2018
DOI: 10.1111/lam.12883
| View full text |Cite
|
Sign up to set email alerts
|

Abstract: Significance and Impact of the Study: The number of opportunistic infections caused by Corynebacteria is increasing due to increase in number of immunocompromised patients. New Corynebacterium species and new human infections, caused by this group of bacteria, has been described recently. However, identification of Corynebacteria is still a challenge despite application of sophisticated laboratory methods. In the study we present possibilities and limitations of various commercial systems for identification of… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

0
34
0

Year Published

2018
2018
2023
2023

Publication Types

Select...
8

Relationship

0
8

Authors

Journals

citations
Cited by 54 publications
(34 citation statements)
references
References 60 publications
(65 reference statements)
0
34
0
Order By: Relevance
“…Through the development of more sophisticated molecular methods, pathogens can now be detected and identified directly from clinical samples. In MALDI-TOF MS, identification of microbes is through generation of characteristic peptide mass fingerprint spectra and comparing them to sequences of known organisms in the database 41. Due to the low cost, accuracy and speed, MALDI-TOF MS analysis is increasingly used routinely for Corynebacterium identification 42.…”
Section: Microbiologymentioning
confidence: 99%
See 1 more Smart Citation
“…Through the development of more sophisticated molecular methods, pathogens can now be detected and identified directly from clinical samples. In MALDI-TOF MS, identification of microbes is through generation of characteristic peptide mass fingerprint spectra and comparing them to sequences of known organisms in the database 41. Due to the low cost, accuracy and speed, MALDI-TOF MS analysis is increasingly used routinely for Corynebacterium identification 42.…”
Section: Microbiologymentioning
confidence: 99%
“…Several studies show that MALDI-TOF MS can accurately identify most Corynebacterium species to the genus (up to 99.5%) and species (up to 92.3%) levels 35 43 44. The gold standard for Corynebacterium species identification is through sequencing of rpoB gene and 16S rRNA gene 41. However, identification of Corynebacteria in formalin-fixed paraffin-embedded tissue can be technically challenging due to cross-linking and fragmentation of nucleic acids as well as low sensitivity of molecular methods for the detection of extremely rare organisms in tissue sections 16.…”
Section: Microbiologymentioning
confidence: 99%
“…The Corynebacterium genus currently numbers around 129 species and subspecies isolated from human, mammals, and environmental sources [1]. Many species are members of the normal skin and the upper respiratory tract microbiota and can produce infections, especially in immune-compromised or debilitated human patients [2].…”
Section: Introductionmentioning
confidence: 99%
“…To identify the species that belong to Corynebacterium genus, several types of methods are used: phenotypic, molecular, and mass spectrometry-based methods. Due to this variety of identifications possibilities, new species from the Corynebacterium genus have been isolated from clinical samples [1], such as the following that are presented below, the most frequent isolated species. Unfortunately studies about prevalence of the infections caused by these microorganisms, either in hospitalized patients or out-patients, are scarce.…”
Section: Introductionmentioning
confidence: 99%
“…This setting keeps the identification of Corynebacteria difficult and limited to the species included into appropriate databases. In addition, misidentification due to matching with closest patterns among the database entries can happen [9].…”
Section: Resultsmentioning
confidence: 99%