Contamination of pig carcass with Salmonella enterica serovar Typhimurium monophasic variant 1,4[5],12:i:- originates mainly in live animals

Marín, Clara; Chinillac, Ma Carmen; Cerdà‐Cuéllar, Marta; Montoro-Dasi, Laura; Sevilla‐Navarro, Sandra; Ayats, Teresa; Marco‐Jiménez, Francisco; Vega, Santiago

doi:10.1016/j.scitotenv.2019.134609

Cited by 17 publications

(15 citation statements)

References 27 publications

(18 reference statements)

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“…Salmonella 4,[5],12:i:- has increased significantly in human cases of Salmonellosis within the past two decades [ 10 ]. In Europe, Salmonella 4,[5],12:i:- was the third most common serotype of human Salmonellosis , accounting for 7.9% of foodborne disease outbreaks [ 33 ]. In recent years, this serotype has been increasing in China, and it has become one of the four most common serotypes causing human Salmonellosis [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Highly prevalent MDR, frequently carrying virulence genes and antimicrobial resistance genes in Salmonella enterica serovar 4,[5],12:i:- isolates from Guizhou Province, China

Long

Wang

et al. 2022

PLoS ONE

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Salmonella enterica serovar 4,[5],12:i:-, a monophasic variant of Salmonella Typhimurium lacking the phase 2 flagellin, is one of the common serotypes causing Salmonellosis worldwide. However, information on Salmonella serovar 4,[5],12:i:- from Guizhou Province has lacked so far. This study aimed to investigate the antimicrobial resistance, the presence of antimicrobial resistance genes and virulence genes, and characterize the MLST genotypes of Salmonella serovar 4,[5],12:i:- isolates from Guizhou province, China. We collected 363 non-typhoid Salmonella (NTS) isolates of Guizhou from 2013 to 2018. Biochemical identification, serogroups testing, and specific multiplex polymerase chain reaction (mPCR) assay were conducted to identify Salmonella 4,[5],12:i:- isolates. Isolates were determined the antimicrobial resistance by the micro broth dilution method, detected the presence of antimicrobial resistance genes and virulence genes by PCR, and examined the molecular genotyping by Multilocus sequence typing (MLST). Eighty-seven Salmonella 4,[5],12:i:- isolates were detected, accounting for 23.9% (87/363) of the total NTS isolates. All Salmonella 4,[5],12:i:- isolates showed highly resistant to sulfaoxazole (93.1%), streptomycin (90.8%), ampicillin (88.5%), tetracycline (86.2%) and doxycycline (86.2%). A high proportion (94.2%) of multi-drug resistance (MDR) isolates were found. Most (83.9%) Salmonella 4,[5],12:i:- isolates carried four antimicrobial resistance genes, especially blaTEM-1, strA-strB, sul2, and tetB genes. Salmonella 4,[5],12:i:- isolates showed a high rate of invA, sseL, mgtC, siiE, sopB, gipA, gtgB, sspH1, and sspH2 (72.4%~98.9%). On the contrary, none of the isolates were detected the spvC and pefA genes. MLST analysis revealed three sequence types (STs), and ST34 (97.7%) was the dominant sequence type. This study is the first report of Salmonella 4,[5],12:i:- in humans from Guizhou province, China. The data might be useful for rational antimicrobial usage against Salmonella 4,[5],12:i:- infections, risk management, and public health strategies in Guizhou.

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Section: Discussionmentioning

confidence: 99%

Highly prevalent MDR, frequently carrying virulence genes and antimicrobial resistance genes in Salmonella enterica serovar 4,[5],12:i:- isolates from Guizhou Province, China

Long

Wang

et al. 2022

PLoS ONE

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“…This study was carried out in swine slaughterhouses in the Valencia Region ( n = 8), Eastern Spain. The processing plants involved in this study slaughter 90% of the swine production in the Valencia Region [ 28 ]. During one year, 21 visits to the slaughterhouse were done to intensively sampling 21 different batches of swine.…”

Section: Methodsmentioning

confidence: 99%

“…Then, carcass samples were collected at two moments during processing: before and after cooling. To this end, a 100 cm 2 area of the ham, belly, rump, and jowl from each swine selected were swabbed—rubbing a sterile swab (bioMerieux, Madrid, Spain) 10 times vertically and horizontally [ 28 ]. Moreover, environmental samples were taken from two sites (knives, whips) and from the slaughtering staff (operators) by vigorous swabbing of the surfaces with swabs.…”

Section: Methodsmentioning

confidence: 99%

“…The implementation of efficient measures at critical points at the slaughterhouse level could therefore improve control of the pathogen and its AMR in the final product [ 22 , 26 , 27 ]. However, it is difficult to implement proper control measures at slaughterhouse level if the epidemiology of Campylobacter in swine is unclear [ 28 , 29 ]. Traditionally, C. jejuni has been linked to food infection from chicken meat consumption [ 30 ], while Campylobacter coli ( C. coli) has been linked to food infection by swine [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

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Multidrug-Resistant Campylobacer jejuni on Swine Processing at a Slaughterhouse in Eastern Spain

Marín

Lorenzo-Rebenaque

Moreno-Moliner

et al. 2021

Animals

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Campylobacteriosis is the most commonly reported gastrointestinal disease in humans in the EU, mainly from poultry meat consumption. C. jejuni is the main species involved in the human disease. However, little is known about the role of swine meat in its epidemiology. Thus, the aim of this study was to assess the epidemiology and antimicrobial resistance of C. jejuni on swine processing at the slaughterhouse. To this end, a total of 21 pig herds were intensively sampled at the slaughterhouse. Campylobacter isolation was based on official method ISO 10272-1:2018, speciation was determined by the hippurate hydrolysis test, and antibiotic susceptibility was performed according to standard disc diffusion assay. The results showed that all batches shed Campylobacter in faeces upon arrival at the slaughterhouse and remained positive at the end of the slaughtering process (42.8%). Moreover, 41.5% of Campylobacter strains isolated were C. jejuni and all of them were resistant to at least one antibiotic, and 96.3% were multidrug-resistant strains. In conclusion, the high level of multidrug-resistant C. jejuni swine batch contamination at the slaughterhouse makes it necessary to include the swine sector in national control programmes to reduce the bacterium and its resistance.

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“…Durante estos diez años, en el grupo de investigación también hemos ido de la mano con el sector porcino, ya que en este sector destaca la ausencia de un plan nacional de control de Salmonella. Por ello, hemos puesto la mirada en la epidemiología de la bacteria a nivel de producción primaria y matadero, destacando la necesidad de la implementación de programas de control y medidas de bioseguridad en granja que logren la reducción de Salmonella en los productos derivados del sector porcino (Marin et al, 2020). Además, nuestros estudios han llegado más allá de la cadena de producción porcina, observándose que el empleo de carne de cerdo para la alimentación de buitres estaba dando lugar a la transmisión de Salmonella y otras bacterias, resistentes a antibióticos, convirtiéndolos en diseminadores (Marin et al, 2018a;Sevilla et al, 2020).…”

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Contamination of pig carcass with Salmonella enterica serovar Typhimurium monophasic variant 1,4[5],12:i:- originates mainly in live animals

Cited by 17 publications

References 27 publications

Highly prevalent MDR, frequently carrying virulence genes and antimicrobial resistance genes in Salmonella enterica serovar 4,[5],12:i:- isolates from Guizhou Province, China

Highly prevalent MDR, frequently carrying virulence genes and antimicrobial resistance genes in Salmonella enterica serovar 4,[5],12:i:- isolates from Guizhou Province, China

Multidrug-Resistant Campylobacer jejuni on Swine Processing at a Slaughterhouse in Eastern Spain

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