Construction of biocompatible bovine serum albumin nanoparticles composed of nano graphene oxide and AIEgen for dual-mode phototherapy bacteriostatic and bacterial tracking

Abstract

Background Efficient and highly controllable antibacterial effect, as well as good biocompatibility are required for antibacterial materials to overcome multi-drug resistance in bacteria. Herein, nano graphene oxide (NGO)-based near-infrared (NIR) photothermal antibacterial materials was schemed to complex with biocompatible bovine serum albumin (BSA) and aggregation-induced emission fluorogen (AIEgen) with daylight-stimulated ROS-producing property for dual-mode phototherapy in the treatment of antibiotic resistance bacteria. Results Upon co-irradiation of daylight and NIR laser, NGO-BSA-AIE nanoparticles (NPs) showed superiorly antibacterial effect (more than 99%) both against amoxicillin (AMO)-resistant Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) by comparison with sing-model phototherapy. Meanwhile, the NGO-BSA-AIE NPs displayed prominent stability and excellently controllable biocompatibility. More importantly, under daylight irradiation, the AIEgen not only produced plentiful ROS for killing bacteria, but also presented fluorescence image for tracking bacteria. Conclusions Hence, the designed system provided tempting strategy of employing light as impetus for tracking bacterial distribution and photothermal/photodynamic synergistic treatment of antibiotic resistance antibacterial.

The fast-growing field of photo-driven theranostics based on aggregation-induced emission

“…23a). 280 Due to its strong hydrophobicity, the AIE PS could be facilely entrapped into amphiphilic nano-graphene oxide and BSA. The integration of biocompatible BSA was schemed to improve the water solubility and physiological stability of the nanocomposite.…”

Two-Dimensional Nanomaterials for Photoinduced Antibacterial Applications

“…This allowed them to use low concentration of GO, which in turn showed lesser human dermal fibroblasts but retained its antibacterial activity. Zhang et al reported a biocompatible NGO-BSA-AIE nanoparticle to show superior antibacterial effect via ROS generation along with fluorescent bio-tracking of the bacterial activity . AIE produces fluorescence under white light of 10 mW/cm 2 and conjugation with BSA via hydrophobic interactions ensures its stability in aqueous solutions.…”

“…Zhang et al reported a biocompatible NGO-BSA-AIE nanoparticle to show superior antibacterial effect via ROS generation along with fluorescent bio-tracking of the bacterial activity. 69 AIE produces fluorescence under white light of 10 mW/cm 2 and conjugation with BSA via hydrophobic interactions ensures its stability in aqueous solutions. Irradiation with 795 nm laser at 2.5 W/cm 2 for 5 min and daylight for 1 h resulted in dual-mode phototherapy.…”

AIEgen-loaded nanofibrous membrane as photodynamic/photothermal antimicrobial surface for sunlight-triggered bioprotection

“…In addition, the existence of molecular rotors in the AIEgen skeleton could facilitate excited-state energy dissipation via non-radiative decay and thus promote photothermal conversion [ 37 , 38 ]. Combining these unique features, a few AIEgens were developed for synergistic photodynamic and photothermal treatment by making full use of both ROS and heat [ [39] , [40] , [41] ]. However, those researches mostly focused on the bacteria killing in solution or infection treatment in vivo, researches regarding the fabrication of AIEgen-loaded hybrid material as antimicrobial surfaces are still rare.…”