Construction of an electrochemical sensing platform based on platinum nanoparticles supported on carbon for tetracycline determination

Abstract

a b s t r a c tIn this paper, we report the construction of an electrochemical sensing platform based on platinum nanoparticles supported on carbon (PtNPs/C) for the determination of the antibiotic tetracycline (TTC). Initially, the PtNPs/C was synthesized and characterized by powder X-ray diffraction spectroscopy and transmission electron microscopy. The effect of experimental parameters on the electrochemical behavior of the PtNPs/C coated glassy carbon electrode (PtNPs/C/GCE), including the amount of PtNPs and electroactive area, scan rate and pH, were evaluated. The electrooxidation of TTC on the PtNPs/C/GCE was investigated by cyclic voltammetry and differential pulse voltammetry, with the determination of TTC electrooxidation mechanism. Under optimum experimental conditions, the linear calibration range, detection limit, quantification and sensitivity of the PtNPs/C/GCE were 9.99-44.01 mol L −1 , 4.28 mol L −1 , 14.3 mol L −1 and 3.32 A L mol −1 cm −2 , respectively. Finally, the proposed electrochemical sensing platform was successfully applied to determine low TTC concentrations in urine samples, suggesting its great applicability in clinical analysis and quality control.

Uncured Polydimethylsiloxane as Binder Agent for Carbon Paste Electrodes: Application to the Quantification of Propranolol

“…The active electrochemical areas of PDMS and Nujol ® -CPE were determined by chronoamperometry using [Fe(CN) 6 ] 4as electroactive specie. 57,58 Cottrell's equation was employed with the following parameters: the number of electrons transferred = 1; the Faraday constant = 96,485 C mol -1 ; concentration of [Fe(CN) 6 ] 4-= 1.0 × 10 -5 mol cm -3 and average diffusion coefficient of [Fe(CN) 6 ] 4in 1.0 mol L -1 KCl = 6.58 × 10 -6 cm 2 s -1 . 59 The chronoamperometric curves and the respective I vs. t -1/2 plots are presented in Figure S3 (SI section).…”

Voltammetric determination of Sudan I in food samples based on platinum nanoparticles decorated on graphene-β-cyclodextrin modified electrode

“…In addition, the decoration of metal nanoparticles on the graphene/β-CD composite could further enhanced the surface area and active sites of the graphene/β-CD composite. Compared with other metal and metal alloy nanoparticles, platinum nanoparticles (PtNPs) has large surface area and high stability [21,22]. Hence, in the present study, for the first time we have decorated PtNPs on graphene/β-CD composite and used as a sensitive probe for low-level electrochemical detection of Sudan I.…”

Electrochemically Reduced Graphene Oxide-Based Screen-Printed Electrodes for Total Tetracycline Determination by Adsorptive Transfer Stripping Differential Pulse Voltammetry

“…Finally, a pH study was performed from 3.5 to 9.0 using 0.5 mM TET in a 50 mM phosphate buffer (see Figure S3). It is well known that the oxidation mechanism of TET is pH dependent (the lower pH the higher oxidation peak) [12]. However, only adsorbed TET molecules give a signal in AdTDPV.…”

“…On the other hand, tetracyclines are a kind of antibiotics with a chemical structure derived from a hydronaphthacene containing four fused rings (see Figure 1). All of them also contain a phenol group that is electroactive [12]. This group of antibiotics has been exhaustively employed in human and animal medicine and also as an additive in animal feed because of its broad spectrum antimicrobial activity and low cost [13].…”