Construction of HSV-1 BACs and Their Use for Packaging of HSV-1-Based Amplicon Vectors

Heister, Thomas; Vögtlin, Andrea; Müller, Lars; Heid, Irma; Fraefel, Cornel

doi:10.1385/1-59259-753-x:241

Cited by 1 publication

(1 citation statement)

References 15 publications

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“…Virulent wild-type strains cause respiratory disease, abortion storms and neurological disorders in equines (Telford et al, 1992). Cloning of herpesviruses as bacterial artificial chromosomes (BACs) has proven a reliable and efficient method to manipulate herpesviral genomes (Borst et al, 2004;Brune et al, 1999Brune et al, , 2000Heister et al, 2004;Kawaguchi & Tanaka, 2004;McGregor & Schleiss, 2001;Messerle et al, 1997;Osterrieder et al, 2003;Rosas et al, 2006;Saeki, 2000;Wagner et al, 1999Wagner et al, , 2002. Several strains of EHV-1, including the vaccine strain RacH, have been cloned as infectious BACs by the introduction of a mini-F plasmid into the viral genome .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the vaccine potential of an equine herpesvirus type 1 vector expressing bovine viral diarrhea virus structural proteins

Rosas

König

Beer

et al. 2007

Journal of General Virology

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Bovine viral diarrhea virus (BVDV) is an economically important pathogen of cattle that is maintained in the population by persistently infected animals. Virus infection may result in reproductive failure, respiratory disease and diarrhoea in naïve, susceptible bovines. Here, the construction and characterization of a novel vectored vaccine, which is based on the incorporation of genes encoding BVDV structural proteins (C, Erns, E1, E2) into a bacterial artificial chromosome of the equine herpesvirus type 1 (EHV-1) vaccine strain RacH, are reported. The reconstituted vectored virus, rH_BVDV, expressed BVDV structural proteins efficiently and was indistinguishable from parental vector virus with respect to growth properties in cultured cells. Intramuscular immunization of seronegative cattle with rH_BVDV resulted in induction of BVDV-specific serum neutralizing and ELISA antibodies. Upon experimental challenge infection of immunized calves with the heterologous BVDV strain Ib SE5508, a strong anamnestic boost of the neutralizing-antibody response was observed in all vaccinated animals. Immunized animals presented with reduced viraemia levels and decreased nasal virus shedding, and maintained higher leukocyte counts than mock-vaccinated controls.

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