Consumption of a high-fat, high-calorie meal is associated with an increase in intracellular co-localization of PPAR-γ mRNA and protein in monocytes

Cancer‐related mRNA expression analysis using a novel flow cytometry‐based assay

“…To the best of our knowledge, this is the first report describing this technique in a cancer-related setting, and only a handful reports have addressed its application worldwide (20)(21)(22)(23)(24)(25). Subsequently, guidelines are lacking on how to interpret the mRNA amplification signal.…”

“…Only a handful of reports have documented its application and, to the best of our knowledge, cancer-related research has not yet been addressed (20)(21)(22)(23)(24)(25). Herein, the single-cell resolution of FCM is combined with a hybridization-based branched DNA (bDNA) technology, allowing intracellular RNA detection followed by exponential signal amplification.…”

“…Herein, the single-cell resolution of FCM is combined with a hybridization-based branched DNA (bDNA) technology, allowing intracellular RNA detection followed by exponential signal amplification. Only a handful of reports have documented its application and, to the best of our knowledge, cancer-related research has not yet been addressed (20)(21)(22)(23)(24)(25). Here, we evaluated the feasibility of PrimeFlow TM RNA assay in detecting key target mRNAs in NB and AML, using cell lines and patient samples, respectively.…”

Epigenetic regulation in monocyte/macrophage: A key player during atherosclerosis

“…Besides, unhealthy diet such as high carbohydrate and mediterranean diets can cause the down‐regulation of tissue factor in circulating monocytes . High‐calorie, high‐fat meal can alter the sensitivity of monocytes to modified LDL . Besides, other elements that affect the progression of atherosclerosis by targeting monocyte/macrophage are summarized in Table .…”

“…34 High-calorie, high-fat meal can alter the sensitivity of monocytes to modified LDL. 35 Besides, other elements that affect the progression of atherosclerosis by targeting monocyte/ macrophage are summarized in Table 1.…”