Analytical Quality of Calcitonin Determination and Its Effect on the Adequacy of Screening for Medullary Carcinoma of the Thyroid

Cavalier, Étienne; Carlisi, Agnès; Chapelle, Jean-Paul; Delanaye, Pierre

doi:10.1373/clinchem.2007.100636

Cited by 21 publications

(10 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some anticoagulants are often combined with a preservative such as NaF to inhibit glycolysis of blood glucose by exerting its effects on enolase. Furthermore, NaF may inhibit the activity of many other enzymes such as urease, cholinesterase, and also bacterial proliferation, thus reducing losses of nitroaromatic compounds 60,61 . Addition of NaF to various biological samples (e.g.…”

Section: Resultsmentioning

confidence: 99%

Stability of synthetic cathinones in clinical and forensic toxicological analysis—Where are we now?

Aldubayyan

Castrignanò

Elliott

et al. 2020

Drug Testing and Analysis

View full text Add to dashboard Cite

Understanding the stability of analyzed drugs in biological samples is a crucial part for an appropriate interpretation of the analytical findings. Synthetic cathinones, as psychoactive stimulants, belong to a major class of new psychoactive substances. As they are subject to several degradation pathways, they are known to clinical and forensic toxicologists as unstable analytes in biological samples. When interpreting analytical data of synthetic cathinones in biological samples, analysts must be aware that the concentration of analytes may not accurately reflect the levels at the time they were acquired owing to many factors. This review provides (i) an overview of the current scientific knowledge on the stability of synthetic cathinones and/or metabolites in various human biological samples with a focus on factors that may deteriorate their stability-such as storage temperature, length of storage, matrix, pH, type of preservatives, concentration of analytes, and the chemistry of the analytesand (ii) possible solutions on how to avoid such degradation. The PubMed database as well as Google Scholar was thoroughly searched to find published studies on the stability of synthetic cathinones since 2007 by searching specific keywords. A total of 23 articles met the inclusion criteria and were included in this review. Synthetic cathinones that carry methylenedioxy or N-pyrrolidine ring showed higher degradation resistance over other substituted groups. Acidification of samples pH plays a crucial role at increasing the stability of cathinones even with analytes that were frequently considered as poorly stable. This review also provides several recommendations for best practice in planning the experimental design, preservation, and storage conditions in order to minimize synthetic cathinones' degradation in human biological samples.

show abstract

Section: Resultsmentioning

confidence: 99%

Stability of synthetic cathinones in clinical and forensic toxicological analysis—Where are we now?

Aldubayyan

Castrignanò

Elliott

et al. 2020

Drug Testing and Analysis

View full text Add to dashboard Cite

show abstract

“…Later in 2006, Diasorin (DS) also launched an automated CT assay. However, as medical laboratories have moved from radioisotopic to fully automated methods, such a change may pose problems, because some chemiluminescent methods lack adequate sensitivity and specificity (3,25).…”

Section: Discussionmentioning

confidence: 99%

Calcitonin screening in patients with thyroid nodules

Schneider¹,

Kobe²,

Schmidt³

et al. 2012

Nuklearmedizin

View full text Add to dashboard Cite

show abstract

“…However, CT assays suffer pre-analytic and analytic drawbacks: i. CT is prone to relatively rapid in vitro degradation by serum proteases which makes rapid processing of samples mandatory [3]; ii. the presence of various different immunoreactive isoforms and fragments, which can lead to inaccurate results (usually false low) as well as poor comparability of results obtained by different assays [4][5][6][7]. Carcinoembryonic antigen (CEA), a nonspecific marker, is also used for monitoring MTC patients, being a reliable predictor of disease progression [8]; however, it is an insensitive marker for early postoperative detection of MTC persistence/relapse [9].…”

Section: Introductionmentioning

confidence: 99%

Clinical performance of calcitonin and procalcitonin Elecsys^® immunoassays in patients with medullary thyroid carcinoma

Giovanella

Fontana

Keller

et al. 2020

Clinical Chemistry and Laboratory Medicine (CCLM)

View full text Add to dashboard Cite

ObjectivesMedullary thyroid carcinoma (MTC) is caused by a malignant transformation in the parafollicular C-cells of the thyroid, where calcitonin (CT) is released. Nowadays, CT is the main tumor marker used in the diagnosis and follow-up of MTC patients. Nonetheless, procalcitonin (PCT) has recently been proposed as a useful complementary/alternative biomarker in MTC. Our aims were to investigate the diagnostic performance of CT and PCT and their combination in the differential diagnosis between active and inactive MTC and between MTC and non-MTC thyroid diseases, respectively.MethodsSerum samples were collected from 16 patients with active (i.e. primary tumour before surgery or post-surgical recurrent disease) and 23 with inactive (i.e. complete remission) MTC, 125 patients with non-MTC benign thyroid disease and 62 patients with non-MTC thyroid cancers, respectively. Elecsys® CT and PCT measurements were simultaneously performed on the Cobas e601 platform (Roche Diagnostics, Rotkreutz, Switzerland).ResultsBoth CT and PCT median values in active MTC (94 pmol/L and 1.17 ng/mL, respectively) were significantly higher compared with inactive MTC (0.28 and 0.06) and either benign (0.37 and 0.06) or malignant (0.28 and 0.06) non-MTC. Undetectable PCT was found in five non-MTC patients with false positive CT results.ConclusionsElecsys® PCT assay is a highly sensitive and specific alternative MTC marker. At the very least it appears useful in patients with positive CT results as negative PCT values securely exclude active MTC. The availability of both markers on the same automated platform facilitates reflex or reflective strategies to refine the laboratory diagnosis.

show abstract

Analytical Quality of Calcitonin Determination and Its Effect on the Adequacy of Screening for Medullary Carcinoma of the Thyroid

Cited by 21 publications

References 4 publications

Stability of synthetic cathinones in clinical and forensic toxicological analysis—Where are we now?

Stability of synthetic cathinones in clinical and forensic toxicological analysis—Where are we now?

Calcitonin screening in patients with thyroid nodules

Clinical performance of calcitonin and procalcitonin Elecsys^® immunoassays in patients with medullary thyroid carcinoma

Contact Info

Product

Resources

About

Analytical Quality of Calcitonin Determination and Its Effect on the Adequacy of Screening for Medullary Carcinoma of the Thyroid

Cited by 21 publications

References 4 publications

Stability of synthetic cathinones in clinical and forensic toxicological analysis—Where are we now?

Stability of synthetic cathinones in clinical and forensic toxicological analysis—Where are we now?

Calcitonin screening in patients with thyroid nodules

Clinical performance of calcitonin and procalcitonin Elecsys® immunoassays in patients with medullary thyroid carcinoma

Contact Info

Product

Resources

About

Clinical performance of calcitonin and procalcitonin Elecsys^® immunoassays in patients with medullary thyroid carcinoma