Consumo de alcohol en ratas adolescentes tratadas con reserpina y fluoxetina

Amphetamine, but not methylphenidate, increases ethanol intake in adolescent male, but not in female, rats

“…Ethanol intake in rats can be driven by DA deficits in the nucleus accumbens (Feltmann, Fredriksson, Wirf, Schilstrom, & Steensland, 2016), and ethanol craving in humans is associated with low levels of DA synthesis in the dorsal striatum (Heinz et al, 2005). It also worth noting that the rationale for focusing our measurements at the insula was that adolescent rats with low insular levels of DA exhibited depression-like behavior and increased ethanol intake (Ruiz et al, 2017 & Gysling, 2014), and earlier work indicated DA depletion and loss of receptors in the dorsal striatum (Krasnova et al, 2001;Wagner, Ricaurte, Johanson, Schuster, & Seiden, 1980) and in the olfactory bulb (Atianjoh, Ladenheim, Krasnova, & Cadet, 2008), after repeated AMPH treatment. It is interesting to remark that termination of a 6-day AMPH exposure to rats (5.0 or 10.0 mg kg −1 day −1 ) has been associated with depressive-like behavior and deficits in brain reward function, as indicated by increased intracranial self-stimulation (Cryan et al, 2003).…”

“…Moreover, a very limited monoamine analysis was conducted (i.e., only the insular levels were addressed). The rationale was that studies from our laboratory (Ruiz et al, 2017) and from others [e.g., (Diaz Heijtz & Castellanos, 2006) have suggested an association between neural activity or DA levels at this structure and ethanol intake or stimulant-induced behavioral activity.…”

“…We followed a procedure similar to that described in Ruiz et al (2017). Briefly, the animals were killed by decapitation.…”

“…Based on previous studies, however, we decided to focus on the insular cortex. We have recently observed that low levels of dopamine at the insula were associated, in adolescent Wistar rats, with heightened ethanol consumption (Ruiz, Calliari, & Pautassi, 2017). Also, Diaz Heijtz and Castellanos (2006) observed significantly greater insular c-fos expression after the administration of a D1 agonist, in spontaneously hypertensive rats (SHR, an animal model of ADHD) than in Wistar-Kyoto rats.…”

La Reserpina Aumenta la Expresión de BDNF y PCNA, y Disminuye la de Caspasa-3, en Células Intersticiales (Células de Leydig) de Ratas

“…La reserpina es un depletor de monoaminas que fue originalmente utilizada como antipsicótico e hipotensor arterial (Seeman & Lee, 1975;Shamon & Perez, 2009) y es también empleada para modelar los cuadros depresivos humanos en animales de laboratorio (Ruiz et al, 2017). Este modelo cuenta con críticas de constructo en referencia a la utilidad del mismo para estudiar la depresión en humanos (Leite Hunziker & Pérez-Acosta, 2001).…”