Construction of a reference linkage map of Vitis amurensis and genetic mapping of Rpv8, a locus conferring resistance to grapevine downy mildew

Abstract

Downy mildew, caused by the oomycete Plasmopara viticola, is one of the major threats to grapevine. All traditional cultivars of grapevine (Vitis vinifera) are susceptible to downy mildew, the control of which requires regular application of fungicides. In contrast, many sources of resistance to P. viticola have been described in the Vitis wild species, among which is V. amurensis Rupr. (Vitaceae), a species originating from East Asia. A genetic linkage map of V. amurensis, based on 122 simple sequence repeat and 6 resistance gene analogue markers, was established using S1 progeny. This map covers 975 cM on 19 linkage groups, which represent 82% of the physical coverage of the V. vinifera reference genetic map. To measure the general level of resistance, the sporulation of P. viticola and the necrosis produced in response to infection, five quantitative and semi-quantitative parameters were scored 6 days post-inoculation on the S1 progeny. A quantitative trait locus (QTL) analysis allowed us to identify on linkage group 14 a major QTL controlling the resistance to downy mildew found in V. amurensis, which explained up to 86.3% of the total phenotypic variance. This QTL was named 'Resistance to Plasmopara viticola 8' (Rpv8).

Construction of a dense SNP map of a highly heterozygous diploid potato population and QTL analysis of tuber shape and eye depth

“…No QTL have been found on chromosomes 6, 8, 11, or 16, where we found QTL associated with sporulation and HR. While QTL on chromosome 14 have previously been found, the QTL found by Blasi et al (2011) and Venuti et al (2013) do not physically co-locate to the QTL we found on chromosome 14. The QTL on chromosome 7 found using the HC family single phenotype and Bayesian network analyses did not co-locate to one previously found on chromosome 7 by Moreira et al (2011).…”

“…(Buonassisi et al 2017). Most loci found have been qualitative (i.e., they explain the majority of the variation in the disease phenotype in a particular experiment), but a few explain only a small portion of the variation (Bellin et al 2009;Blasi et al 2011;Moreira et al 2011;Venuti et al 2013). Resistance dependent on a single dominant locus is not seen as being durable, especially to an air-borne outcrossing pathogen like the one that causes grapevine downy mildew (Buonassisi et al 2017;McDonald and Linde 2002).…”

Detection of downy and powdery mildew resistance QTL in a ‘Regent’ × ‘RedGlobe’ population

“…Additional to this major locus, two minor loci were identified on chromosomes 5 and 20, but were not assigned specific names. Several genetic maps have been constructed for grapevine (Dalbó et al 2000;Doligez et al 2002;Grando et al 2003;Adam-Blondon et al 2004;Fischer et al 2004;Riaz et al 2004;Doligez et al 2006;Lowe and Walker 2006;Di Gaspero et al 2007;Troggio et al 2007;Welter et al 2007;Vezzulli et al 2008;Bellin et al 2009;Marguerit et al 2009;Moreira et al 2011;Blasi et al 2011;Blanc et al 2012). The first maps were constructed using mainly AFLP and RAPD markers.…”

“…A minor resistance QTL explaining up to 12.7 % of the phenotypic variance observed, was identified on chromosome 7 (Rpv7) of 'Bianca' in a cross between 'Chardonnay' and 'Bianca' using various methods of phenotypic scoring (Bellin et al 2009). An S1-selfed progeny of a hermaphrodite accession V. amurensis 'Ruprecht' revealed a major resistance locus on chromosome 14 linked to marker Chr14V015 located between markers VVIP05 and VVIP22 (Blasi et al 2011). This locus, called Rpv8, explained up to 86 % of the observed phenotypic variance.…”

“…Rpv13 mapped close to the reported location for Rpv1. In addition to the above loci several unnamed minor loci have been reported on chromosomes 8, 12, 15 and 17 Blasi et al 2011;Moreira et al 2011). …”