Construction of PAH-degrading mixed microbial consortia by induced selection in soil

Zafra, German; Absalón, Ángel E.; Anducho-Reyes, Miguel Angel; Fernandez, F; Cortés-Espinosa, Diana V.

doi:10.1016/j.chemosphere.2016.12.038

Cited by 63 publications

(22 citation statements)

References 29 publications

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“…Recently different kinds of environmental pollutants were reported to be effectively degraded by microbial consortia. 23 , 24 , 25 , 26 , 27 The synergetic interaction of microorganisms in this study points out an alternative approach to perform rubber degradation. It was clearly shown that using mixed culture and the consortium resulted in better degradation.…”

Section: Discussionmentioning

confidence: 75%

Rubber gloves biodegradation by a consortium, mixed culture and pure culture isolated from soil samples

Nawong

Umsakul

Sermwittayawong

2018

Brazilian Journal of Microbiology

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An increasing production of natural rubber (NR) products has led to major challenges in waste management. In this study, the degradation of rubber latex gloves in a mineral salt medium (MSM) using a bacterial consortium, a mixed culture of the selected bacteria and a pure culture were studied. The highest 18% weight loss of the rubber gloves were detected after incubated with the mixed culture. The increased viable cell counts over incubation time indicated that cells used rubber gloves as sole carbon source leading to the degradation of the polymer. The growth behavior of NR-degrading bacteria on the latex gloves surface was investigated using the scanning electron microscope (SEM). The occurrence of the aldehyde groups in the degradation products was observed by Fourier Transform Infrared Spectroscopy analysis. Rhodococcus pyridinivorans strain F5 gave the highest weight loss of rubber gloves among the isolated strain and posses latex clearing protein encoded by lcp gene. The mixed culture of the selected strains showed the potential in degrading rubber within 30 days and is considered to be used efficiently for rubber product degradation. This is the first report to demonstrate a strong ability to degrade rubber by Rhodococcus pyridinivorans.

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Section: Discussionmentioning

confidence: 75%

Rubber gloves biodegradation by a consortium, mixed culture and pure culture isolated from soil samples

Nawong

Umsakul

Sermwittayawong

2018

Brazilian Journal of Microbiology

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“…and Penicillium sp. Fungal species isolated from soil contaminated with crude oil had the ability to degrade polycyclic aromatic hydrocarbon (Zafra et al, 2017). Similarly, fungal species were isolated from oil contaminated soil sources (Lotfinasabasl et al, 2012;Elango et al, 2014;Vanishree et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

Degradation of hydrocarbon by isolated fungal species with laccase activity

Gnanasekaran¹,

Iyyappan²,

Deepeka³

et al. 2019

JEB

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Hydrocarbon of crude oil and other oil products such as gasoline comprise aromatic, aliphatic and alicylic components pollute water bodies are hazardous. Microbial degradation of these oil spills are an efficient way. Degrading oil contamination by microorganism is the theme of this paper. To study degrading efficiency of crude oil hydrocarbon by fungi. Fungal species were isolated from crude oil soaked soil samples and oil contaminated soil samples from commercial oil industry. Isolated fungal species were cultured on nutrient media such as potato dextrose agar (PDA) and czapekdox agar at 37 ºC for 7 day. Further, fungal species were identified by 18 S rRNA sequencing. The potential of fungi to degrade hydrocarbon was investigated through laccase and peroxidase. To examine the ability to degrade oil, the fungal species were screened on Czapekdox agar and broth medium containing petroleum derivatives. Laccase activity was performed by ABTS (2, 2̍-azinobis 3ethyl-benzothiazoline-6-sulphonate) and bromophenol. They were used as substrate for screening laccase activity. Among the isolated eight fungal species, isolate FGS-4 showed better biodegradation ability and subsequent 18 S rRNA revealed that isolate FGS-4 belongs to Aspergillus tubigensis. Production of laccase enzyme was confirmed. The results of gas chromatography analysis showed that hydrocarbon was biodegraded by the isolated Aspergillus tubigensis. Biodegradation of hydrocarbon by Aspergillus species would create an impact on various waste treatment methods. Eco-friendly degradation of hydrocarbons could be achieved by Aspergillus species.

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“…Polycyclic aromatic hydrocarbons (PAH) constitute ubiquitous and serious worldwide pollutants since they are extremely harmful to human health (Vandermeersch et al 2015) and may drastically affect the biodiversity of natural ecosystems (Zhang et al 2015). Although several natural and anthropic sources contribute to the release of PAH into the environment, petrochemical activities account for the majority of those compounds contaminating soils and water bodies (Zafra et al 2017). Microbial degradation is the main process for the ecologic restoration of PAH-contaminated sites (Peng et al 2008); prokaryotes temporarily change their pattern of gene expression in response to environmental signals, such as sudden spikes in PAH (Fernandez-Luqueno et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Insights into the genome and proteome of Sphingomonas paucimobilis strain 20006FA involved in the regulation of polycyclic aromatic hydrocarbon degradation

Macchi

Martinez

Tauil

et al. 2017

World J Microbiol Biotechnol

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In order to study the mechanisms regulating the phenanthrene degradation pathway and the intermediate-metabolite accumulation in strain S. paucimobilis 20006FA, we sequenced the genome and compared the genome-based predictions to experimental proteomic analyses. Physiological studies indicated that the degradation involved the salicylate and protocatechuate pathways, reaching 56.3% after 15 days. Furthermore, the strain degraded other polycyclic aromatic hydrocarbons (PAH) such as anthracene (13.1%), dibenzothiophene (76.3%), and fluoranthene. The intermediate metabolite 1-hydroxy-2-naphthoic acid (HNA) accumulated during phenanthrene catabolism and inhibited both bacterial growth and phenanthrene degradation, but exogenous-HNA addition did not affect further degradation. Genomic analysis predicted 126 putative genes encoding enzymes for all the steps of phenanthrene degradation, which loci could also participate in the metabolism of other PAH. Proteomic analysis identified enzymes involved in 19 of the 23 steps needed for the transformation of phenanthrene to trichloroacetic-acid intermediates that were upregulated in phenanthrene cultures relative to the levels in glucose cultures. Moreover, the protein-induction pattern was temporal, varying between 24 and 96 h during phenanthrene degradation, with most catabolic proteins being overexpressed at 96 h-e. g., the biphenyl dioxygenase and a multispecies (2Fe-2S)-binding protein. These results provided the first clues about regulation of expression of phenanthrene degradative enzymes in strain 20006FA and enabled an elucidation of the metabolic pathway utilized by the bacterium. To our knowledge the present work represents the first investigation of genomic, proteomic, and physiological studies of a PAH-degrading Sphingomonas strain.

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Construction of PAH-degrading mixed microbial consortia by induced selection in soil

Cited by 63 publications

References 29 publications

Rubber gloves biodegradation by a consortium, mixed culture and pure culture isolated from soil samples

Rubber gloves biodegradation by a consortium, mixed culture and pure culture isolated from soil samples

Degradation of hydrocarbon by isolated fungal species with laccase activity

Insights into the genome and proteome of Sphingomonas paucimobilis strain 20006FA involved in the regulation of polycyclic aromatic hydrocarbon degradation

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