Construction and Characterization of a Chitosan-Immobilized-Enzyme and β-Cyclodextrin-Included-Ferrocene-Based Electrochemical Biosensor for H2O2 Detection

Abstract

An electrochemical detection biosensor was prepared with the chitosan-immobilized-enzyme (CTS-CAT) and β-cyclodextrin-included-ferrocene (β-CD-FE) complex for the determination of H2O2. Ferrocene (FE) was included in β-cyclodextrin (β-CD) to increase its stability. The structure of the β-CD-FE was characterized. The inclusion amount, inclusion rate, and electrochemical properties of inclusion complexes were determined to optimize the reaction conditions for the inclusion. CTS-CAT was prepared by a step-by-step immobilization method, which overcame the disadvantages of the conventional preparation methods. The immobilization conditions were optimized to obtain the desired enzyme activity. CTS-CAT/β-CD-FE composite electrodes were prepared by compositing the CTS-CAT with the β-CD-FE complex on a glassy carbon electrode and used for the electrochemical detection of H2O2. It was found that the CTS-CAT could produce a strong reduction peak current in response to H2O2 and the β-CD-FE could amplify the current signal. The peak current exhibited a linear relationship with the H2O2 concentration in the range of 1.0 × 10−7–6.0 × 10−3 mol/L. Our work provided a novel method for the construction of electrochemical biosensors with a fast response, good stability, high sensitivity, and a wide linear response range based on the composite of chitosan and cyclodextrin.

Sensitive Detection of Serum Creatinine Based on β-Cyclodextrin-Ferrocenylmethanol Modified Screen-printed Electrode

“…So the β-CD-Fc-OH/SPE is more suitable than the Fc-OH/SPE to detect creatinine concentration. The sensor is consistent with Dong's study, 13 which improved the sensitivity of detecting H2O2 by introducing β-CD. Secondly, verify the difference of sensitivity from another perspective between the β-CD-Fc-OH/SPE and Fc-OH/SPE.…”

“…11 As is well known, β-CD can increase the solubility and stability of Fc to prevent Fc loss. 12,13 But there are few reports on the application of β-CD-Fc-OH in the detection of creatinine.…”

Immobilization of endoglucanase Cel9A on chitosan nanoparticles leads to its stabilization against organic solvents: the use of polyols to improve the stability

“…The availability of chitosan and its unique chemical and biological properties such as hydrophilicity, nontoxicity, biocompatibility, and biodegradability make it a very attractive biomaterial for enzyme immobilization (Chen et al 2013;Krajewska 2004;Rampino et al 2013). Immobilization of enzyme on chitosan support provides an appropriate microenvironment for the enzymatic reaction where enzyme reactivity is well preserved against different solvents (Dong et al 2017). Nanoparticles have unique characteristics due to their small size and large surface area to volume ratio, which makes them ideal for the enzyme immobilization (Ansari and Husain 2012).…”

Chitin/Chitosan: Versatile Ecological, Industrial, and Biomedical Applications

“…Teepoo et al (2017) constructed an electrochemical biosensor to detect and monitor hydrogen peroxide by using horseradish peroxidase immobilized on a chitin-gelatin nanofiber composite. Another biosensor for hydrogen peroxide that used immobilized catalase on chitosan-β-cyclodextrin (with ferrocene in its cavity) was fabricated by Dong et al (2017). It was based on chitosan-functionalized graphene oxide (enriched with carboxylic moieties)-polypyrrole nanocomposite able to electrocatalytically reduce hydrogen peroxide.…”