Construction of 2 intraspecific linkage maps and identification of resistance QTLs forPhytophthora capsiciroot-rot and foliar-blight diseases of pepper (CapsicumannuumL.)

Ogundiwin, Ebenezer A.; Berke, Terry F; Massoudi, Mark; Black, L. M.; Huestis, Gordon M.; Choi, Doil; Lee, Sanghyeob; Prince, James P.

doi:10.1139/g05-028

Cited by 84 publications

(73 citation statements)

References 34 publications

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“…S19 was mapped to P9, $6 cM below TG263 and centromeric with respect to the position for the 1-kb band of A2 (Figure 6), demonstrating that Bs2 resides on P9 in a region of the pepper genome that is orthologous to the top arm of potato XII that includes the fractionated orthologs Rx and Gpa2. This is consistent with results from others who have located several PCR markers with similarity to Bs2 on this chromosome (Ogundiwin et al 2005;Sugita et al 2006;DjianCaporalino et al 2007).…”

Section: Resultssupporting

confidence: 93%

The Fractionated Orthology of Bs2 and Rx/Gpa2 Supports Shared Synteny of Disease Resistance in the Solanaceae

et al. 2009

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Comparative genomics provides a powerful tool for the identification of genes that encode traits shared between crop plants and model organisms. Pathogen resistance conferred by plant R genes of the nucleotide-binding-leucine-rich-repeat (NB-LRR) class is one such trait with great agricultural importance that occupies a critical position in understanding fundamental processes of pathogen detection and coevolution. The proposed rapid rearrangement of R genes in genome evolution would make comparative approaches tenuous. Here, we test the hypothesis that orthology is predictive of R-gene genomic location in the Solanaceae using the pepper R gene Bs2. Homologs of Bs2 were compared in terms of sequence and gene and protein architecture. Comparative mapping demonstrated that Bs2 shared macrosynteny with R genes that best fit criteria determined to be its orthologs. Analysis of the genomic sequence encompassing solanaceous R genes revealed the magnitude of transposon insertions and local duplications that resulted in the expansion of the Bs2 intron to 27 kb and the frequently detected duplications of the 59-end of R genes. However, these duplications did not impact protein expression or function in transient assays. Taken together, our results support a conservation of synteny for NB-LRR genes and further show that their distribution in the genome has been consistent with global rearrangements.

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Section: Resultssupporting

confidence: 93%

The Fractionated Orthology of Bs2 and Rx/Gpa2 Supports Shared Synteny of Disease Resistance in the Solanaceae

et al. 2009

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“…Linkage analysis revealed that many molecular markers were located at the same loci: 224 markers with clear and reproducible banding patterns were selected as framework markers. Moreover, 83 pairs of PCR primers described by Lee et al (2004) and Ogundiwin et al (2005) and 62 pairs of microsatellite primers obtained from the database, were used in association with already published linkage maps. The map, with a total of 16 linkage groups (LGs) and covering a total distance of 1100.5 cM was used for QTL analysis.…”

Section: Methodsmentioning

confidence: 99%

“…The nearest AFLP marker, M9E3-11, was a dominant marker present in 'AC2258' (Table 3). Ogundiwin et al (2005) detected 16 QTLs from Phyto.A to P on the RILs derived from the same resistant parent as that we used. Phyt-1 detected on LG7 (pepper chromosome 5) appeared to correspond to Phyto.P detected by Ogundiwin et al (2005), because it was located on the same chromosome.…”

Section: Qtl Analysismentioning

confidence: 99%

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QTL Analysis for Resistance to Phytophthora Blight (Phytophthora capsici Leon.) Using an Intraspecific Doubled-Haploid Population of Capsicum annuum

et al. 2006

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A doubled-haploid (DH) population (n = 176) obtained by anther culture of an F 1 hybrid between a line susceptible to Phytophthora capsici 'K9-11' (Capsicum annuum L.) and a line resistant to P. capsici 'AC2258' (C. annuum L.) was inoculated with P. capsici. QTL analysis of the resistance was performed using a linkage map consisting of 16 linkage groups (LGs), covering a total distance of 1100.5 cM. Three QTLs were detected on LG1, LG6 and LG7. The QTL with the highest LOD score, detected on LG7, explained 82.7% of the phenotypic variance with a LOD score of 67.02. This QTL was designated as Phyt-1. The nearest marker was an AFLP marker, M10E3-6. The second QTL, designated as Phyt-2, was found on LG1. It explained 6.4% of the phenotypic variance with a LOD score of 2.54. The nearest RAPD marker was RP13-1. The other QTL, designated as Phyt-3, which was found on LG6, explained 5.6% of the phenotypic variance with a LOD score of 2.20. The nearest AFLP marker was M9E3-11. It was confirmed that the lines with a high resistance could be efficiently selected by using two markers, M10E3-6 and RP13-1, simultaneously. The presence of both Phyt-1 and Phyt-2 under homozygous conditions may enable to breed resistant cultivars of sweet pepper. The molecular markers identified in the present study could be useful for marker-assisted selection (MAS) in order to breed sweet pepper cultivars with a high resistance to P. capsici using 'AC2258' as a source of resistance genes.

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“…Despite these genetic material did not amplify the SCAR marker OpD04.717, can be used to search for other markers associated with genes that confer resistance to the Oomycete like: CaRGA2, CABPR1, CABGLU, CAPO1, CASC1 (Silvar et al, 2008;Zhang et al, 2013), or QTLs Phyto.4.1, Phyto.5.1, Phyto.6.1, Phyto.11.1 and Phyto12.1, located on chromosomes 4, 5, 11 and 12 respectively (Thabuis et al 2003). Because the resistance to this pathogen is polygenic (Pochard and Daubèze, 1980;Ogundiwin et al, 2005;Minamiyama et al, 2007), it is difficult to explain the resistance to the disease just through marker OpD04.717.…”

Section: Resultados Y Discusiónmentioning

confidence: 99%

Identificación de fuentes de resistencia a pudriciones de la raíz en germoplasma de chile serrano (Capsicum annuum L.)

Aguilar

Guerra²,

Meraz

et al. 2017

Remexca

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ResumenEn México las pérdidas en la producción de chile (Capsicum spp.) ocasionadas por infección con Phytophthora capsici Leo; han llegado a ser de hasta 100% en áreas específicas del Bajío y Puebla, por lo que se ha planteado enfrentar el problema fitosanitario mediante la búsqueda de resistencia genética en accesiones de chile serrano obtenidas a partir de colectas en diferentes regiones del país. En el presente trabajo de investigación se utilizó el marcador SCAR OpD04.717 para identificar fuentes de resistencia a dicho patógeno a nivel molecular, se consideraron las accesiones con resistencia a P. capsici cuando amplificaron una banda de 717 pb. Así también se realizaron pruebas de reacción a P. capsici bajo condiciones in vitro, con la cepa PCT-17 previamente aislada, y se obtuvo el porcentaje de supervivencia de 142 accesiones de chile más los dos testigos (SCM334, resistente y MIRASOL, susceptible al hongo). La accesión que mostró mayor resistencia a la cepa fue BGS41 en comparación con SCM334. Únicamente se identificaron accesiones de chile serrano con resistencia a P. capsici mediante pruebas de reacción in vitro. La no identificación de fuentes de resistencia al hongo a nivel AbstractIn Mexico production losses in pepper (Capsicum spp.) caused by infection of Phytophthora capsici Leo, has reached up to 100% in specific areas from the Bajio and Puebla, so it has been lay out to face the phytosanitary problem through the search of genetic resistance in serrano pepper accessions obtained from collections in different regions. In the present study the SCAR marker OpD04.717 was used to identify sources of resistance to the pathogen at molecular level, the accessions were considered resistant to P. capsici when amplified at band of 717 bp. Also reaction tests to P. capsici under in vitro conditions with strain PCT-17 previously isolated were carried out and the survival rate from 142 pepper accessions plus two checks (SCM334, resistant and MIRASOL susceptible to fungus) was obtained. The accession that showed greater resistance to strain was BGS41 compared to SCM334. Serrano pepper accessions with resistance to P. capsici were identified by in vitro test reactions. Not being able to identify sources of resistance to the fungus at molecular level in serrano pepper accessions could be due to the presence of an allele marker associated with resistance locus in a given accession / population does 1508 Rev. Mex. Cienc. Agríc. Vol.6 Núm. 7 28 de septiembre -11 de noviembre, 2015Reinaldo Méndez Aguilar et al. molecular en accesiones de chile serrano se pudo deber a que la presencia de un alelo marcador asociado con un locus de resistencia en una accesión/población determinada no implica necesariamente la misma asociación en diferentes accesiones. Se identificaron accesiones de chile serrano con utilidad potencial como nuevas fuentes de resistencia a pudriciones de la raíz (P. capsici) para programas de mejoramiento genético tradicional pero no para selección asistida por marcadores (MAS) mediante OpD04.717.Palabra...

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Construction of 2 intraspecific linkage maps and identification of resistance QTLs forPhytophthora capsiciroot-rot and foliar-blight diseases of pepper (CapsicumannuumL.)

Cited by 84 publications

References 34 publications

The Fractionated Orthology of Bs2 and Rx/Gpa2 Supports Shared Synteny of Disease Resistance in the Solanaceae

The Fractionated Orthology of Bs2 and Rx/Gpa2 Supports Shared Synteny of Disease Resistance in the Solanaceae

QTL Analysis for Resistance to Phytophthora Blight (Phytophthora capsici Leon.) Using an Intraspecific Doubled-Haploid Population of Capsicum annuum

Identificación de fuentes de resistencia a pudriciones de la raíz en germoplasma de chile serrano (Capsicum annuum L.)

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