Construction of complete Tupaia belangeri transcriptome database by whole-genome and comprehensive RNA sequencing

Sanada, Takahiro; Tsukiyama-Kohara, Kyoko; Shin-I, Tadasu; Yamamoto, Naoki; Kayesh, Mohammad Enamul Hoque; Yamane, Daisuke; Takano, Junichiro; Shiogama, Yumiko; Yasutomi, Yasuhiro; Ikeo, Kazuho; Gojobori, Takashi; Mizokami, Masashi; Kohara, Michinori

doi:10.1038/s41598-019-48867-x

Cited by 19 publications

(18 citation statements)

References 37 publications

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“…Tree shrews (Tupaia belangeri chinensis), belongs to the family Tupaiidae, genus Tupaia, are rat-sized mammal that live mainly in deciduous, evergreen and secondary forests in tropical and subtropical regions (1). Whole-genome sequencing analysis has revealed that tree shrews are similar to humans with regard to histology, physiology, biochemistry, and nervous (brain function), metabolic and immune systems (2)(3)(4). The tree shrew is small, has a short reproductive cycle, is easy to perform experiments on, is inexpensive to feed, and has other favorable characteristics, such as susceptible to a wide range of human pathogenic viruses (5).…”

Section: Introductionmentioning

confidence: 99%

Histological analysis of spermatogenesis and the germ cell seasonal development within the testis of domesticated tree shrews (Tupaia belangeri chinensis)

Tang

Yang

et al. 2022

Folia Morphol

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This study aimed to address the lack of information on the male germ cell seasonal development of domesticated tree shrews (Tupaia belangeri chinensis). Testicular tissues were collected from 60 tree shrews (n=5 per month). The ultrastructures of the testes and spermatids were examined via transmission electron microscopy. Apoptosis of spermatogenic cells was measured through terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. The expression of proliferation factors, namely, proliferating cell nuclear antigen (PCNA) and Ki67, in testicular tissues was assayed through immunohistochemistry. Spermatids ultrastructure showed seasonal differences, and spermatogenesis was relatively active in June and July and relatively stagnant from October to November. The percentage of TUNEL-positive germ cells was less during October and November, while greater in July than other phases. The number of PCNA-nucleus-positive germ cells was most in June and July, but with cytoplasm staining from October to November. Ki67 presented positive expression in the testes from April to September, with highest expression in June, but with no expression from October to March. In summary, there are seasonal differences in tissue morphology related to spermatogenesis in domesticated tree shrews. PCNA expression and Ki67 expression are good indicators of seasonal differences in male germ cells.

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Section: Introductionmentioning

confidence: 99%

Histological analysis of spermatogenesis and the germ cell seasonal development within the testis of domesticated tree shrews (Tupaia belangeri chinensis)

Tang

Yang

et al. 2022

Folia Morphol

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“…The antiviral gene RIG-I (retinoic acid inducible gene I) was also lost during evolution (14). As a close relative of non-human primates, the whole genome of the tree shrew has been sequenced, and the results show that the nervous, immune and metabolic systems of the tree shrew are similar to those of humans (15)(16)(17). The relevant genomic data can be queried in the tree shrew database (TreeshrewDB) (http:// www.treeshrewdb.org) (18).…”

Section: Introductionmentioning

confidence: 99%

The Tree Shrew as a Model for Cancer Research

Peng

Zhong

et al. 2021

Front. Oncol.

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Animal disease models are necessary in medical research, and an appropriate animal model is of great importance for studies about the prevention or treatment of cancer. The most important thing in the selection of animal models is to consider the similarity between animals and humans. The tree shrew (Tupaia belangeri) is a squirrel-like mammal which placed in the order Scandentia. Whole-genome sequencing has revealed that tree shrews are extremely similar to primate and humans than to rodents, with many highly conserved genes, which makes the data from studies that use tree shrews as models more convincing and the research outcomes more easily translatable. In tumor research, tree shrews are often used as animal models for hepatic and mammary cancers. As research has progressed, other types of tree shrew tumor models have been developed and exhibit clinical manifestations similar to those of humans. Combining the advantages of both rodents and primates, the tree shrew is expected to be the most powerful animal model for studying tumors.

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“…Tupaia belangeri is a small non-primate mammal belonging to the family Tupaiidae, which has a body weight of approximately 150 g [4]. In comparison with rodents, the genome sequence of tree shrew shows a higher homology with that of primates [4][5][6]. The tree shrew shows susceptibility to hepatitis viruses infections (hepatitis C virus [7,8], hepatitis B virus [9,10]) and can be used for the evaluation of anti-depressant drugs [11].…”

Section: Introductionmentioning

confidence: 99%

Pathological and genetic aspects of spontaneous mammary gland tumor in Tupaia belangeri (tree shrew)

et al. 2020

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Mammary gland cancer is the most common cancer occurring in women globally. Incidences of this cancer in Japan are on the increase. Annually, more than 70,000 new cases are recorded in Japan and about 1.7 million in the world. Many cases are still difficult to cure completely, and animal models are required for the characterization of the biology, therapeutic strategy, and preventive measures for spontaneous mammary tumor. The mouse model used currently has some limitations owing to structural differences between mouse and human mammary glands. Tupaia belangeri (tree shrew), which belongs to the Tupaiidae family, shows relatively high genetic homology and structural similarity to human mammary glands. Here, we characterized the spontaneous mammary tumors in 61 female tree shrews of different ages. The incidence rate was 24.6% (15/61), and the rate of simultaneous or metachronous multiplex tumors was 60% (9/15). From the incidence pattern, some cases seemed to be of familial mammary gland tumor, as the offspring of female tree shrews No. 3 and 9 and male tree shrew No. 11 showed a high incidence rate, of 73.3% (11/15). Average incidence age for tumor development was 2 years and 3 months, and the earliest was 10 months. Histochemical analysis indicated that spontaneous mammary gland tumors in the tree shrew show the features of intraductal papillary adenomas (22 cases), except 2 tubulopapillary carcinoma cases (No. 75 and 131). All the cases were positive for the progesterone receptor, whereas 91.3% were positive for the estrogen receptor, and 4.3% were HER-2 positive. We have also confirmed the expression of nectin-4 in some mammary tumor cells. Additionally, we subjected tree shrews to cytodiagnosis or X-ray CT. Thus, the findings of this study highlight the potential of the tree shrew as a valuable new animal model for mammary gland tumor study.

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Construction of complete Tupaia belangeri transcriptome database by whole-genome and comprehensive RNA sequencing

Cited by 19 publications

References 37 publications

Histological analysis of spermatogenesis and the germ cell seasonal development within the testis of domesticated tree shrews (Tupaia belangeri chinensis)

Histological analysis of spermatogenesis and the germ cell seasonal development within the testis of domesticated tree shrews (Tupaia belangeri chinensis)

The Tree Shrew as a Model for Cancer Research

Pathological and genetic aspects of spontaneous mammary gland tumor in Tupaia belangeri (tree shrew)

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