Construction and<i>in vitro</i>testing of a cellulose dura mater graft

Goldschmidt, Ezequiel; Cacicedo, Maximiliano L.; Kornfeld, Sebastián; Valinoti, Marina; Ielpi, Marcelo; Ajler, Pablo; Yampolsky, Claudio; Rasmussen, Jorge; Castro, Guillermo R.; Argibay, Pablo

doi:10.1080/01616412.2015.1122263

Cited by 18 publications

(10 citation statements)

References 23 publications

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“…For reconstruction of the dura mater , bacterial cellulose membranes were tested as potential dural patches to prevent leakage of cerebrospinal fluid, which is a common complication after cranial and spinal surgery. These membranes supported the attachment and the viability of human dural fibroblasts [213].…”

Section: Recent Use Of Nanocellulose In Tissue Engineering and Tismentioning

confidence: 99%

Versatile Application of Nanocellulose: From Industry to Skin Tissue Engineering and Wound Healing

et al. 2019

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Nanocellulose is cellulose in the form of nanostructures, i.e., features not exceeding 100 nm at least in one dimension. These nanostructures include nanofibrils, found in bacterial cellulose; nanofibers, present particularly in electrospun matrices; and nanowhiskers, nanocrystals, nanorods, and nanoballs. These structures can be further assembled into bigger two-dimensional (2D) and three-dimensional (3D) nano-, micro-, and macro-structures, such as nanoplatelets, membranes, films, microparticles, and porous macroscopic matrices. There are four main sources of nanocellulose: bacteria (Gluconacetobacter), plants (trees, shrubs, herbs), algae (Cladophora), and animals (Tunicata). Nanocellulose has emerged for a wide range of industrial, technology, and biomedical applications, namely for adsorption, ultrafiltration, packaging, conservation of historical artifacts, thermal insulation and fire retardation, energy extraction and storage, acoustics, sensorics, controlled drug delivery, and particularly for tissue engineering. Nanocellulose is promising for use in scaffolds for engineering of blood vessels, neural tissue, bone, cartilage, liver, adipose tissue, urethra and dura mater, for repairing connective tissue and congenital heart defects, and for constructing contact lenses and protective barriers. This review is focused on applications of nanocellulose in skin tissue engineering and wound healing as a scaffold for cell growth, for delivering cells into wounds, and as a material for advanced wound dressings coupled with drug delivery, transparency and sensorics. Potential cytotoxicity and immunogenicity of nanocellulose are also discussed.

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Section: Recent Use Of Nanocellulose In Tissue Engineering and Tismentioning

confidence: 99%

Versatile Application of Nanocellulose: From Industry to Skin Tissue Engineering and Wound Healing

et al. 2019

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show abstract

“…For reconstruction of the dura mater, bacterial cellulose membranes were tested as potential dural patches to prevent leakage of cerebrospinal fluid, which is a common complication after cranial and spinal surgery. These membranes supported the attachment and the viability of human dural fibroblasts [186].…”

Section: Recent Use Of Nanocellulose In Tissue Engineering and Tissuementioning

confidence: 88%

Nanocellulose in Biotechnology and Medicine: Focus on Skin Tissue Engineering and Wound Healing

Bacakova¹,

Pajorová²,

Bačáková³

et al. 2018

Preprint

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Nanocellulose is cellulose in the form of nanostructures, i.e. features not exceeding 100 nm at least in one dimension. These nanostructures include nanofibrils, e.g. in bacterial cellulose; nanofibers, e.g. in electrospun matrices; nanowhiskers and nanocrystals. These structures can be further assembled into bigger 2D and 3D nano-, micro- and macro-structures, such as nanoplatelets, membranes, films, microparticles and porous macroscopic matrices. There are four main sources of nanocellulose: bacteria (Gluonacetobacter), plants (trees, shrubs, herbs), algae (Cladophora) and animals (Tunicata). Nanocellulose has emerged for a wide range of industrial, technology and biomedical applications, e.g. for adsorption, ultrafiltration, packaging, conservation of historical artifacts, thermal insulation and fire retardation, energy extraction and storage, acoustics, sensorics, controlled drug delivery, and particularly for tissue engineering. Nanocellulose is promising for use in scaffolds for engineering of blood vessels, neural tissue, bone, cartilage, liver, adipose tissue, urethra and dura mater, for repairing connective tissue and congenital heart defects, and for constructing contact lenses and protective barriers. This review is focused on applications of nanocellulose in skin tissue engineering and wound healing as a scaffold for cell growth, for delivering cells into wounds, and as a material for advanced wound dressings coupled with drug delivery, transparency and sensorics. Potential cytotoxicity and immunogenicity of nanocellulose are also discussed.

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“…It has been shown that ROC in vitro could induce cell necrosis in a variety of tissues . ROC applied to human dural fibroblasts altered their cellular structure and reduced the cell viability . However, in‐vitro studies may overestimate these effects due to the absence of buffer elements present in a living organism.…”

Section: Discussionmentioning

confidence: 99%

“…21,22 ROC applied to human dural fibroblasts altered their cellular structure and reduced the cell viability. 20,36 However, in-vitro studies may overestimate these effects due to the absence of buffer elements present in a living organism. The in-vivo experiments in the current study were performed while the middle turbinate had normal blood flow preserving the buffer mechanisms normally present in the nasal mucosa, thus controlling for these factors.…”

Section: Discussionmentioning

confidence: 99%

Effect of oxidized cellulose on human respiratory mucosa and submucosa and its implications for endoscopic skull‐base approaches

Goldschmidt

Schneck

Gau

et al. 2019

Int Forum Allergy Rhinol

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Background Regenerated oxidized cellulose (ROC) sheets have gained popularity as an adjunct to a vascularized nasoseptal flap for closure of dural defects after endoscopic endonasal skull‐base approaches (EESBS). However, evidence supporting its impact on the healing process is uncertain. This study was performed to evaluate the impact of ROC on the nasal mucosa and assess its effects on tissue pH, structure, and cell viability. Methods In 5 patients, a 1‐cm2 piece of ROC gauze was placed on the surface of the middle turbinate before it was resected as part of a standard EESBS. Mucosa treated with ROC was separated from untreated mucosa and a histologic examination of structural changes in the respiratory epithelium was performed. To assess the effect of ROC on pH, increasing amounts of ROC were added to culture medium. Nasal fibroblasts viability was assessed in the presence of ROC before and after the pH was neutralized. Results Compared with unexposed controls, treated mucosa exhibited a higher incidence of cell necrosis and epithelial cell detachment. When added to Dulbecco's modified Eagle medium, ROC caused a dose‐dependent decrease in pH of the medium. Only 1 ± 0.8% of cultured fibroblasts exposed to the ROC‐induced acidic medium were alive, whereas 98.25 ± 0.5% of the cells were viable when the pH was neutralized (p < 0.001). Conclusion ROC applied in vivo to nasal mucosa induced epithelial necrosis likely by diminishing the medium pH, because pH neutralization prevents its effect. The ultimate effect of this material on the healing process is yet to be determined.

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Construction andin vitrotesting of a cellulose dura mater graft

Abstract: BC membranes are used in clinical practice to improve skin healing. In the presence of water, they form an elastic, nontoxic, and resistant biogel that can accommodate collagen and growth factors within their structure, thus BC is a good candidate for dural graft construction.

Cited by 18 publications

References 23 publications

Versatile Application of Nanocellulose: From Industry to Skin Tissue Engineering and Wound Healing

Versatile Application of Nanocellulose: From Industry to Skin Tissue Engineering and Wound Healing

Nanocellulose in Biotechnology and Medicine: Focus on Skin Tissue Engineering and Wound Healing

Effect of oxidized cellulose on human respiratory mucosa and submucosa and its implications for endoscopic skull‐base approaches

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