Construction of <scp>L</scp>‐Lysine Sensor by Layer‐by‐Layer Adsorption of <scp>L</scp>‐Lysine 6‐Dehydrogenase and Ferrocene‐Labeled High Molecular Weight Coenzyme Derivative on Gold Electrode

Zheng, Hongxing; Zhou, Jingli; Okezaki, Yosuke; Suye, Shin‐ichiro

doi:10.1002/elan.200804402

Cited by 11 publications

(8 citation statements)

References 33 publications

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“…Zheng and Suye’s group developed enzyme and DNA biosensors using Fc polymers [ 52 , 53 , 54 , 55 ]. They incorporated L-proline dehydrogenase and Fc-PAH into the LbL film through electrostatic affinity to construct L-proline sensors [ 54 ].…”

Section: Fc-containing Thin Filmsmentioning

confidence: 99%

“…For preparing L-lysine sensors, Zheng and Suye’s group synthesized a poly(ethylenimine) (PEI) derivative bearing coenzyme (nicotinamide adenine dinucleotide, NAD) and Fc moieties. They alternately deposited the Fc-NAD-bearing PEI and L-lysine dehydrogenase on an Au electrode to prepare reagentless L-lysine sensors, in which all the components (enzyme, coenzyme and electron-transfer mediator) were confined in the LbL film ( Figure 3 ) [ 55 ]. The L-lysine sensor exhibits an electrochemical response to L-lysine in the concentration range of 1–120 mM.…”

Section: Fc-containing Thin Filmsmentioning

confidence: 99%

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Recent Progress in Ferrocene-Modified Thin Films and Nanoparticles for Biosensors

Takahashi

Anzai

2013

Materials

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This article reviews recent progress in the development of ferrocene (Fc)-modified thin films and nanoparticles in relation to their biosensor applications. Redox-active materials in enzyme biosensors commonly use Fc derivatives, which mediate electron transfer between the electrode and enzyme active site. Either voltammetric or amperometric signals originating from redox reactions of Fc are detected or modulated by the binding of analytes on the electrode. Fc-modified thin films have been prepared by a variety of protocols, including in situ polymerization, layer-by-layer (LbL) deposition, host-guest complexation and molecular recognitions. In situ polymerization provides a facile way to form Fc thin films, because the Fc polymers are directly deposited onto the electrode surface. LbL deposition, which can modulate the film thickness and Fc content, is suitable for preparing well-organized thin films. Other techniques, such as host-guest complexation and protein-based molecular recognition, are useful for preparing Fc thin films. Fc-modified Au nanoparticles have been widely used as redox-active materials to fabricate electrochemical biosensors. Fc derivatives are often attached to Au nanoparticles through a thiol-Au linkage. Nanoparticles consisting of inorganic porous materials, such as zeolites and iron oxide, and nanoparticle-based composite materials have also been used to prepare Fc-modified nanoparticles. To construct biosensors, Fc-modified nanoparticles are immobilized on the electrode surface together with enzymes.

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Section: Fc-containing Thin Filmsmentioning

confidence: 99%

Section: Fc-containing Thin Filmsmentioning

confidence: 99%

Recent Progress in Ferrocene-Modified Thin Films and Nanoparticles for Biosensors

Takahashi

Anzai

2013

Materials

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“…[13][14][15][16][17][18][19] These interactions result in the formation of various types of charged polymer-protein complexes which have been applied for protein delivery, [20][21][22] protein separations, [23][24][25] and biosensors. [26][27][28] Such a complexation is also relevant to enzyme functions. For example, negatively charged amphiphilic polymers poly(isobutylene-alt-maleic acid) and poly(1-tetradecene-alt-maleic acid) bound positively charged lysozyme, which results in a loss of tertiary structure and enzymatic activity; after that the complex dissociates by the addition of NaCl or guanidine hydrochloride.…”

Section: Introductionmentioning

confidence: 99%

Enzyme switch by complementary polymer pair system (CPPS)

et al. 2010

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Manipulation of enzyme activity at will presents various research and industrial applications. Herein we describe development of a technology for inactivation and reactivation of enzyme activities using a polyanionic poly(acrylic acid) (PAAc) and a polycationic poly(allylamine) (PAA). Enzyme activities of ribonuclease A (RNase A), lysozyme, cellulase, and α-amylase were lost through addition of PAAc or PAA because of their binding to the enzymes. The activity of these enzymes except for α-amylase was then fully restored from the complex by the addition of oppositely charged polymers. Such manipulation of enzyme activity using a complementary polymer pair system (CPPS) enables the expansion of biomedical and biotechnological applications of the enzymes, including realization of protein delivery and intelligent bioreactors. 65

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“…On the other hand, high molecular weight coenzyme derivatives were also prepared [22] and used as an practical approach for the immobilization of coenzyme [23][24][25][26] . In our previous work, ferrocenelabeled high molecular coenzyme derivative were prepared and used in the fabrication of reagentless biosensors [27,28] . In this work, 3,4-dihydroxybenzaldehyde (3,4-DHB) was selected as the electroactive units, and a new functional polymer (PEI-DHB-NAD) was prepared by attaching both 3,4-DHB and native NAD + to the backbone of a water soluble polyelectrolyte, poly(ethylenimine).…”

Section: Introductionmentioning

confidence: 99%

Bioelectrochemical activity of an electroactive macromolecular weight coenzyme derivative

et al. 2009

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As coenzyme utilized by more than hundreds of dehydrogenases, the efficient immobilization and regeneration of nicotinamide adenine dinucleotide (NAD + ) are of great importance and have practical applications in industrial, analytical and biomedical field. In this paper, an electroactive macromolecular weight coenzyme derivative (PEI-DHB-NAD) was prepared by attaching both NAD + and 3,4-dihydroxybenzaldehyde (3,4-DHB) to a water-soluble polyelectrolyte, poly(ethylenimine) (PEI). The functional polymer exhibited both electrochemical properties of catechol unites and coenzymatic activity of NAD moieties. The macromolecular NAD analogue showed a substantial degree of efficiency relative to free NAD + with alcohol dehydrogenase (ADH) and glucose-6-phophate dehydrogenase (G6PDH), and a litter higher Michaelis-Menton constant (Km) was obtained for the coenzyme derivative than free NAD + . The bioelectrochemical properties of PEI-DHB-NAD were investigated by using G6PDH as the model enzyme, and both of them were retained on electrode surface by ultrafiltration membrane. The modified electrode showed typical response to substrate without the addition of free coenzyme, which indicated that PEI-DHB-NAD can carry out the electron transfer between electrode and NAD-dependent dehydrogenase. The utilization of polymer-based PEI-DHB-NAD is convenient for the immobilization of both electron mediator and coenzyme, and offers a practical approach for the construction of reagentless biosensors.

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Construction of L‐Lysine Sensor by Layer‐by‐Layer Adsorption of L‐Lysine 6‐Dehydrogenase and Ferrocene‐Labeled High Molecular Weight Coenzyme Derivative on Gold Electrode

Cited by 11 publications

References 33 publications

Recent Progress in Ferrocene-Modified Thin Films and Nanoparticles for Biosensors

Recent Progress in Ferrocene-Modified Thin Films and Nanoparticles for Biosensors

Enzyme switch by complementary polymer pair system (CPPS)

Bioelectrochemical activity of an electroactive macromolecular weight coenzyme derivative

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