Contamination of fresh‐frozen plasma with viable white cells and proliferable stem cells

Prestorage Leukocyte Depletion with In-Line Filtration of Whole Blood in Comparison with Blood Component Leukocyte Depletion

“…The experiments of Blajchman et al [15] showed that even fresh plasma could induce refractoriness to platelet transfusions in rabbits. Recently, we demonstrated that fresh frozen and thawed plasma con tains significant amounts of intact and even proliferable cells [16]. The filtration of whole blood did not only signif icantly reduce the WBC contamination, but also the platelet count.…”

Clinical Impact of Bioactive Substances in Blood Components: Implications for Leukocyte Filtration

“…In addition, preparation was shown to lead to platelet activation [72,103]. Fresh plasma prepared for fresh frozen plasma may also be contaminated by leukocytes and platelets [104], which by the freezing/thawing process may contaminate the plasma with bioactive substances before transfusion [105].…”

Fresh Frozen Plasma and Related Products

“…123,[128][129][130] Whereas pathogenic alloantibodies are typically of donor origin, in fewer than 10% of cases, the alloantibodies may be of recipient origin, directed against donor-specific leukocyte alloantigens. Several reports have documented the presence of leukocytes in FFP, [143][144][145][146] which has led to questions regarding their clinical relevance as well as the need for further processing (e.g., leukoreduction, irradiation) of FFP to reduce the risks of leukocyte-associated complications. 131,137,138 Alloantibodies to HLA class I and class II antigens have been detected in approximately 22% of blood components tested.…”