Construction of antibacterial nano-silver embedded bioactive hydrogel to repair infectious skin defects

Abstract

Background Hydrogels loaded with antimicrobial agents have been widely used for treating infected wound defects. However, hydrogels derived from a porcine dermal extracellular matrix (PADM), containing silver nanoparticles (AgNPs), have not yet been studied. Therefore, we investigated the therapeutic effect of an AgNP-impregnated PADM (AgNP–PADM) hydrogel on the treatment of infected wounds. Methods An AgNP–PADM hydrogel was synthesized by embedding AgNPs into a PADM hydrogel. We examined the porosity, moisture retention, degradation, antibacterial properties, cytotoxicity, antioxidant properties, and ability of the PADM and AgNP–PADM hydrogels to treat infected wounds in animals. Results The PADM and AgNP–PADM hydrogels were pH sensitive, which made them flow dynamically and solidify under acidic and neutral conditions, respectively. The hydrogels also exhibited porous network structures, satisfactory moisture retention, and slow degradation. Additionally, the AgNP–PADM hydrogel showed a slow and sustained release of AgNPs for at least 7 days without the particle size changing. Thus, the AgNPs exhibited adequate antibacterial ability, negligible toxicity, and antioxidant properties in vitro. Moreover, the AgNP–PADM hydrogel promoted angiogenesis and healed infected skin defects in vivo. Conclusions The AgNP–PADM hydrogel is a promising bioderived antibacterial material for clinical application to infected wound dressings.

Three-Dimensional Evaluation of the Cytotoxicity and Antibacterial Properties of Alpha Lipoic Acid-Capped Silver Nanoparticle Constructs for Oral Applications

“…Similarly, the particles’ diameter found in GelMA matches the shape and size range of the originally characterised AgNPs pre-encapsulation. Similar results were reported where TEM images revealed that the 5 nm AgNP sizes were negligibly different before the AgNPs had been added to a porcine derived hydrogel; however, aggregation was detected with a bigger particle size of 50 nm [ 46 ].…”

High-Efficiency Photodynamic Control of Antibiotic-Resistant Bacteria and Antibiotic-Resistant Genes in Wastewater Using Hydrogel-Decorated Porous Polyurethane Sponges with Metal Nanoparticles

“…AgNP-loaded CS-AA hydrogels revealed that the different sizes were compared with the pure AgNPs. As shown in Figure S1D, AgNPs in the CS-AA/AgNPs composite were found to have an average size of 60 nm ranging from 10 to 80 nm . These observations are mainly to the strong interaction between the AgNPs and CS-AA hydrogels.…”

“…As shown in Figure S1D, AgNPs in the CS-AA/AgNPs composite were found to have an average size of 60 nm ranging from 10 to 80 nm. 49 These observations are mainly to the strong interaction between the AgNPs and CS-AA hydrogels. It was expected that a homogeneous distribution of AgNPs would be achieved throughout the CS-AA hydrogels, although there was a quick reduction of the silver ions into the AgNPs.…”

Nanomaterials-Functionalized Hydrogels for the Treatment of Cutaneous Wounds

“…Ag NPs porcine dermal decellularized extracellular matrix Ag NPs destroyed the structure of bacteria. [206] reduced graphene oxide (rGO) DA modified HA (DA-HA) High temperature (above 50°C) could kill bacteria through destroying some enzymes and proteins.…”