeculationOur data suggest that late fetal lung has a high metabolic activity that corresponds to the high synthetic demands of a rapidly growing and differentiating organ. Our study shows a higher glucose uptake in fetal and newborn lung than at later ages. Of special significance is the finding that lipid and, especially, fatty acid synthesis from glucose, is highest in the fetal lung. Since glucose is incorporated chiefly into phospholipids, the 7-8 times higher incorporation of glucose carbons into phospholipid fatty acid could have special significance for surfactant biosynthesis.Surfactant production is closely linked to lung maturation and is of prime importance to the ability of the premature o r term infant to survive (9, 13). It is for this reason that most of the interest in lung metabolism during the perinatal period has been focused on surfactant synthesis and secretion (13,14,20,28). With the exception of collagen (3) and glycosaminoglycan synthesis (18), very little is known on other aspects of lung metabolism during this period of rapid lung growth; in addition, there are only few systematic studies that extend throughout the developmental period into adulthood and old age. In the present study, we have investigated glucose metabolism and lipid and protein synthesis in rabbit lung from 3 days before birth to 2 years of age.
MATERIALS AND METHODS
ANIMALSNew Zealand white rabbits were obtained from the B and H Rabbitry, Rockville, MD. Newborn rabbits up to 3 weeks of age were on a diet of mother's milk only; between 3 and 8 weeks they were on a mixed diet of milk and solid food, and 9 after 8 weeks they received only solid food (nonmedicated Purina rabbit chow). Old rabbits were breeding females 1-1.5 years old. The animals were anesthetized with sodium Nembutal (Abbott Laboratories, Chicago, IL), 5 0 mg/kg iv for rabbits 3 months and older, or 25-50 mg/100 g ip for the younger rabbits. The ...