Construction, electrochemically biosensing and discrimination of recombinant plasmid (pEThIL-2) on the basis of interleukine-2 DNA insert

Hejazi, Mohammad Saeid; Pournaghi-Azar, M.H.; Alipour, Esmaeel; Karimi, Farrokh

doi:10.1016/j.bios.2008.01.021

Cited by 47 publications

(25 citation statements)

References 30 publications

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“…Research has demonstrated the utility of graphite electrodes [23] in the construction of electrochemical biosensor utilizing oligonucleotides [12,15]. The good sensitivity of the carbon electrodes in detecting the oxidation of nucleic acids makes them widely useful in DNA research, especially when inexpensive pencil graphite electrode is used [24].…”

Section: Resultsmentioning

confidence: 99%

“…Due to their high sensitivity, small dimensions, low cost, and compatibility with microfabrication technology electrochemical transducers are often used for detecting hybridization events [12,15,16]. Sensitive electrochemical signaling strategies are based on the direct or catalyzed oxidation of nucleic acid nucleotides, as well as the redox reactions of reporter molecules in the indirect detection using chemical indicators [17,18].…”

Section: Introductionmentioning

confidence: 99%

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Label-Free Electrochemical Detection of the Specific Oligonucleotide Sequence of Dengue Virus Type 1 on Pencil Graphite Electrodes

Souza

Nascimento

Santana

et al. 2011

Sensors

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A biosensor that relies on the adsorption immobilization of the 18-mer single-stranded nucleic acid related to dengue virus gene 1 on activated pencil graphite was developed. Hybridization between the probe and its complementary oligonucleotides (the target) was investigated by monitoring guanine oxidation by differential pulse voltammetry (DPV). The pencil graphite electrode was made of ordinary pencil lead (type 4B). The polished surface of the working electrode was activated by applying a potential of 1.8 V for 5 min. Afterward, the dengue oligonucleotides probe was immobilized on the activated electrode by applying 0.5 V to the electrode in 0.5 M acetate buffer (pH 5.0) for 5 min. The hybridization process was carried out by incubating at the annealing temperature of the oligonucleotides. A time of five minutes and concentration of 1 μM were found to be the optimal conditions for probe immobilization. The electrochemical detection of annealing between the DNA probe (TS-1P) immobilized on the modified electrode, and the target (TS-1T) was achieved. The target could be quantified in a range from 1 to 40 nM with good linearity and a detection limit of 0.92 nM. The specificity of the electrochemical biosensor was tested using non-complementary sequences of dengue virus 2 and 3.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Label-Free Electrochemical Detection of the Specific Oligonucleotide Sequence of Dengue Virus Type 1 on Pencil Graphite Electrodes

Souza

Nascimento

Santana

et al. 2011

Sensors

View full text Add to dashboard Cite

show abstract

“…Because of some limitations of analytical practice, toxicity and insensitivity, development of inexpensive and sensitive indicator for the study of interaction between DNA and nucleic acid binding molecules is important. The utility of any indicator is intimately dependent on its viability for producing diagnoses with high confidence [26,27].…”

Section: Introductionmentioning

confidence: 99%

Celestine blue as a new indicator in electrochemical DNA biosensors

et al. 2015

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Celestine blue (CB) was introduced as a new electroactive indicator in DNA biosensors. The interaction of CB with DNA was investigated by electrochemical and spectroscopic methods. The effect of buffer kind and pH on the electrochemical behavior of CB was studied. The peak currents of CB were linearly related to DNA concentration in the range of 5.0×10 −9 to 1.0×10 −7 mol/L. The detection limit of this approach was 4.76×10 −10 mol/L. Based on spectrometry data a hypochromic effect was observed in UV-Vis spectra of CB with increasing DNA concentration. The results illustrate the possible interaction mode between CB and DNA is electrostatic binding. celestine blue, differential pulse voltammetry, DNA biosensor, electroactive indicator

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“…We did not find any report concerning the development of electrochemical biosensor for direct detection and discrimination of DNA sequence and single base mismatch in either double-stranded oligonucleotide (ds-oligonucleotide) chains or double-strand PCR products without denaturating dsDNA into ssDNA. We have previously reported the electrochemical detection of single-stranded DNA (ssDNA), using methylene blue (MB) [19][20][21] and brilliant cresyl blue (BCB) [22] reduction signals and also utilizing guanine oxidation signal [23][24][25]. Recently we have reported the development of a electrochemical biosensor for direct detection of double-stranded oligonucleotide corresponding to hepatitis C virus genotype 3a [26].…”

Section: Introductionmentioning

confidence: 99%

Development of a Novel Electrochemical Biosensor for Detection and Discrimination of DNA Sequence and Single Base Mutation in dsDNA Samples Based on PNA‐dsDNA Hybridization – a new Platform Technology

et al. 2010

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In spite of the extensive attention paid on the development of various DNA detection strategies, very few studies have been reported regarding direct detection of DNA sequence and mutation in dsDNA. Here, we describe the feasibility of detection and discrimination of target DNA sequences and single base mutations (SBM) directly in double-stranded oligonucleotides and PCR products without the need for denaturation of the target dsDNA samples. This goal was achieved by employing a peptide nucleic acid (PNA) chain, self-assembled on the gold electrode as a probe, which binds to dsDNA and forms PNA-dsDNA hybrid.

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Construction, electrochemically biosensing and discrimination of recombinant plasmid (pEThIL-2) on the basis of interleukine-2 DNA insert

Cited by 47 publications

References 30 publications

Label-Free Electrochemical Detection of the Specific Oligonucleotide Sequence of Dengue Virus Type 1 on Pencil Graphite Electrodes

Label-Free Electrochemical Detection of the Specific Oligonucleotide Sequence of Dengue Virus Type 1 on Pencil Graphite Electrodes

Celestine blue as a new indicator in electrochemical DNA biosensors

Development of a Novel Electrochemical Biosensor for Detection and Discrimination of DNA Sequence and Single Base Mutation in dsDNA Samples Based on PNA‐dsDNA Hybridization – a new Platform Technology

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