Phagocytosis, in vivo, of Immune Complexes by Dendritic Cells in the Lymph of Sheep

Jg, Hall; Robertson, Darrell A.

doi:10.1159/000233457

Cited by 19 publications

(18 citation statements)

References 13 publications

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“…A second possibility is that DC bind antigen via cytophilic immunoglobulin or via uptake of antigedantibody complexes. Recent independent studies in sheep [7,26], have confirmed earlier observations [28] that afferent lymph DC bear surface IgM and IgG molecules, and can take up immune complexes [29].…”

Section: Discussionsupporting

confidence: 65%

Uptake of antigen by afferent lymph dendritic cells mediated by antibody

1990

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Dendritic cells isolated from sheep afferent lymph were examined for their ability to bind soluble protein and peptide antigens labeled with fluorescein both in in vitro assays and following intradermal injection of antigen in vivo. Analysis of dendritic cells by flow cytometry revealed weak direct binding of proteins and peptide antigens. However, the degree of uptake was greatly enhanced in the presence of specific antibody in vitro or if antigen was injected intradermally into antigen-primed sheep. About 60% of dendritic cells possessed the ability to take up antigen in both the in vitro and in vivo experiments. The uptake of antigen occurred very rapidly, reaching maximum values in terms of cell numbers and fluorescence intensity in less than 5 min in vitro and 20-40 min following in vivo challenge. Both sheep IgG subclasses could mediate this effect, but F(ab')2 fragments were ineffective. Procedures adopted to remove complement components from the in vitro test mixtures did not result in any reduction in the binding of antigen by dendritic cells. Two-color flow cytometry analysis of the dendritic cell population further showed that 43% of cells taking up the antigen/antibody complexes were CD1+, suggesting a relationship between these cells and Langerhans' cells or other dendritic cells in skin. The results, thus, indicate that approximately two thirds of sheep afferent lymph dendritic cells bind antigen/antibody complexes via an Fc receptor, a mechanism which could be important in the accentuated accessory function of these cells known to occur following secondary antigen challenge.

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Section: Discussionsupporting

confidence: 65%

Uptake of antigen by afferent lymph dendritic cells mediated by antibody

1990

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“…Skin paint- ing with FITC induced a population of DC in the draining lymph node capable of sensitizing antigen-specific T cells and inducing sensitivity in naive recipients [8]. D C migration is not exclusively restricted to skin versus skinassociated lymph nodes, but actively functions between other tissues [22], such as intestine and MLN, as observed by Liu and MacPherson [23, 241. These investigators, using mesenteric lymphadenectomy in the rat, clearly demonstrated that intestinal DC migrated from the gut to distant lymphoid organs.…”

Section: Development Of Potent Immunostimulatory DC Upon Stimulation mentioning

confidence: 99%

Maturation of Peyer's patch dendritic cells in vitro upon stimulation via cytokines or CD40 triggering

Ruedl¹,

Hubele²

1997

Eur J Immunol

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In mouse Peyer's patches (PP), dendritic cells (DC) are localized in T cell areas as NLDC145+ CD11c+ cells, and in the dome and corona region of the follicle as NLDC145- CD11c+ cells, respectively, suggesting the presence of two different DC populations with distinct roles in antigen uptake, processing, and presentation. However, it is not clear how this relates to DC maturation. In this report, we demonstrate that freshly-isolated CD11c+ DC have the properties of immature DC since they endocytose soluble antigens, phagocytose particulate material such as latex beads, synthetize major histocompatibility complex (MHC) class II and invariant chain, but, at the same time, display low stimulatory activity for resting T cells, as shown in mixed-lymphocyte reaction and oxidative mitogenesis assays. When cultured for 24 h in the presence of the cytokines granulocyte-macrophage colony-stimulating factor and tumor necrosis factor or anti-CD40, the cells undergo dramatic phenotypic and functional changes characteristic of DC maturation. After 24 h stimulation in vitro, CD11c+ cells lose the ability to take up proteins such as ovalbumin, and in parallel with this decline, the biosynthesis of MHC class II and invariant chain is dramatically down-regulated or eliminated. On the other hand cells treated in vitro exhibit on the cell surface higher levels of MHC class II, of co-stimulatory molecules (CD80, CD86), of adhesion molecules (CD44, intercellular adhesion molecule-1), and acquire expression of the interdigitating DC surface marker NLDC145. Concomitantly, the ability to stimulate naive T cells drastically increased after in vitro treatment with both stimuli. Taken together, our results indicate that the majority of DC in the PP are immature in terms of their antigen-uptake capacity. These sentinel antigen presenting cells are strategically positioned at the dome region of PP, where antigens are transcytosed via the M cells from the gut lumen. A second population of mature interdigitating NLDC145+ CD11c+ DC stimulates naive unprimed T cells in interfollicular areas by up-regulation of surface ligands and accessory signals.

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“…Studies on peripheral lymph draining from several body regions of sheep have established that the extent of lymphocyte recirculation is lower in nonlymphoid tissues than in lymphoid, and that peri pheral lymph typically contains 5-20% of macro phages and so-called 'veiled' cells, most of which have the ability to engulf immune complexes in vivo [1,2].…”

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confidence: 99%

Cellular Composition of Peripheral Lymph and Skin of Sheep Defined by Monoclonal Antibodies

Hein

McClure²,

Miyasaka³

1987

Int Arch Allergy Immunol

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The surface phenotype of cells in peripheral lymph collected from afferent lymphatics leading to the popliteal lymph node of sheep was determined using a panel of monoclonal antibodies (mAbs). The majority of lymphocytes (83.5%) expressed the sheep pan-T cell antigen and only 13.3% bore surface immunoglobulin molecules. All peripheral T cell subsets occurring in sheep were detected; 50.2% of lymphocytes were positive for mAb SBU-T4 (T helper), 7.3% were positive for mAb ST-8 (T cytotoxic), and 8.4 and 43.0% expressed T subset markers recognized by mAbs 197 and T-80, respectively. Major histocompatibility complex (MHC) class I antigens were detected on 71.1% of lymphocytes and MHC class II antigens on 21.8%. The macrophage/veiled cells found in peripheral lymph did not express lymphocyte subset markers but were positive for MHC class I and II antigens, the sheep homologue of T6 antigen, leukocyte common antigen and mAb 175 (myeloid/erythroid). Macrophage-like cells occurring in the epidermis of skin taken from the lower hindleg gave positive staining reactions to the same mAbs which stained the macrophage/veiled cells in peripheral lymph. These results illustrate differences between the migration of lymphocyte subsets through nonlymphoid as compared to lymphoid tissues and point to a possible developmental or migratory relationship between the macrophage-like cells in skin and those in afferent popliteal lymph.

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Phagocytosis, in vivo, of Immune Complexes by Dendritic Cells in the Lymph of Sheep

Cited by 19 publications

References 13 publications

Uptake of antigen by afferent lymph dendritic cells mediated by antibody

Uptake of antigen by afferent lymph dendritic cells mediated by antibody

Maturation of Peyer's patch dendritic cells in vitro upon stimulation via cytokines or CD40 triggering

Cellular Composition of Peripheral Lymph and Skin of Sheep Defined by Monoclonal Antibodies

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