Contact Line Deposits on cDNA Microarrays:  A “Twin-Spot Effect”

Blossey, Ralf; Bosio, Andreas

doi:10.1021/la0114732

Cited by 112 publications

(108 citation statements)

References 10 publications

(15 reference statements)

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“…Using the SECM, the deposited droplet was scanned with an 8 mm diameter glutamate ultramicrobiosensor. As shown in Figure 5, higher glutaminase activity was revealed at the edge of the enzyme spot due to the "doughnut effect" [34] proving that the developed glutamate ultramicrobiosensors are Besides appropriate size and sensitivity, an adequate selectivity is of equal importance. In vivo measurements are very demanding in this respect (the extracellular space being of complex and changing composition), but cell cultures (characterized by a well-controlled environment and somewhat predictable reactions) also require good selectivity.…”

Section: à2mentioning

confidence: 94%

Ultramicrobiosensor for the Selective Detection of Glutamate

2007

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Carbon fiber microelectrodes, able to detect catecholamine release from single cells, have significantly contributed to our present understanding of the mechanism of secretory neurotransmission. In spite of their obvious advantages, there are only a few amperometric sensors (characterized by appropriate size, sensitivity, and selectivity) able to measure the release of other (not easily oxidizable) neurotransmitters at cellular level. The present work describes the fabrication and characterization of an ultramicrobiosensor for the selective detection of glutamate. The developed sensor has a size of 2.5 -15 mm in diameter, a sensitivity of 0.62 mA mM À1 cm À2 , and a detection limit of 5 mM. The excellent selectivity of the sensor (achieved using electrodeposition of Ru, Rh, and poly(m-phenylenediamine)) makes it a promising candidate for monitoring glutamate release at single cell level.

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Section: à2mentioning

confidence: 94%

Ultramicrobiosensor for the Selective Detection of Glutamate

2007

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“…These include the spray cooling process, 2,13 DNA stretching, 3-5, 7, 14 thin film deposition, [15][16][17][18][19] and the fabrication of patterned surfaces inspired by the coffee-stain phenomenon. [20][21][22] This fabrication of patterned surfaces has various applications such as DNA/RNA microarrays, 23,24 ordering and assembling of colloidal particles by evaporation, 6,8,25,26 fabrication of microlenses, 1,27,28 and electronic devices fabricated by the ink-jet printing method. 5,[29][30][31] Several kinds of fluid flow are autonomously generated inside an evaporating droplet.…”

Section: Introductionmentioning

confidence: 99%

Evaporation-induced saline Rayleigh convection inside a colloidal droplet

et al. 2013

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Inside evaporating two-component sessile droplets, a family of the Rayleigh convection exists, driven by salinity gradient formed by evaporation of solvent and solute. In this work, the characteristic of the flow inside an axisymmetric droplet is investigated. A stretched coordinate system is employed to account for the effect of boundary movement. A scaling analysis shows that the flow velocity is proportional to the (salinity) Rayleigh number (Ras) at the small-Rayleigh-number limit. A numerical analysis for a hemispherical droplet exhibits the flow velocity is proportional to the non-dimensional number \documentclass[12pt]{minimal}\begin{document}$Ra_s^{1/2}$\end{document}Ras1/2, at high Rayleigh numbers. A self-similar condition is established for the concentration field irrespective of the Rayleigh numbers after a moderate time, and the flow field is invariant with time at this stage. The scaling relation for the high Rayleigh numbers is verified experimentally by using aqueous NaCl droplets.

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“…Recent successes include preparing fibers and ribbons of SWNTs; [2] films of pure SWNTs, [3,4] polymers doped with SWNTs, [5,6] and growth in situ of SWNT arrays. [7] Evaporation of drops on substrates has been used for patterned deposition of solutes onto non-porous substrates, such as in DNA microarrays, [8] nanolithography, [9] protein crystallization, [10] and stretching DNA for hybridization studies. [11,12] Shimoda et al [13] prepared continuous selfassembled films of SWNT bundles on glass near a receding contact line by solvent evaporation.…”

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confidence: 99%

Self‐Assembly of Single‐Walled Carbon Nanotubes into a Sheet by Drop Drying

2005

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Single-walled carbon nanotubes (SWNTs) are currently the focus of extensive interdisciplinary studies because of their unique physical and chemical properties and potential electronic applications, for example, in making sensors and fieldemission devices.[1] Processing of SWNT-based materials into engineered macroscopic materials is still in its infancy; the most successful methods so far have been based on adapting techniques that had been developed in other areas of material science such as colloids and polymers. Recent successes include preparing fibers and ribbons of SWNTs; [2] films of pure SWNTs, [3,4] polymers doped with SWNTs, [5,6] and growth in situ of SWNT arrays. [7] Evaporation of drops on substrates has been used for patterned deposition of solutes onto non-porous substrates, such as in DNA microarrays, [8] nanolithography, [9] protein crystallization, [10] and stretching DNA for hybridization studies. [11,12] Shimoda et al. [13] prepared continuous selfassembled films of SWNT bundles on glass near a receding contact line by solvent evaporation. The moving contact line of a drying drop could be similarly used to form aligned patterns of SWNTs on substrates for making films or for nanofabrication. Drops of a solution on a substrate follow one of two drying mechanisms: either the drop maintains a constant contact angle by de-pinning the contact line (e.g., water on non-wetting substrates [14] ), or the contact line gets pinned and the drop maintains a fixed contact area (e.g., colloidal dispersions [15] ).Deegan and co-workers [15][16][17] have studied the drying of drops of colloidal dispersions and found that the particles deposit in a ring at the periphery of the drop due to capillary flow in which the pinned contact line causes the solvent to flow towards the edge. Recent investigations have also shown the formation of a skin or crust at the free surface of drops of polymers and colloidal suspensions. [18,19] Pauchard and Allain [19] found that the crust may collapse and evolve into different shapes as the surface area remains constant while the drop volume decreases due to solvent evaporation. "Crusting" on the surface of spin-cast films [20,21] is a well-known phenomenon.De Gennes [22] suggested a transport model for crust formation in spin-cast films. Because the glass-transition or gelation temperature of a pure polymer/colloid is higher than that in solution, [19] at any temperature below the glass transition there is a critical particle concentration at which the system transitions from fluid to glassy or gel-like. Evaporation of solvent from the free surface leads to a local increase in concentration of the polymer/suspension at the free surface, and a very thin glassy or gelled crust is formed at the free surface. Here, we investigated drying of a sessile drop of individually suspended SWNTs in an aqueous solution of F68 Pluronic. We found that, instead of assembling on the substrate, the SWNTs selfassemble into a crust at the free surface. This entangled meshlike crust was characterized b...

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Contact Line Deposits on cDNA Microarrays: A “Twin-Spot Effect”

Cited by 112 publications

References 10 publications

Ultramicrobiosensor for the Selective Detection of Glutamate

Ultramicrobiosensor for the Selective Detection of Glutamate

Evaporation-induced saline Rayleigh convection inside a colloidal droplet

Self‐Assembly of Single‐Walled Carbon Nanotubes into a Sheet by Drop Drying

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