Construction of full-length infectious cDNA clones of two Korean isolates of turnip mosaic virus breaking resistance in Brassica napus

Song, Zheng-Xing; Seo, Eun-Young; Hu, Wen-Xing; Jeong, Jong‐Hyeon; Moon, Jae Sun; Kim, Kanghee; Eom, Won-Seob; Cho, In-Sook; Lim, Hyoun–Sub

doi:10.1007/s00705-022-05381-2

Cited by 2 publications

(2 citation statements)

References 32 publications

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“…B. rapa is susceptible to infection by plant viruses, especially to TuMV. Several genes that confer resistance against TuMV have been mapped and cloned in Brassica …”

Section: Proteomics and Biotic Stressmentioning

confidence: 99%

“…Several genes that confer resistance against TuMV have been mapped and cloned in Brassica. 59 Liu et al, 60 studied the genes and complexes participating in the process of transportation and spread of virus particles through phloem, xylem, and plasmodesmata in B. rapa. They identified 6043 distinct proteins, many of which are involved in several complex regulatory pathways in the long-distance movement of TuMV.…”

Section: Virusesmentioning

confidence: 99%

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Understanding the Proteomes of Plant Development and Stress Responses in Brassica Crops

et al. 2023

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Brassica crops have great economic value due to their rich nutritional content and are therefore grown worldwide as oilseeds, vegetables, and condiments. Deciphering the molecular mechanisms associated with the advantageous phenotype is the major objective of various Brassica improvement programs. As large technological advancements have been achieved in the past decade, the methods to understand molecular mechanisms underlying the traits of interest have also taken a sharp upturn in plant breeding practices. Proteomics has emerged as one of the preferred choices nowadays along with genomics and other molecular approaches, as proteins are the ultimate effector molecules responsible for phenotypic changes in living systems, and allow plants to resist variable environmental stresses. In the last two decades, rapid progress has been made in the field of proteomics research in Brassica crops, but a comprehensive review that collates the different studies is lacking. This review provides an inclusive summary of different proteomic studies undertaken in Brassica crops for cytoplasmic male sterility, oil content, and proteomics of floral organs and seeds, under different biotic and abiotic stresses including post-translational modifications of proteins. This comprehensive review will help in understanding the role of different proteins in controlling plant phenotypes, and provides information for initiating future studies on Brassica breeding and improvement programs.

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“…B. rapa is susceptible to infection by plant viruses, especially to TuMV. Several genes that confer resistance against TuMV have been mapped and cloned in Brassica …”

Section: Proteomics and Biotic Stressmentioning

confidence: 99%

Section: Virusesmentioning

confidence: 99%

Understanding the Proteomes of Plant Development and Stress Responses in Brassica Crops

et al. 2023

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Evaluation of a Series of Turnip Mosaic Virus Chimeric Clones Reveals Two Amino Acid Sites Critical for Systemic Infection in Chinese Cabbage

Song

Seo

et al. 2023

Phytopathology®

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Two infectious clones of turnip mosaic virus (TuMV), pKBC-1 and pKBC-8, with differential infectivity in Chinese cabbage (Brassica rapa subsp. pekinensis) were obtained. Both infected Nicotiana benthamiana systemically, inducing similar symptoms, whereas only virus KBC-8 infected Chinese cabbage systemically. To identify the determinants affecting infectivity on Chinese cabbage, chimeric clones were constructed by restriction fragment exchange between the parental clones, and tested on several Chinese cabbage cultivars. Chimeric clones p1N8C and p8N1C demonstrated that the C-terminal portion of the polyprotein determines systemic infection of Chinese cabbage despite only three amino acid differences in this region, in the cylindrical inclusion (CI), viral protein genome-linked (VPg) and coat protein (CP). A second pair of hybrid constructs, pHindIII-1N8C and pHindIII-8N1C, failed to infect cultivars CR Victory and Jinseonnorang systemically, yet pHindIII-1N8C caused hypersensitive response (HR)-like lesions on inoculated leaves of these cultivars, and could systemically infect cultivars CR Chusarang and Jeongsang; this suggests that R genes effective against TuMV may exist in the first two cultivars but not the latter two. Constructs with single amino acid changes in both VPg (K2045E) and CP (Y3095H) failed to infect Chinese cabbage, implying that at least one of these two amino acid substitutions is essential for successful infection on Chinese cabbage. Successful infection by mutant KBC-8-CP-H and delayed infection with mutant HJY1-VPg-E following mutation or reversion suggested that VPg (2045K) is the residue required for infection of Chinese cabbage, and involved in the interaction between VPg and eukaryotic initiation factor eIF(iso)4E, confirmed by yeast two-hybrid assay.

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Construction of full-length infectious cDNA clones of two Korean isolates of turnip mosaic virus breaking resistance in Brassica napus

Cited by 2 publications

References 32 publications

Understanding the Proteomes of Plant Development and Stress Responses in Brassica Crops

Understanding the Proteomes of Plant Development and Stress Responses in Brassica Crops

Evaluation of a Series of Turnip Mosaic Virus Chimeric Clones Reveals Two Amino Acid Sites Critical for Systemic Infection in Chinese Cabbage

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