Construction of cDNA subtractive library from pearl oyster (Pinctada fucata Gould) with red color shell by SSH

Guan, Yunyan; Huang, Liangmin; He, Maoxian

doi:10.1007/s00343-011-0200-x

Cited by 11 publications

(4 citation statements)

References 32 publications

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“…Its production increases with increasing calcium concentration and means that a higher biomineralization rate is occurring [31] . She-7-F10 shared high levels of identity with shell matrix structural proteins and was thought to be involved in the process of shell biomineralization [32] . Hsp70 comprise a group of highly conserved proteins that have general protective function in all living organisms [33] .…”

Section: Introductionmentioning

confidence: 99%

Seawater Acidification and Elevated Temperature Affect Gene Expression Patterns of the Pearl Oyster Pinctada fucata

Liu

Huang²,

Lin³

et al. 2012

PLoS ONE

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Oceanic uptake of anthropogenic carbon dioxide results in decrease in seawater pH and increase in temperature. In this study, we demonstrated the synergistic effects of elevated seawater temperature and declined seawater pH on gene expression patterns of aspein, calmodulin, nacrein, she-7-F10 and hsp70 in the pearl oyster Pinctada fucata. Under ‘business-as-usual’ scenarios, four treatments were examined: (1) ambient pH (8.10) and ambient temperature (27°C) (control condition), (2) ambient pH and elevated temperature (+3°C), (3) declined pH (7.70) and ambient temperature, (4) declined pH and elevated temperature. The results showed that under warming and acidic seawater conditions, expression of aspein and calmodulin showed no significant differences among different time point in condition 8.10 T. But the levels of aspein and calmodulin in conditions 8.10 T+3, 7.70 T and 7.70 T+3, and levels of nacrein, she-7-F10 in all the four treatments changed significantly. Low pH and pH×temperature interaction influenced the expression of aspein and calmodulin significantly after hours 48 and 96. Significant effects of low pH and pH×temperature interaction on the expression of nacrein were observed at hour 96. The expression level of she-7-F10 was affected significantly by pH after hours 48 and 96. The expression of hsp70 was significantly affected by temperature, pH, temperature×pH interaction at hour 6, and by temperature×pH interaction at hour 24. This study suggested that declined pH and pH×temperature interaction induced down regulation of calcification related genes, and the interaction between declined seawater pH and elevated temperature caused up regulation of hsp70 in P. facata. These results demonstrate that the declined seawater pH and elevated temperature will impact the physiological process, and potentially the adaptability of P. fucata to future warming and acidified ocean.

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Section: Introductionmentioning

confidence: 99%

Seawater Acidification and Elevated Temperature Affect Gene Expression Patterns of the Pearl Oyster Pinctada fucata

Liu

Huang²,

Lin³

et al. 2012

PLoS ONE

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“…The purple shells are stably inherited and do not change with the environment, which is not investigated in this study. Research has focussed on the molecular genetics of shell colour traits in bivalves, such as the bay scallop Argopecten irradians (Qin et al, 2007), Pacific lion‐paw scallop Nodipecten subnodosus (Petersen et al, 2012), noble scallop Chlamys nobilis (Yuan et al, 2012; Zheng et al, 2013), Japanese scallop Patinopecten yessoensis (Ding et al, 2015), Pacific oyster Crassostrea gigas (Brake et al, 2004), freshwater triangle pearl mussels Hyriopsis cumingii (Bai et al, 2013; Wen et al, 2013), pearl oyster Pinctada fucata (Guan et al, 2011), and the clams C. sinensis , Macoma balthica and Meretrix meretrix (Luttikhuizen & Drent, 2008; Sun et al, 2015; Yue et al, 2015). However, the nonhereditary shell colour trait of bivalves, especially in the black shell of C. sinensis , has rarely been studied.…”

Section: Introductionmentioning

confidence: 99%

Cloning and characterization of microphthalmia‐associated transcription factor‐like gene provide insights intoCyclina sinensisclam shell melanin deposition

Qiu¹,

Zhang²,

Wu³

et al. 2021

Aquaculture Research

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The reversible black shell colour change in the clam Cyclina sinensis functions as an adaptation to different environmental conditions (within or outside of muddy sediment) due to changes in the melanin redox state in the shell. The microphthalmia‐associated transcription factor (MITF) is a melanocyte‐specific transcription factor that is involved in the differentiation, proliferation and survival of melanocytes. In this study, the full‐length cDNA sequence of the mitf‐like gene was obtained from C. sinensis using RACE PCR. The mitf‐like cDNA is 1725 bp in length, containing a 47 bp 5′ untranslated region (UTR), a 1398 bp open reading frame and a 280 bp 5′ untranslated region. The mitf‐like gene contains seven exons separated by six introns, and it encodes a putative protein of 465 amino acids with a highly conserved domain of MITF containing a basic helix‐loop‐helix leucine zipper (bHLH‐LZ). The phylogenetic analysis of amino acid sequences using neighbour‐joining and maximum likelihood methods revealed that C. sinensis is closely related to Meretrix petechialis. Additionally, the mRNA expression of mitf‐like and melanin was high in the outer margin of the mantle, suggesting that the mitf‐like might play an important role in melanin synthesis in the mantle by regulating the cell cycle and survival of melanocytes; however, its role in C. sinensis shell melanin deposition requires further investigation.

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“…Two cDNA suppression subtractive libraries constructed for red-shelled and non-red-shelled Pfu. revealed that genes encoding shematrin, mantle protein, and nacrein are related to shell colour [5]. Gong [6] found that an increased concentration of calcium ions can enhance nacrein secretion.…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptomic and proteomic analysis of yellow shell and black shell pearl oysters, Pinctada fucata martensii

Huang

Shi

et al. 2019

BMC Genomics

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Background The pearl oyster Pinctada fucata martensii ( Pfu. ), widely cultured in the South China Sea, is a precious source of sea pearls and calcifying materials. A yellow shell variety of Pfu. was obtained after years of artificial breeding. To identify differentially expressed genes between yellow shell and normal black shell pearl oysters, we performed transcriptomic sequencing and proteomic analyses using mantle edge tissues. Results A total of 56,969 unigenes were obtained from transcriptomic, of which 21,610 were annotated, including 385 annotated significant up-regulated genes and 227 significant down-regulated genes in yellow shell oysters (| log 2 (fold change) | ≥2 and false discovery rate < 0.001). Tyrosine metabolism, calcium signalling pathway, phototransduction, melanogenesis pathways and rhodopsin related Gene Ontology (GO) terms were enriched with significant differentially expressed genes (DEGs) in transcriptomic. Proteomic sequencing identified 1769 proteins, of which 51 were significantly differentially expressed in yellow shell oysters. Calmodulin, N66 matrix protein, nacre protein and Kazal-type serine protease inhibitor were up-regulated in yellow shell oysters at both mRNA and protein levels, while glycine-rich protein shematrin-2 , mantle gene 4, and sulphide: quinone oxidoreductase were down-regulated at two omics levels. Particularly, calmodulin, nacre protein N16.3, mantle gene 4, sulphide: quinone oxidoreductase, tyrosinase-like protein 3, cytochrome P450 3A were confirmed by quantitative real-time PCR. Yellow shell oysters possessed higher total carotenoid content (TCC) compared than black shell oyster based on spectrophotography. Conclusions The yellow phenotype of pearl oysters, characterised by higher total carotenoids content, may reflect differences in retinal and rhodopsin metabolism, melanogenesis, calcium signalling pathway and biomineralisation. These results provide insights for exploring the relationships between calcium regulation, biomineralisation and yellow shell colour pigmentation. Electronic supplementary material The online version of this article (10.1186/s12864-019-5807-x) contains supplementary material, which is available to authorized users.

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Construction of cDNA subtractive library from pearl oyster (Pinctada fucata Gould) with red color shell by SSH

Cited by 11 publications

References 32 publications

Seawater Acidification and Elevated Temperature Affect Gene Expression Patterns of the Pearl Oyster Pinctada fucata

Seawater Acidification and Elevated Temperature Affect Gene Expression Patterns of the Pearl Oyster Pinctada fucata

Cloning and characterization of microphthalmia‐associated transcription factor‐like gene provide insights intoCyclina sinensisclam shell melanin deposition

Comparative transcriptomic and proteomic analysis of yellow shell and black shell pearl oysters, Pinctada fucata martensii

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