Construction of a recombinant live oral vaccine from a non-toxigenic strain of Vibrio cholerae O1 serotype Inaba biotype El Tor and assessment of its reactogenicity and immunogenicity in the rabbit model

Thungapathra, M.; Sharma, Charu; Gupta, Naveen; Ghosh, Ram Krishna; Mukhopadhyay, Asish K.; Koley, Hemanta; Nair, G. Balakrish; Ghosh, Amit

doi:10.1016/s0165-2478(99)00076-0

Cited by 26 publications

(21 citation statements)

References 25 publications

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“…Only 13 strains were identified as non invasiveO1 Vibrio cholerae, 3 were Vibrio vulnificus and 10 were Vibrio species. Most of the patients were adult men with cirrhosis and history of seawater exposure [29]. A similar case associated with prior gastrectomy was reported with the hypothesis that all the cases yet reported were immune deficient and this was the first case reported in healthy patient and susceptibility to such infection may have been enhanced by a prior gastrectomy for duodenal ulcer [30].…”

Section: Discussionsupporting

confidence: 53%

Isolation of Vibrio cholerae in Homogenized Tissues of Liver, Gall Bladder and Bile in Rabbit Model

Abdullah

Aamenah³

et al. 2014

Interdiscip J Microinflammation

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Vibrio cholerae O139 is well known as the causative agent of cholera. It is noninvasive but many reports suggested it to cause bacteremia which brings in a little controversy with the previously reported old literature especially after reported histopathological invasion pattern of Vibrio cholerae O139 and Vibrio cholerae O1 ElTor. The rabbits were processes for ileac loops inoculation of Vibrio cholerae O139 followed by biochemical and molecular analysis of the presence of Vibrio cholerae O139 in liver, gall bladder and bile. We concluded the presence of Vibrio cholerae in liver, bile and gall bladder homogenized tissue. Retrograde spreading of bacteria to the liver via the common bile duct was excluded by complete closure of the intestinal lumen distant to the duct in the intestinal lumen. However, the bacteria might traffic, probably via macrophages, to distant area in the body including the liver. Another study in future is a need to identify the lesions of Vibrio cholerae invasions within liver and gall bladder by immunohistochemistry and using GFP visibility for Vibrio cholerae passage.

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Section: Discussionsupporting

confidence: 53%

Isolation of Vibrio cholerae in Homogenized Tissues of Liver, Gall Bladder and Bile in Rabbit Model

Abdullah

Aamenah³

et al. 2014

Interdiscip J Microinflammation

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“…The DNA probes for hlyU, hlyA, tcpA and toxR were a 0.769-kb EcoRI fragment, a 1.7-kb EcoRI fragment, a 0.471-kb EcoRI fragment and a 0.9-kb Xba I-Sal I fragment respectively, obtained from plasmids pHU2, pGT89, pTcpA and pToxRII, respectively [16]. All these gene-speci®c sequences were ampli®ed from V. cholerae 569B genomic DNA with various oligonucleotide primers (Table 1).…”

Section: Preparation Of Dna Probesmentioning

confidence: 99%

Comparison of antibiogram, virulence genes, ribotypes and DNA fingerprints of Vibrio cholerae of matching serogroups isolated from hospitalised diarrhoea cases and from the environment during 1997–1998 in Calcutta, India

Chakraborty¹,

Garg²,

Ramamurthy³

et al. 2001

Journal of Medical Microbiology

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This study identi®ed 17 matching serogroups of Vibrio cholerae belonging to serogroups other than O1 and O139 isolated from human cases and from the environment during a concurrent clinical and environmental study conducted in Calcutta, a cholera endemic area. Isolates within these matching serogroups were compared by various phenotypic and genotypic traits to determine if the environment was the source of the organisms associated with the disease. Clinical strains of V. cholerae were resistant to a greater number of drugs and exhibited multi-drug resistance compared with their environmental counterparts. Except for the presence of the genes for the El Tor haemolysin and the regulatory element ToxR in most of the strains of V. cholerae examined, non-O1, non-O139 V. cholerae strains lacked most of the other known virulence traits associated with toxigenic V. cholerae O1 or O139. Restriction fragment-length polymorphism of virulence-associated genes, ribotypes and DNA ®ngerprints of strains of matched serogroups showed considerable diversity, although some gene polymorphisms and ribotypes of a few strains of different serogroups were similar. It is concluded that despite sharing the same serogroup, environmental and clinical isolates were genetically heterogeneous and were of different lineages.

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“…15 Another live oral attenuated cholera vaccine known as VA1.4, created in India and manufactured by Shantha Biotechnics, Ltd., will shortly undergo phase III field trials in Kolkata. 16 India, which comprises 28 states and 7 union territories, has a total population of 1.15 billion people. Nearly twothirds of them live in rural areas, where only 28% of households use piped drinking water and 26% of households have access to good sanitation.…”

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confidence: 99%

Cholera in India: an analysis of reports, 1997–2006

Kanungo¹,

Sah²,

Lopez³

et al. 2010

Bull World Health Org

107

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Construction of a recombinant live oral vaccine from a non-toxigenic strain of Vibrio cholerae O1 serotype Inaba biotype El Tor and assessment of its reactogenicity and immunogenicity in the rabbit model

Cited by 26 publications

References 25 publications

Isolation of Vibrio cholerae in Homogenized Tissues of Liver, Gall Bladder and Bile in Rabbit Model

Isolation of Vibrio cholerae in Homogenized Tissues of Liver, Gall Bladder and Bile in Rabbit Model

Comparison of antibiogram, virulence genes, ribotypes and DNA fingerprints of Vibrio cholerae of matching serogroups isolated from hospitalised diarrhoea cases and from the environment during 1997–1998 in Calcutta, India

Cholera in India: an analysis of reports, 1997–2006

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