Contact Sensitivity in the Pig

McFarlin, Dale E.; Balfour, Brigid M.

doi:10.1007/978-1-4615-9017-0_78

Cited by 19 publications

(21 citation statements)

References 8 publications

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“…Grabbe et al (1994) showed that inbred miniature pigs possess skin APCs which function in the same way as human and murine LCs. In addition, other studies have shown that porcine skin DCs are important in delayed hypersensitivity reactions (Grabbe et al, 1994;McFarlin and Balfour, 1973) and are involved in allergic contact dermatitis (Vana and Meingassner, 2000). Our data show that porcine skin DCs transcribe CD207/langerin mRNA and express langerin protein, which is reactive with antibodies raised against human langerin and that the majority of DCs in porcine skin epithelium are LCs.…”

Section: Discussionsupporting

confidence: 63%

Langerhans cells in porcine skin

Nfon

Dawson

Toka

et al. 2008

Veterinary Immunology and Immunopathology

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Section: Discussionsupporting

confidence: 63%

Langerhans cells in porcine skin

Nfon

Dawson

Toka

et al. 2008

Veterinary Immunology and Immunopathology

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“…In addi tion, these works documented the ultrastructural characteristics of such cells, commented on their con tent of ingested material, noted and described the close contacts that they make with lymphocytes, and showed that some of them were capable of phagocytosing carbon particles in vivo. Similar cells have been described since in pigs [7], rabbits [8], and man [9], However, the characteristic ruffled surface mem brane of these cells can only be seen easily when liv ing cells are viewed with phase-contrast optics and rarely remains visible in specimens fixed and stained by conventional haematological methods [10], where the cells appear usually as unremarkable monocytes or macrophages. A further complication is that in those species whose epidermal Langerhans cells con tains Birbeck granules -e.g., rabbits [8] and man [9] a minority of the lymph-borne dendritic cells contain Birbeck granules too.…”

Section: Introductionmentioning

confidence: 93%

Phagocytosis, in vivo, of Immune Complexes by Dendritic Cells in the Lymph of Sheep

Jg¹,

Robertson²

1984

Int Arch Allergy Immunol

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Although the occurrence of dendritic, macrophage-like cells in the peripheral lymph of sheep, pigs, rabbits, and man is well documented, the exact nature of these cells has become controversial. Generally, such cells tend not to adhere firmly to glass or exhibit phagocytosis in in vitro systems. However, when immunized sheep were given a subcutaneous injection of specific antigen (horseradish peroxidase), most of the macrophage-like cells in the lymph from the injection site could be shown by transmission electron microscopy to have engulfed the immune complexes that had been formed in the subcuticulum.

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“…In the uppermost portion of the dermis, vasodilatation, perivascular edema, and cellular infiltration were present, overall scored at 17.0 (12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29). Subsequently, these changes increased and reached maximal intensity 24-48 hours after challenge (scores 31.5 and 33.0 [27][28][29][30][31][32][33][34][35][36], respectively). The epidermis showed pronounced acanthosis associated with many mitotic or vacuolated keratinocytes and moderately heavy spongiosis.…”

Section: Histopathologymentioning

confidence: 99%

“…25,37,51 For previously mentioned reasons domestic pigs or minipigs are increasingly used for preclinical studies in investigative dermatology, which in turn has resulted in increased availability of new investigative tools such as monoclonal antibodies to cell surface markers or cytokines. 17,24,34,40,46,49,55,59,61 In addition, the possible use of genetically altered pigs as organ donors for humans created tremendous interest in swine immunology as an investigative tool.…”

mentioning

confidence: 99%

Morphologic and Immunohistochemical Features of Experimentally Induced Allergic Contact Dermatitis in Göttingen Minipigs

Vana

Meingassner

2000

Vet Pathol

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Abstract. Many preclinical studies in investigative dermatology are performed preferably in pigs because pig skin is more similar to human skin than is rodent skin. A frequently used model is allergic contact dermatitis (ACD); however, this T-cell-mediated skin condition so far is not well characterized in pigs. The present study is aimed at the evaluation of morphologic and immunohistochemical features of experimentally induced acute ACD in Göttingen minipigs using 2,4-dinitrofluorobenzene (DNFB) as a hapten. Eight minipigs were sensitized with 10% DNFB and challenged 2 weeks later at different sites with 1% DNFB. In addition to clinical examinations, cutaneous blood flow was quantified by laser Doppler velocimetry (Periflux PF3). These examinations were performed before challenge and 8, 24, 48, and 72 hours after challenge. Skin biopsies were taken at the same time points, fixed, sectioned, and stained with Giemsa for histologic evaluation, or with mouse anti-swine monoclonal antibodies (CD1, CD2, CD4, CD5, CD8, CD25, CD45, MHCII) and with one mouse anti-human monoclonal antibody (CD62E) cross-reacting with swine for immunohistochemical evaluation. Positively stained cells were counted per square millimeter of epidermis and dermis by using a video image analyzing system (Videoplan Kontron). Erythema and cutaneous blood flow peaked at 24 hours. The major epidermal changes most pronounced at 48 hours were acanthosis, spongiosis, intracellular edema, exocytosis, and abscesses mainly containing neutrophils and mononuclear cells (MNC). Perivascular infiltrates of MNC as well as neutrophils and eosinophils were the most significant dermal changes, with peak levels at 24-48 hours. In biopsies taken before challenge, CD1ϩ dendritic cells were found in similar numbers and locations as MHCII ϩ cells in the epidermis. In the epidermis the maximum CD1 ϩ cell decrease occurred at 24 hours whereas in the dermis the maximum increase in CD1 ϩ stained cells was seen at 72 hours. The dermal infiltrate (CD2 ϩ , CD5 ϩ , CD25 ϩ , and CD45 ϩ ) was most dense at 48 hours. Between 8 and 48 hours more CD4 ϩ were present than CD8 ϩ cells, whereas at 72 hours CD4 ϩ and CD8 ϩ cells were similar in numbers. These findings closely resemble changes in human ACD. Therefore, DNFB-induced ACD in Göttingen minipigs is considered to be an appropriate animal model to study immunopathologic mechanisms and pharmacologic intervention.Key words: Allergic contact dermatitis; 2,4-dinitrofluorobenzene; Göttingen minipig; histopathology; immunohistochemistry; skin inflammation; swine.Preclinical research in biomedical science uses laboratory animals to profile new compounds for possible uses as drugs. Therefore, laboratory animals serve either as models for the assessment of pharmacodynamic effects or, in advanced stages of drug development, for the identification of untoward effects (toxicities) of drug candidates. Therefore, the results obtained from animal pharmacology or animal toxicology studies should be reliably predictive for the situation in hum...

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Contact Sensitivity in the Pig

Cited by 19 publications

References 8 publications

Langerhans cells in porcine skin

Langerhans cells in porcine skin

Phagocytosis, in vivo, of Immune Complexes by Dendritic Cells in the Lymph of Sheep

Morphologic and Immunohistochemical Features of Experimentally Induced Allergic Contact Dermatitis in Göttingen Minipigs

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