“…Among these, the Gateway Ò cloning technology has attracted molecular biologists from different disciplines due to its amenability and robustness (Schoberle et al, 2013). To date, a few methods or constructs have been developed using this technology for the functional analyses of genes in plant pathogenic fungi (Abe et al, 2006;Nakagawa et al, 2007;Paz et al, 2011;Shafran et al, 2008;Zhu et al, 2009). For instance, the One Step Construction of Agrobacterium-Recombination-ready-plasmids (OSCAR) has been developed to create deletion constructs for Agrobacterium tumefaciens mediated transformation (Paz et al, 2011).…”