Construction of chiral ligand exchange capillary electrochromatography for d , l -amino acids enantioseparation and its application in glutaminase kinetics study

Zhao, Liping; Qiao, Juan; Zhang, Ke; Li, Dan; Zhang, Hongyi; Qi, Li

doi:10.1016/j.chroma.2018.03.031

Cited by 14 publications

(11 citation statements)

References 30 publications

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“…In recent years, there were many novel homo‐polymers or copolymers synthesized in order to meet the needs for the separation of different analytes . Liu and co‐workers reported an example of partially filled PLOT capillary column with immobilized pH gradient (PLOT‐IPG) for OT‐CEC analysis of amino acids and proteins.…”

Section: Open‐tubular Capillary Electrochromatographymentioning

confidence: 99%

“…Zhao et al. developed a block copolymer poly(maleic anhydridestyrene‐methacryloyl‐l‐arginine methyl ester) coated capillary. The prepared OT‐column could be used for the enantiomer separation of d , l ‐amino acids on the base of synergistic ligands effect of the immobilized polymethylacrylic acid in the coating and free l ‐arginine in buffer solution.…”

Section: Open‐tubular Capillary Electrochromatographymentioning

confidence: 99%

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Recent developments of monolithic and open‐tubular capillary electrochromatography (2017–2019)

Yin

Zhang

et al. 2020

J of Separation Science

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Capillary electrochromatography, which combined the high selectivity of highperformance liquid chromatography and the high separation efficiency of capillary electrophoresis, is an attractive separation tool. In this review, the developments on monolithic and open tubular capillary electrochromatography during 2017 to August 2019 are summarized. Considering the development of novel stationary phases is the most active research field in capillary electrochromatography, monolithic capillary electrochromatography is classified according to the polymer-based and hybrid monolithic columns, while open-tubular capillary electrochromatography is categorized by cyclodextrin, silica, polymer, nanomaterials, microporous materials, and biomaterials-based open tubular columns. K E Y W O R D S

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Section: Open‐tubular Capillary Electrochromatographymentioning

confidence: 99%

Section: Open‐tubular Capillary Electrochromatographymentioning

confidence: 99%

Recent developments of monolithic and open‐tubular capillary electrochromatography (2017–2019)

Yin

Zhang

et al. 2020

J of Separation Science

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“…Qi’s group [41] developed a chiral ligand exchange stationary phase poly(maleic anhydride-styrene-methacryloyl- l -arginine methyl ester) for the enantioseparation of d , l -amino acids. The chiral stationary phase showed excellent enantioseparation performance.…”

Section: Open-tubular Columnmentioning

confidence: 99%

Advances in capillary electro-chromatography

Mao

Chen

2019

Journal of Pharmaceutical Analysis

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Capillary electrochromatography (CEC) is a micro-scale separation technique which is a hybrid between capillary electrophoresis (CE) and liquid chromatography (LC). CEC can be performed in packed, monolithic and open-tubular columns. In recent three years (from 2016 to 2018), enormous attention for CEC has been the development of novel stationary phases. This review mainly covers the development of novel stationary phases for open-tubular and monolithic columns. In particular, some biomaterials attracted increasing interest. There are no significant breakthroughs in technology and principles in CEC. The typical CEC applications, especially chiral separations are described.

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“…At present, chromatography and electrophoresis (CE) methods are mostly employed for the detection of SA residues, about 75% of the total [4]. Capillary electrochromatography (CEC) is considered to combine the advantages of the high separation efficiency of CE and the selectivity supplied by liquid chromatography [5,6]. It is important to realize that the CEC system operates with small sample quantities (nanolitre amounts) and sample vials used for the introduction of the sample only require microlitre quantities (typically greater than or equal to 50 µl), which is quite attractive for precious biological samples [7].…”

Section: Introductionmentioning

confidence: 99%

Determination of sulfamerazine in aquatic products by molecularly imprinted capillary electrochromatography

Qin

Jin²,

Gao

et al. 2019

R. Soc. open sci.

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A molecularly imprinted monolith was prepared and evaluated for the special selective separation of sulfamerazine (SMR) by capillary electrochromatography (CEC). The single-step in situ polymerization method was applied through thermally immobilized vinyl groups of itaconic acid and a derivatization capillary column using SMR as the template. The monolith with optimal selectivity and permeability was performed at 45°C for 7 h according to the molar ratios of 1 : 4 : 10 (template/functional monomer/cross-linker). Under the optimized separation conditions of 75% acetonitrile in 20 mM phosphate buffer with pH 5.0, 15 kV applied voltage and 20°C column temperature, the imprinted monolith showed strong recognition ability for SMR and high column performance. Finally, the molecularly imprinted monolith coupled with the CEC method was successfully developed for the quantification of SMR in aquatic products, which was properly validated by a good linear relationship, recoveries and limit of detection. The coupling technique of the molecularly imprinted technology and CEC achieved pre-treatment enrichment and separation analysis in only one miniaturized chromatographic column.

show abstract

Construction of chiral ligand exchange capillary electrochromatography for d , l -amino acids enantioseparation and its application in glutaminase kinetics study

Cited by 14 publications

References 30 publications

Recent developments of monolithic and open‐tubular capillary electrochromatography (2017–2019)

Recent developments of monolithic and open‐tubular capillary electrochromatography (2017–2019)

Advances in capillary electro-chromatography

Determination of sulfamerazine in aquatic products by molecularly imprinted capillary electrochromatography

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