Construction of a Novel Ferroptosis-Related Gene Signature of Atherosclerosis

Huang, Tao; Wang, Kangjie; Li, Yuewei; Ye, Yanchen; Chen, Yangxin; Wang, Jingfeng; Yao, Chen

doi:10.3389/fcell.2021.800833

Cited by 18 publications

(17 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, TLR5-NOX4 signal cascades can stimulate proinflammatory cytokine expression through activation of NF- κ B or regulate smooth muscle cell migration through the TLR5-NOX4-RAC-JNK axis, which leads to the formation of neointimal plaques in atherosclerosis [ 33 ]. In addition, HMOX1 has been identified as a key ferroptosis-related gene in carotid atherosclerosis; however, it has not been thoroughly investigated yet [ 34 , 35 ]. In our study, we will further investigate genes associated with ferroptosis in carotid atherosclerosis.…”

Section: Discussionmentioning

confidence: 99%

Integrated Bioinformatics-Based Identification of Ferroptosis-Related Genes in Carotid Atherosclerosis

Wei

Wang

et al. 2022

Disease Markers

View full text Add to dashboard Cite

Background. Ferroptosis, a type of cell death caused by phospholipid peroxidation, has lately been linked to the onset and development of numerous illnesses. Numerous investigations have demonstrated the close relationship between lipid peroxidation and carotid atherosclerosis. In order to get new knowledge for targeted therapy, bioinformatics analysis was employed in this study to discover the probable ferroptosis-related genes of carotid atherosclerosis. Methods. The GSE43292 gene expression dataset was downloaded from the Gene Expression Omnibus (GEO) database. The differentially expressed ferroptosis-related genes were screened by R software and then analyzed by protein-protein interaction (PPI) network, differential gene correlation analysis, Kyoto Encyclopedia of Gene and Genome (KEGG) pathway, and Gene Ontology (GO) terminology enrichment analysis to explore the functional role. Result. In samples of atherosclerosis, we found 33 ferroptosis genes that were differentially expressed, including 21 upregulated genes and 12 downregulated genes. These differentially elevated genes were mainly connected to the ferroptosis and glutathione metabolism pathways, according to GO and KEGG enrichment analysis. We also discovered 10 hub genes and 2 important modules through the analysis of the PPI network and the creation of key modules. Conclusion. The current findings imply that the carotid atherosclerosis phenomenon involves ferroptosis, and 10 important genes associated to ferroptosis may play a role in the development of carotid atherosclerosis. This study offered a novel approach to future research on the carotid atherosclerosis pathogenic processes and treatment targets.

show abstract

Section: Discussionmentioning

confidence: 99%

Integrated Bioinformatics-Based Identification of Ferroptosis-Related Genes in Carotid Atherosclerosis

Wei

Wang

et al. 2022

Disease Markers

View full text Add to dashboard Cite

show abstract

“…Then, we screened for differentially expressed genes (DEGs) by the R package “limma”, which filters for |log2fold-change (FC)| > 1 and adjusted p < 0.05. Moreover, we analyzed this DEGs using the “clusterProfiler” R package for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways ( Huang et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

A novel signature of cuproptosis-related lncRNAs predicts prognosis in glioma: Evidence from bioinformatic analysis and experiments

Chen

Gao

et al. 2023

Front. Pharmacol.

View full text Add to dashboard Cite

Background: Glioma patients often experience unfavorable outcomes and elevated mortality rates. Our study established a prognostic signature utilizing cuproptosis-associated long non-coding RNAs (CRLs) and identified novel prognostic biomarkers and therapeutic targets for glioma.Methods: The expression profiles and related data of glioma patients were obtained from The Cancer Genome Atlas, an accessible online database. We then constructed a prognostic signature using CRLs and evaluated the prognosis of glioma patients by means of Kaplan-Meier survival curves and receiver operating characteristic curves. A nomogram based on clinical features was employed to predict the individual survival probability of glioma patients. Functional enrichment analysis was conducted to identify crucial CRL-related enriched biological pathways. The role of LEF1-AS1 in glioma was validated in two glioma cell lines (T98 and U251).Results: We developed and validated a prognostic model for glioma with 9 CRLs. Patients with low-risk had a considerably longer overall survival (OS). The prognostic CRL signature may serve independently as an indicator of prognosis for glioma patients. In addition, functional enrichment analysis revealed significant enrichment of multiple immunological pathways. Notable differences were observed between the two risk groups in terms of immune cell infiltration, function, and immune checkpoints. We further identified four drugs based on their different IC50 values from the two risk groups. Subsequently, we discovered two molecular subtypes of glioma (cluster one and cluster two), with the cluster one subtype exhibiting a remarkably longer OS compared to the cluster two subtype. Finally, we observed that inhibition of LEF1-AS1 curbed the proliferation, migration, and invasion of glioma cells.Conclusion: The CRL signatures were confirmed as a reliable prognostic and therapy response indicator for glioma patients. Inhibition of LEF1-AS1 effectively suppressed the growth, migration, and invasion of gliomas; therefore, LEF1-AS1 presents itself as a promising prognostic biomarker and potential therapeutic target for glioma.

show abstract

“…In recent years, increasing evidence has indicated that ferroptosis driven by iron-dependent lipid peroxidation plays a central role in cardiovascular diseases (CVDs), such as cardiomyopathy, myocardial ischemia/reperfusion injury (MI/RI), heart failure, and atherosclerosis. Inhibition of ferroptosis in cardiomyocytes has been implicated in alleviation of myocardial damage caused at least in part by ferroptosis [ 3 – 5 ] .…”

Section: Introductionmentioning

confidence: 99%

Multifaceted role of ferroptosis in cardiovascular disease

Li¹,

Zhu²,

Chen³

et al. 2023

ABBS

View full text Add to dashboard Cite

Ferroptosis is a newly identified form of non-apoptotic cell death characterised primarily by iron-dependent lipid peroxidation. It differs morphologically, biochemically, and genetically from other forms of cell death, such as apoptosis, autophagy, and necrosis. Although the molecular mechanism underlying ferroptosis remains unclear, multiple biological processes, such as iron metabolism, lipid peroxides, and systems, such as the glutathione system and the tetrahydrobiopterin/coenzyme Q10 system, appear to be involved. While the contribution of ferroptotic mechanisms to human diseases is not clear, recent studies have identified a number of ferroptosis-related genes. Cardiovascular diseases are the main cause of death globally. In this review, we outline the progress regarding the emerging role of ferroptosis in the pathogenesis of cardiac pathophysiological conditions and the association of ferroptosis with cardiomyopathy, myocardial ischemia-reperfusion injury, heart failure, and atherosclerosis. We further summarise newly discovered ferroptotic targets for the development of therapies for cardiovascular diseases. Finally, we discuss the current challenges and future research directions in cardiovascular disease treatments.

show abstract

Construction of a Novel Ferroptosis-Related Gene Signature of Atherosclerosis

Cited by 18 publications

References 43 publications

Integrated Bioinformatics-Based Identification of Ferroptosis-Related Genes in Carotid Atherosclerosis

Integrated Bioinformatics-Based Identification of Ferroptosis-Related Genes in Carotid Atherosclerosis

A novel signature of cuproptosis-related lncRNAs predicts prognosis in glioma: Evidence from bioinformatic analysis and experiments

Multifaceted role of ferroptosis in cardiovascular disease

Contact Info

Product

Resources

About