Background: In economic evaluation, the quality of life of people with a disability has traditionally been assessed by applying established generic preference-based measures. To provide robust measurement of the effectiveness of programs designed to improve the quality of life of people living with a disability, preference-based measures need to be sufficiently sensitive to detect incremental changes in the quality of life dimensions that are most important to people who have a disability.Methods: An online survey was developed and administered Australia wide to two different population samples. The first sample (n = 410) comprised adults (aged 18 years and above) with a disability (n = 208) and family carers of person/s with a disability (n = 202). The second sample included adults (aged 18 years and above) without disability (n = 443). Respondents were asked to rank the importance of 12 quality of life dimensions extracted from the content of established preference based quality of life measures (EQ-5D, AQoL and ASCOT) in terms of their relative importance to their overall quality of life. The results were combined, and the preference rankings between groups were compared.Results: People with a disability placed relatively higher importance on broader quality of life dimensions (e.g. Control, Independence, Self-care) relative to health status focused dimensions (e.g. Vision, Hearing, Physical mobility), whereas these two dimension categories were less clearly differentiable for the ‘without disability’ group. The biggest differences between the groups in what dimensions were ranked as the most important (i.e. 1st preference) were in: Vision (‘with disability’ = 10th, ‘without disability’ = 4th), Self-care (‘with disability’ = 3rd, ‘without disability’ = 7th) and Mental well-being (‘with disability’ = 6th, ‘without disability’ = 2nd) Conclusions: Quality of life preferences for people with a disability differ to those without a disability. As quality of life is a key outcome for economic evaluation and for assessing the impact of disability care policy and practice in Australia and internationally, it is important that new preference-based measures of quality of life are developed which are sufficiently sensitive to and incorporate the quality of life preferences of people with a disability.