Construction and clinical validation of a sensitive and specific assay for serum mature calcitonin using monoclonal anti-peptide antibodies

Motté, Philippe; Vauzelle, Pascal; Gardet, P; Ghillani, Pascale; Caillou, Bernard; Parmentier, C; Bohuon, C; Bellet, Dominique

doi:10.1016/0009-8981(88)90365-8

Cited by 74 publications

(34 citation statements)

References 29 publications

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“…This is an IRMA method using two monoclonal antibodies, which are highly specific for calcitonin with no crossreaction with procalcitonin (26). The standard curve (0-500 pg/ml) is linear from 10 to 500 pg/ml and dilutions were performed for values above 400 pg/ml.…”

Section: Methodsmentioning

confidence: 99%

Progression of medullary thyroid carcinoma: assessment with calcitonin and carcinoembryonic antigen doubling times

Giraudet

Ghulzan²,

Aupérin³

et al. 2008

European Journal of Endocrinology

239

129

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Objective: The progression of medullary thyroid cancer is difficult to assess with imaging modalities; we studied the interest of calcitonin and carcinoembryonic antigen (CEA) doubling times and of Ki-67 labeling and mitotic index (MI). Patients and methods: Fifty-five consecutive medullary thyroid carcinoma (MTC) patients with elevated calcitonin levels underwent repeated imaging studies in order to assess tumor burden and progression status. We looked for relationships between tumor burden and levels of calcitonin and CEA and between progression status according to the response evaluation criteria in solid tumors (RECIST) and calcitonin and CEA doubling times, and Ki-67 labeling and MI. Results: The calcitonin and CEA levels were correlated with tumor burden. Ten patients with calcitonin levels below 816 pg/ml had no imaged tumor foci. Among the 45 patients with imaged tumor foci, 19 had stable disease and 26 had progressive disease, according to the RECIST. The calcitonin and CEA doubling times were strongly related to disease progression, with very few overlaps: 94% of patients with doubling times shorter than 25 months had progressive disease and 86% of patients with doubling times longer than 24 months had stable disease. Ki-67 labeling and MI were not significantly associated with disease progression. Conclusion: For MTC patients, the doubling times of both calcitonin and CEA are efficient tools for assessing tumor progression.

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Section: Methodsmentioning

confidence: 99%

Progression of medullary thyroid carcinoma: assessment with calcitonin and carcinoembryonic antigen doubling times

Giraudet

Ghulzan²,

Aupérin³

et al. 2008

European Journal of Endocrinology

239

129

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“…CT levels were determined using an IRMA (IRMA-CIS-Biointernational, Gif-Sur-Yvette, France), except in two patients who had undergone surgery before 1988 (9,10). Basal or CT/Pg levels above 10 pg/ml were considered abnormal in the postoperative period in all the patients.…”

Section: Study Populationmentioning

confidence: 99%

The clinical spectrum of RET proto-oncogene mutations in codon 790

Bihan¹,

Murat²,

Fysekidis³

et al. 2013

European Journal of Endocrinology

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Objective: Due to a strong genotype-phenotype correlation, the timing of prophylactic thyroidectomy in rearranged during transfection (RET) gene mutation carriers is usually dictated by genetic analysis. Subjects and methods: We report a nationwide retrospective study of the clinical data of 77 French patients from 19 families with a mutation in codon 790 of the RET proto-oncogene. Results: The average age at diagnosis was 35.6 yearsG20.5. Thirty-nine patients were women. Fiftyfive patients underwent operations for the treatment of medullary thyroid carcinoma (MTC) at the mean age of 38 years (4-82 years). The mean follow-up duration was 89 months. TNM staging was as follows: T0NxMx in 19, TxNxMx in 1, T1NxMx in 22, T1N1Mx in 8, T2N1Mx in 1 and T3N1Mx in four patients. In the T1/x-Nx group, 96% were considered cured after surgery. In the N1 group (nZ13), six patients had multifocal disease and five patients were cured. Age and gender were not significant predictors of remission. Twenty-two patients did not undergo an operation (age 1.5-78 years); among them, 11 patients had a mean basal calcitonin (CT) level of 9.8 pg/ml (2-24) after 53 months of follow-up. One patient had been operated on for phaeochromocytoma (PHEO), and their CT level remained normal for 262 months. Conclusions: This study confirms that RET 790 mutation is associated with a non-aggressive form of multiple endocrine neoplasia type 2, as 28% of the patients were followed up without thyroidectomy, 25% had been thyroidectomised with no tumour being detected and even patients with MTC had slowevolving disease. Moreover, only one patient had PHEO, and no-one had primary hyperparathyroidism.

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“…Although calcitonin concentrations are higher in men than in women and tend to decline with age, many laboratories use a cutoff value of 10 ng/L instead of a population-based reference interval. Prior studies have shown that basal calcitonin concentrations are below this threshold in a normal population (1,2 ) and in 90% of patients suffering from other nodular thyroid diseases (3 ). In clinical practice, a patient with a calcitonin concentration higher than 10 ng/L should undergo a pentagastrin test to exclude MCT.…”

Section: To the Editormentioning

confidence: 99%

“…The recent report by Gambino (1 ) brought attention to the often overlooked fact that fluoride does not prevent loss of plasma glucose during the first 30 -90 min (or longer) after blood collection (2 ). Although fluoride is effective in preventing later loss of glucose (1,2 ), the mechanism of the delay in its action is a matter of some interest.…”

Section: To the Editormentioning

confidence: 99%

See 1 more Smart Citation

Analytical Quality of Calcitonin Determination and Its Effect on the Adequacy of Screening for Medullary Carcinoma of the Thyroid

Cavalier¹,

Carlisi²,

Chapelle³

et al. 2008

Clinical Chemistry

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Construction and clinical validation of a sensitive and specific assay for serum mature calcitonin using monoclonal anti-peptide antibodies

Cited by 74 publications

References 29 publications

Progression of medullary thyroid carcinoma: assessment with calcitonin and carcinoembryonic antigen doubling times

Progression of medullary thyroid carcinoma: assessment with calcitonin and carcinoembryonic antigen doubling times

The clinical spectrum of RET proto-oncogene mutations in codon 790

Analytical Quality of Calcitonin Determination and Its Effect on the Adequacy of Screening for Medullary Carcinoma of the Thyroid

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