Construction of scFv that bind both fibronectin-binding protein A and clumping factor A of Stapylococcus aureus

Wang, Man; Zhang, Yan; Li, Benqiang; Zhu, Jingde

doi:10.1016/j.rvsc.2015.02.012

Cited by 12 publications

(9 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Neutralizing monoclonal antibody against flu virus A was used in pig treatment [78]. Antibodies against fibronectin A binding protein and against the clumping factor A were used in cattle treatment [79], which prevented the development of mastitis in cattle. Antibodies against infectious bursal disease virus (IBDV) were used in poultry treatment [80].…”

Section: Monoclonal Antibodiesmentioning

confidence: 99%

Selected Biological Medicinal Products and Their Veterinary Use

Zygmuntowicz

Burmańczuk

Markiewicz

2020

Animals

View full text Add to dashboard Cite

Definitions of biological medicinal products (BMPs) vary depending on the source. BMPs are manufactured using complex biological/biotechnological processes involving living cell lines, tissues and organisms such as microorganisms, plants, humans and even animals. Advances in modern biotechnological methods and genetic engineering have made it possible to search for new drugs with a targeted effect and simultaneous reduction of adverse effects, which has resulted in BMPs dynamically increasing their share in the pharmaceutical market. Currently, these drugs are widely used in the treatment of many human diseases, but an increasing number of drugs of this group are also being used in the treatment of animals, mainly in dermatology, rheumatology and oncology. This article presents the current state of knowledge in the field of biological medicinal products used in animal therapy.

show abstract

Section: Monoclonal Antibodiesmentioning

confidence: 99%

Selected Biological Medicinal Products and Their Veterinary Use

Zygmuntowicz

Burmańczuk

Markiewicz

2020

Animals

View full text Add to dashboard Cite

show abstract

“…NM_001243319, respectively). The VH and VL sequences were amplified using the cDNA as template and combined with a DNA sequence encoding a (Gly 4 Ser) 3 peptide linker to form scFv fragments in an overlap PCR that was described by Wang et al [30]. The primer sequences are listed in Table 1.…”

Section: Phage Library Constructionmentioning

confidence: 99%

Construction and characterization of porcine single-chain fragment variable antibodies that neutralize transmissible gastroenteritis virus in vitro

et al. 2019

Self Cite

View full text Add to dashboard Cite

Transmissible gastroenteritis virus (TGEV) infection causes severe diarrhea in piglets and imposes a significant economic burden on pig farms. Single-chain fragment variable (scFv) antibodies effectively inhibit virus infection and could be a potential therapeutic reagent for preventing disease. In this study, a recombinant scFv antibody phage display library was constructed from peripheral blood lymphocytes of piglets infected with TGEV. The library was screened with four rounds of biopanning using purified TGEV antigen, and scFv antibodies that bound to TGEV were obtained. The scFv gene was subcloned into the pET-28a(+), and the constituted plasmid was introduced into Escherichia coli BL21 (DE3) for protein expression. All three scFv clones identified had neutralizing activity against TGEV. An immunofluorescence assay and western blot analysis demonstrated that two scFv antibodies reacted with the spike protein of TGEV. These results indicate that scFv antibodies provide protection against viral infection in vitro and may be a therapeutic candidate for both prevention and treatment of TGEV infection in swine.

show abstract

“…Tissue adhesion involves multiple proteins, including fibronectin-binding factor A and clumping factor A. These two proteins were targeted using a scFv to obstruct the adhesion mechanisms; therefore, they have potential to be used to prevent and treat bovine mastitis ( 55 ). Similarly, a sdAb that could recognize and neutralize β-hemolysin from S. aureus was generated and evaluated in vitro to confirm its neutralizing activity ( 56 ).…”

Section: Recombinant Antibodies For Therapeutic Purposesmentioning

confidence: 99%

Recombinant Antibodies in Veterinary Medicine: An Update

2018

View full text Add to dashboard Cite

The production of recombinant antibodies has had a tremendous impact on several research fields, most prominently in biotechnology, immunology and medicine, enabling enormous advances in each. Thus far, a broad diversity of recombinant antibody (rAb) forms have been designed and expressed using different expression systems. Even though the majority of rAbs approved for clinical use are targeted to humans, advances in veterinary medicine seem promising. The aim of this mini-review is to present an update regarding the rAbs in veterinary medicine reported to date, as well as their potential use in diagnostics, prophylaxis and therapeutics. Full- and single-chain fragment variables are the most common forms of rAbs developed for the detection, prevention and control of parasitic, bacterial and viral diseases, as well as pain and cancer treatment. Nonetheless, advances in research seem to be skewed toward economically important animals, such as pigs, cows, poultry and dogs. Although significant results have been obtained from the rAbs reported here, most have not been developed enough to be approved. Further research and clinical trials should be encouraged to enable important findings to fulfill their intended potential to improve animal well-being.

show abstract

Construction of scFv that bind both fibronectin-binding protein A and clumping factor A of Stapylococcus aureus

Cited by 12 publications

References 30 publications

Selected Biological Medicinal Products and Their Veterinary Use

Selected Biological Medicinal Products and Their Veterinary Use

Construction and characterization of porcine single-chain fragment variable antibodies that neutralize transmissible gastroenteritis virus in vitro

Recombinant Antibodies in Veterinary Medicine: An Update

Contact Info

Product

Resources

About