Consumption of green tea protects rats from exercise-induced oxidative stress in kidney and liver

Determination of malondialdehyde (MDA) by high-performance liquid chromatography in serum and liver as a biomarker for oxidative stressApplication to a rat model for hypercholesterolemia and evaluation of the effect of diets rich in phenolic antioxidants from fruits

“…Data in the literature on MDA content in rat liver (0.5-0.7 nmol/mg of liver protein in control animals, analysed as TBARS) [21,24], were not very different from the values obtained here. Some other authors, however, report values as high as 200-300 nmol/g of rat liver [20,23], even up to 25 mol/g of liver in mice [26].…”

“…These authors found a significant reduction of TBARS when animals were given increasing amounts of green tea polyphenols in the diet (up to 2.5%) for 5 weeks, although final values were always higher than in controls. Green tea polyphenols also decreased MDA levels (as TBARS) in the liver of rats subjected to exerciseinduced oxidative stress as compared to animals that drank water instead of tea, although basal levels of resting animals were not achieved [24]. In rat aorta, a decrease of TBARS in hypercholesterolemic animals was also observed upon supplementation of the diet with 0.1% of wine proanthocyanidins, yet again values were higher than in control normocholesterolemic animals [22].…”

“…BHT). In spite of this, TBARS is still widely used to determine MDA levels in human [15][16][17][18][19] and animal experiments [20][21][22][23][24][25][26], measuring MDA in biological samples such as plasma, or organs like liver, lung or kidney.…”

Determination of malondialdehyde by liquid chromatography as the 2,4-dinitrophenylhydrazone derivative

“…However, TBA reacts not only with MDA but with many other compounds of biological origin, therefore, derivatisation of MDA with 2,4-dinitrophenylhydrazine (DNPH) and conversion into pyrazole and hydrazone derivatives has been found to allow a more specific estimation of this compound, especially if combined with its separation using high-performance liquid chromatography (HPLC) [9][10][11]. This approach and similars have been widely used to determine MDA levels in a variety of biological samples, such as rat and human plasma [5,[12][13][14][15][16][17], rat liver and kidney [17,18], and other samples [19].…”

Inhibitory effect of epigallocatechin fromCamellia sinensisleaves against pro-inflammatory mediator release in macrophages

“…The polyphenols in green tea play an important role in imparting scent, color, and taste. It also has antioxidant, anti-aging, cancer cell growth inhibition, sterilization, and many other biological activities (Freudenberg 1920;Cowan 1999;Alessio et al 2002;Chang et al 2002). The catechins present in green tea are epigallocatechin gallate (EGCG), epigallocatechin (EGC), epicatechin gallate, epicatechin (EC), and others.…”