Construction of vaccinia virus recombinants expressing several measles virus proteins and analysis of their efficacy in vaccination of mice

Wild, T. F.; Bernard, Arlette; Spehner, Danièle; Drillien, Robert

doi:10.1099/0022-1317-73-2-359

Cited by 70 publications

(36 citation statements)

References 46 publications

(42 reference statements)

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“…The only cross-reacting antibody induced by the W recombinants was directed against the F protein. No cross-neutralizing antibodies for any of the MV proteins were observed (Wild et al, 1992). Furthermore, the same protection was observed when the animals were challenged 9 days after vaccination suggesting that a CMI response may be involved.…”

Section: Discussionmentioning

confidence: 69%

“…The VV recombinants expressing the HA, F, NP and M proteins of MV, VV HA, VV-F, VV NP and VV-M respectively, are as previously described (Drillien et al, 1988;Wild et al, 1992Wild et al, , 1993. Viruses were grown in HeLa cells and titrated on Vero cells.…”

Section: Methodsmentioning

confidence: 99%

“…In a murine model (BALB/c), we showed that immunization with either the haemagglutinin (HA) or fusion (F) proteins of MV could protect animals against a fatal challenge (Drillien et al, 1988;Wild et al, 1992). In similar experiments challenging the mice with another morbillivirus, the closely related canine distemper virus (CDV), mice immunized with VV recombinants expressing the F protein, nucleoprotein (NP) or matrix (M) protein (VV-F, VV-NP and VV-M), were partially protected.…”

Section: Introductionmentioning

confidence: 99%

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Measles virus antigens induce both type-specific and canine distemper virus cross-reactive cytotoxic T lymphocytes in mice: localization of a common Ld-restricted nucleoprotein epitope

Beauverger

Buckland²,

Tf³

1993

Journal of General Virology

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We have studied the induction of the cytotoxic T lymphocyte (CTL) response to measles virus (MV) antigens expressed as vaccinia virus (VV) recombinants in a murine model. In C3H mice (H-2 k) only the nucleoprotein (NP) induced a CTL response and this was shown to be cross-reactive with the closely related canine distemper virus (CDV). The presentation of this antigen was shown to be Kk-restricted. In BALB/c mice (H-2d), both the haemagglutinin (HA) and the NP induced a strong CTL response, the former being serotype-specific, whereas the latter cross-reacted with CDV. Both responses were found to be La-restricted. Based on the prediction for L d T cell motifs, we tested a number of MV NP-derived nonapeptides for their capacity to sensitize P815 cells (H-2 o) for lysis by spleen cells from VV-NP-immunized mice. One of these peptides, comprising amino acids 281 to 289 (Tyr-ProAla-Leu-Gly-Leu-His-Glu-Phe) was as effective as cells expressing the complete NP protein. This motif is conserved in the CDV NP.

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Section: Discussionmentioning

confidence: 69%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Measles virus antigens induce both type-specific and canine distemper virus cross-reactive cytotoxic T lymphocytes in mice: localization of a common Ld-restricted nucleoprotein epitope

Beauverger

Buckland²,

Tf³

1993

Journal of General Virology

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“…This gene, designated KIL according to the nomenclature of Rosel et al (1986) for open reading frames (ORFs) on the HindIII restriction map of VV, was shown to encode an early protein ofM r 30K (Gillard et al, 1986). Unexpectedly, when the K1L gene was deleted from wt VV, the virus retained the ability to multiply in human cells but lost the capacity to multiply in RKI3 cells Wild et al, 1992). This suggested that another gene functionally equivalent to K1L for multiplication in human cells was present in the VV genome.…”

mentioning

confidence: 99%

“…To construct a recombinant virus lacking both C7L and K1L chick embryo fibroblasts were co-infected with VVIV043 and VV-NP. K1L is deleted in the latter, giving rise to plaques on permissive cells in which cell aggregation is considerably less extensive than in wt VV plaques (Wild et al, 1992). Recombinant virus that retained both the modifications introduced into the parental viruses could be recognized, therefore, by visual screening for the plaque morphology typical of a K1L-defective virus and for the blue plaque phenotype.…”

mentioning

confidence: 99%

Detection of a protein encoded by the vaccinia virus C7L open reading frame and study of its effect on virus multiplication in different cell lines

Oguiura

Spehner

Drillien

1993

Journal of General Virology

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Vaccinia virus encodes several proteins, the activity of which is essential for multiplication in different cell types. Both the C7L and K1L open reading frames (ORFs) have been characterized as viral determinants for multiplication in human cells. To confirm and extend these findings we inserted the C7L ORF into the genome of a mutant virus unable to multiply in human cells and showed that this virus recovered its ability to replicate. Deletion of C7L from a wild-type viral genome did not adversely affect virus multiplication in human cells but it did reduce replication in hamster Dede ceils. When both C7L and K1L were deleted from the vaccinia virus genome only poor or no viral yields were obtained from various human cell lines. Recombinant viruses were also constructed to facilitate the study of C7L protein synthesis during infection. One virus in which the lacZ ORF was fused downstream and in-frame with the C7L ORF enabled us to characterize the C7L protein as an early gene product. Another recombinant virus was constructed so that the carboxy terminus of the C7L ORF product contained an additional 28 amino acids from the carboxy terminus of K1L. Tagging of C7L in this way allowed us to detect the fusion protein by immunoprecipitation with antibodies against the K1L protein. Furthermore, the hybrid protein retained its biological properties. The recombinant viruses constructed in this work should be useful for studies of the molecular basis of the activity of viral host range proteins.

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Enhanced MHC class II-restricted presentation of measles virus (MV) hemagglutinin in transgenic mice expressing human MV receptor CD46

et al. 1998

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This study analyzes the role of the measles virus (MV) receptor, i.e. the human CD46 molecule, in the MHC class II-restricted presentation of MV hemagglutinin (H). We generated transgenic mice ubiquitously expressing CD46, with a similar level of transgene expression on the surface of antigen-presenting cells (APC), i.e. B cells, dendritic cells (DC) and macrophages. APC isolated from transgenic mice and nontransgenic controls were tested for their ability to present MV H to H-specific CD4 + I-E d -restricted T cell hybridomas. All three populations of APC were capable of presenting MV to T cell hybridomas, DC being the most efficient. Expression of CD46 on B lymphocytes increased MHC class II-dependent presentation of MV H up to 100-fold, while CD46-transgenic DC stimulated H-specific T cell hybridomas up to 10-fold better than nontransgenic DC. Interestingly, expression of CD46 did not change the presentation efficiency of transgenic macrophages, indicating that CD46-dependent enhancement of antigen presentation depends on the nature of the APC. Furthermore, a single injection of UV-inactivated MV particles into CD46-transgenic mice, but not nontransgenic controls, induced generation of MV-specific T lymphocytes and production of anti-H antibodies, suggesting a role for CD46 in the efficient capture of MV in vivo. These results show for the first time that one ubiquitously expressed cell surface receptor, like CD46, could function in receptor-mediated antigen presentation both in vitro and in vivo and its performance depends on the type of APC which expresses it.

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Construction of vaccinia virus recombinants expressing several measles virus proteins and analysis of their efficacy in vaccination of mice

Cited by 70 publications

References 46 publications

Measles virus antigens induce both type-specific and canine distemper virus cross-reactive cytotoxic T lymphocytes in mice: localization of a common Ld-restricted nucleoprotein epitope

Measles virus antigens induce both type-specific and canine distemper virus cross-reactive cytotoxic T lymphocytes in mice: localization of a common Ld-restricted nucleoprotein epitope

Detection of a protein encoded by the vaccinia virus C7L open reading frame and study of its effect on virus multiplication in different cell lines

Enhanced MHC class II-restricted presentation of measles virus (MV) hemagglutinin in transgenic mice expressing human MV receptor CD46

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