Degradation of hydrocarbon by isolated fungal species with laccase activity

Abstract

Hydrocarbon of crude oil and other oil products such as gasoline comprise aromatic, aliphatic and alicylic components pollute water bodies are hazardous. Microbial degradation of these oil spills are an efficient way. Degrading oil contamination by microorganism is the theme of this paper. To study degrading efficiency of crude oil hydrocarbon by fungi. Fungal species were isolated from crude oil soaked soil samples and oil contaminated soil samples from commercial oil industry. Isolated fungal species were cultured on nutrient media such as potato dextrose agar (PDA) and czapekdox agar at 37 ºC for 7 day. Further, fungal species were identified by 18 S rRNA sequencing. The potential of fungi to degrade hydrocarbon was investigated through laccase and peroxidase. To examine the ability to degrade oil, the fungal species were screened on Czapekdox agar and broth medium containing petroleum derivatives. Laccase activity was performed by ABTS (2, 2̍-azinobis 3ethyl-benzothiazoline-6-sulphonate) and bromophenol. They were used as substrate for screening laccase activity. Among the isolated eight fungal species, isolate FGS-4 showed better biodegradation ability and subsequent 18 S rRNA revealed that isolate FGS-4 belongs to Aspergillus tubigensis. Production of laccase enzyme was confirmed. The results of gas chromatography analysis showed that hydrocarbon was biodegraded by the isolated Aspergillus tubigensis. Biodegradation of hydrocarbon by Aspergillus species would create an impact on various waste treatment methods. Eco-friendly degradation of hydrocarbons could be achieved by Aspergillus species.

Cometabolic biodegradation of chlorinated ethenes with methanotrophs in anaerobic/aerobic simulated aquifer

“…TCE, cDCE and VC can be cometabolized and degraded by non-specific oxidation enzymes of aliphatic and aromatic degrading bacteria with methane, propane, toluene, and phenol as primary substrates (Elango et al, 2011;Findlay et al, 2016;Li et al, 2014). The cost of this type of bioremediation technology is competitive with traditional chemistry/physics processes (Frascari et al, 2015), and biodegradation is one of the safer and environmentally friendly methods (Gnanasekaran et al, 2019).However, in most cases, due to the provisions of laws and regulations, there are still many doubts on adding the aforementioned substrates into the groundwater, since they may cause secondary pollution, which provides a challenge to in situ bioremediation. Methanotrophs are widely distributed in nature, as long as there is an environment where methane and air coexist.…”