Determination of malondialdehyde by liquid chromatography as the 2,4-dinitrophenylhydrazone derivative

Mateos, Raquel; Goya, Luis; Bravo, Laura

doi:10.1016/j.jchromb.2004.02.004

Cited by 86 publications

(57 citation statements)

References 46 publications

(48 reference statements)

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“…They reported a concentration range of 20 to 200 µM of dexrazoxane in ameliorating effects that was similar to our protective concentration of dexrazoxane (100 and 200 µM). DNA damage and degradation of protein and lipids, and is mainly accepted as one of the most important risk factors in the development of chronic diseases (41). Another result of free radical generation is lipid peroxidation, which is believed to be one of the causes of cardiovascular disease and cancer (42).…”

Section: Discussionmentioning

confidence: 99%

Modulatory Effects of Dexrazoxane Against Genotoxicity and Lipid Peroxidation Induced by Idarubicin in HepG2 Cells

2018

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Background: Idarubicin is an anthracycline antibiotic drug widely used in chemotherapy. Dexrazoxane is an iron chelator used clinically against anthracyclines-induced cardiotoxicity. The present study was designed to determine the possible genoprotection of dexrazoxane on idarubicin-induced DNA damage and oxidative stress. Methods: In this study, the induction of DNA damage by idarubicin was examined on HepG2 cells, using comet assay. Cells were exposed to different concentrations of idarubicin in order to find the minimum and suitable genotoxic concentration. To survey the genoprotective effects of dexrazoxane, cells were subjected to several safe concentrations of dexrazoxane (10, 50, 100, and 200

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Section: Discussionmentioning

confidence: 99%

Modulatory Effects of Dexrazoxane Against Genotoxicity and Lipid Peroxidation Induced by Idarubicin in HepG2 Cells

2018

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“…On breakdown of such hydroperoxides a great variety of aldehydes can be formed (as secondary products). MDA, a three carbon compound formed by scission of peroxidized polyunsaturated fatty acids, is one of the main secondary products of lipid peroxidation [16,22] . MDA is reactive toward amino groups of protein and nucleic acid and have cytotoxic and mutagenic effects.…”

Section: Discussionmentioning

confidence: 99%

“…MDA is reactive toward amino groups of protein and nucleic acid and have cytotoxic and mutagenic effects. Since MDA has been found elevated in various diseases thought to be related to free radical damage, it has been widely used as an index of lipid peroxidation in biological and medical sciences [20,22] . Deter mination of lipid peroxidation products in the body fluids can be used as a diagnostic tool for detecting increased levels of ROS.…”

Section: Discussionmentioning

confidence: 99%

Protective effect of melatonin against multistress condition induced lipid peroxidation via measurement of gastric mucosal lesion and plasma malondialdehyde levels in rats

Kiarostami¹,

Samini²,

Ghazi-Khansari³

2006

WJG

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AIM:To study the protective effect of a natural antioxidant, melatonin, against multistress condition induced lipid peroxidation via determination of gastric damage and plasma malondialdehyde (MDA) level by high performance liquid chromatography in rats. METHODS:We compared indomethacin-induced gastric damage and MDA plasma level in three groups of rats: unoperated, bile duct ligated and sham-operated and evaluated the role of the melatonin on gastric damage and plasma MDA level. Indomethacin and melatonin were injected intraperitoneally in doses of 50 mg/kg and 20 mg/kg, respectively. Animals were killed 4 h after indomethacin injection. RESULTS:Indomethacin induced more severe gastric damage and plasma MDA level in bile duct ligated animals was significantly higher (3.1 ± 0.04 µmol/L) than sham (2.8 ± 0.04 µmol/L) and unoperated animals (1.4 ± 0.08 µmol/L). Pretreatment with melatonin reduced indomethacin-induced gastric damage and plasma MDA level. CONCLUSION:Considering the results of this study, we suggest that in multistress conditions the intensity of gastric damage and the plasma MDA level are great and melatonin reduces the negative effect of lipid peroxidation and cell damage by oxidative stress in multistress conditions due to its antioxidizing activity.

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“…Cellular MDA was analyzed by HPLC as their DNPH derivatives. [29] An Agilent 1100 Series HPLC-DAD was used and MDA values were expressed as nmol of MDA/mg protein; total protein concentrations were estimated by the Bradford assay. [30] …”

Section: Determination Of Gpx and Gr Activitiesmentioning

confidence: 99%

Protective effects of papaya extracts on tert-butyl hydroperoxide mediated oxidative injury to human liver cells (An in-vitro study)

Tan

Ramos

Mateos

et al. 2012

Free Radicals and Antioxidants

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Determination of malondialdehyde by liquid chromatography as the 2,4-dinitrophenylhydrazone derivative

Cited by 86 publications

References 46 publications

Modulatory Effects of Dexrazoxane Against Genotoxicity and Lipid Peroxidation Induced by Idarubicin in HepG2 Cells

Modulatory Effects of Dexrazoxane Against Genotoxicity and Lipid Peroxidation Induced by Idarubicin in HepG2 Cells

Protective effect of melatonin against multistress condition induced lipid peroxidation via measurement of gastric mucosal lesion and plasma malondialdehyde levels in rats

Protective effects of papaya extracts on tert-butyl hydroperoxide mediated oxidative injury to human liver cells (An in-vitro study)

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