Construction of probe of the plant growth-promoting bacteria Bacillus subtilis useful for fluorescence in situ hybridization

Posada, Luisa F.; Álvarez, Javier Correa; Hu, Chia-Hui; de‐Bashan, Luz E.; Bashan, Yoav

doi:10.1016/j.mimet.2016.05.029

Cited by 16 publications

(6 citation statements)

References 29 publications

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“…In case of yeasts, partial 26S rRNA genes were amplified using the primers NL1 and NL4 ( Cocolin, 2000 ); for LAB species identification, the PCR was performed with primer pairs WLAB1 and WLAB2 targeted the V4 and V5 16S rDNA regions as López et al (2003) described. Finally, the AAB and the EB species identification was performed by amplification of the V1 to V6 region of 16S rDNA gene with 8F and 907R primers ( Posada et al, 2016 ). Macrogen Inc. (Seoul, South Korea) sequenced the PCR amplicons.…”

Section: Methodsmentioning

confidence: 99%

Impact of Chemical and Biological Fungicides Applied to Grapevine on Grape Biofilm, Must, and Wine Microbial Diversity

et al. 2018

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This study was aimed to measure the impact of the application of a bio-fungicide against Botrytis cinerea on the microbiota involved in the alcoholic fermentation (AF) of Tempranillo Rioja wines. For this purpose, a bio-fungicide composed of the biological control bacterium Bacillus subtilis QST713 was applied to the vineyard. The microbial diversity was analyzed from grape biofilm to wine. Impact on microbial diversity was measured employing indexes assessed with the software PAST 3.10 P.D. Results were compared to non-treated samples and to samples treated with a chemical fungicide mainly composed by fenhexamid. Overall, the impact of the biological-fungicide (bio-fungicide) on the microbial diversity assessed for grape biofilm and for musts was not remarkable. Neither of the tested fungicides enhanced the growth of any species or acted against the development of any microbial groups. The bio-fungicide had no significant impact on the wine microbiota whereas the chemical fungicide caused a reduction of microbial community richness and diversity. Although environmental threats might generate a detriment of the microbial species richness, in this study the tested bio-fungicide did not modify the structure of the microbial community. Indeed, some of the Bacillus applied at the grape surface, were detected at the end of the AF showing its resilience to the harsh environment of the winemaking; in contrast, its impact on wine quality during aging is yet unknown.

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Section: Methodsmentioning

confidence: 99%

Impact of Chemical and Biological Fungicides Applied to Grapevine on Grape Biofilm, Must, and Wine Microbial Diversity

et al. 2018

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“…FISH as an alternative technique allows the identification and visualization of individual microbe cells in natural environments by utilizing a fluorescentlabelled probe that hybridizes to specific target sequences within the intact cells (Levsky & Singer, 2003). In a study conducted by Posada et al (2016), a specific probe was designed for plant growthpromoting bacterium B. subtilis EA-CB0575, where it successfully hybridized with several strains of B. subtilis and failed to hybridize with other closely related species. To avoid indigenous bacteria autofluorescence and root structure interference, a catalysed reporter deposition-FISH (CARD-FISH) methodology targeting this strain was further developed (Posada et al, 2018).…”

Section: Emphasizing Quantification: the Crucial Role Beyond Identifi...mentioning

confidence: 99%

How to identify and quantify the members of the Bacillus genus?

Xu,

Kovács

2024

Environmental Microbiology

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Members of the Bacillus genus are widely distributed throughout natural environments and have been studied for decades among others for their physiology, genetics, ecological functions, and applications. However, despite its prevalence in nature, the characterization and classification of Bacillus remain challenging due to its complex and ever‐evolving taxonomic framework. This review addresses the current state of the Bacillus taxonomic landscape and summarizes the critical points in the development of Bacillus phylogeny. With a clear view of Bacillus phylogeny as a foundation, we subsequently review the methodologies applied in identifying and quantifying Bacillus, while also discussing their respective advantages and disadvantages.

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“…A FISH-probe using Bacillus subtilis 16 s rRNA has been also reported with the aim of distinguishing targeted nucleotides between 465 and 483 genes [174]. However, the results showed that FISH method was not able to identify strains, i.e., B. altitudinis, B. cereus, B. gibsonii, B. pimulus and B. megaterium.…”

Section: Fluorescence In Situ Hybridization (Fish)mentioning

confidence: 99%

Detection of contaminants in water supply: A review on state-of-the-art monitoring technologies and their applications

Zulkifli

Rahim

Lau

2018

Sensors and Actuators B: Chemical

187

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a b s t r a c tWater monitoring technologies are widely used for contaminants detection in wide variety of water ecology applications such as water treatment plant and water distribution system. A tremendous amount of research has been conducted over the past decades to develop robust and efficient techniques of contaminants detection with minimum operating cost and energy. Recent developments in spectroscopic techniques and biosensor approach have improved the detection sensitivities, quantitatively and qualitatively. The availability of in-situ measurements and multiple detection analyses has expanded the water monitoring applications in various advanced techniques including successful establishment in hand-held sensing devices which improves portability in real-time basis for the detection of contaminant, such as microorganisms, pesticides, heavy metal ions, inorganic and organic components. This paper intends to review the developments in water quality monitoring technologies for the detection of biological and chemical contaminants in accordance with instrumental limitations. Particularly, this review focuses on the most recently developed techniques for water contaminant detection applications. Several recommendations and prospective views on the developments in water quality assessments will also be included.

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Construction of probe of the plant growth-promoting bacteria Bacillus subtilis useful for fluorescence in situ hybridization

Cited by 16 publications

References 29 publications

Impact of Chemical and Biological Fungicides Applied to Grapevine on Grape Biofilm, Must, and Wine Microbial Diversity

Impact of Chemical and Biological Fungicides Applied to Grapevine on Grape Biofilm, Must, and Wine Microbial Diversity

How to identify and quantify the members of the Bacillus genus?

Detection of contaminants in water supply: A review on state-of-the-art monitoring technologies and their applications

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