Constructing an anti-S. mutans and mineralizing membrane by combination self-assembled lysozyme with antimicrobial peptide

Fang, Zehui; Zhang, Yuyuan; Cao, Chris Ying; Li, Quan Li; Wong, Hai Ming

doi:10.1016/j.matdes.2022.110891

Cited by 7 publications

(3 citation statements)

References 56 publications

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“…Furthermore, a dual-purpose membrane, amalgamating lysozyme with polyphemusin I (PI), aimed to suppress S. mutans and bolster tooth remineralization. 312 Enamel treated with lysozyme demonstrated enhanced PI absorption, resulting in more effective S. mutans regulation and biofilm reduction. Such PI-coated enamel safeguarded against calcium depletion but also exhibited superior crystal reformation and a substantial decrease in lesion depth (72.45 AE 4.07 mm) in comparison with the uncoated variant (93.30 AE 7.64 mm) post S. mutans-induced demineralization.…”

Section: Cell or Tissue Engineeringmentioning

confidence: 97%

Protein-based bioactive coatings: from nanoarchitectonics to applications

Fu,

Wang,

Zhou

et al. 2024

Chem. Soc. Rev.

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Section: Cell or Tissue Engineeringmentioning

confidence: 97%

Protein-based bioactive coatings: from nanoarchitectonics to applications

Fu,

Wang,

Zhou

et al. 2024

Chem. Soc. Rev.

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“…Nanofilms offer unique advantages for the delivery of lysozyme in wound healing due to their engineered structure (Fang et al, 2022). Their molecular-level precision allows for controlled and sustained release of the enzyme, providing a steady therapeutic effect.…”

Section: Nanofilmsmentioning

confidence: 99%

Exploring the therapeutic potential of recombinant human lysozyme: a review on wound management system with antibacterial

Zhao,

Huang,

2023

Front. Bioeng. Biotechnol.

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Lysozyme, a natural antibacterial enzyme protein, possesses the ability to dissolve the cell walls of Gram-positive bacteria, demonstrating broad-spectrum antibacterial activity. Despite its significant potential in treating wound infections and promoting wound healing, its widespread clinical application has yet to be realized. Current research is primarily focused on carrier-based delivery systems for lysozyme. In this review, we discuss four delivery systems that can be employed for lysozyme in wound healing treatment, specifically hydrogels, nanofilms, electrospun fibrous membranes, and modified-lysozyme composite systems. These systems not only enhance the stability of lysozyme but also enable its controlled and sustained release at wound sites, potentially overcoming some of the challenges associated with its direct application. Lastly, we delve into the perspectives and challenges related to the use of these delivery systems, hoping to spur further research and innovation in this promising field.

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“…Moreover, lysozyme can inactivate a layer of non-antigenic glycosaminoglycans in tumour cells and thus inhibit their growth [ 6 , 7 , 8 , 9 , 10 ]. In addition to antimicrobial action, lysozyme hydrochloride serves as a protective agent against caries by violating the ability of microorganisms to be attached to a tooth surface [ 11 ] and has anti-inflammatory and immunomodulating effects [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

The Study of the Cytotoxicity, Proliferative and Microbiological Activity of the Medicated Chewing Gum with Ascorbic Acid and Lysozyme Hydrochloride Using Different Culture of Cells

et al. 2023

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Medicated chewing gum with lysozyme hydrochloride and ascorbic acid as active pharmaceutical ingredients was developed for application in dentistry. The aim of this research was to study the cytotoxicity, proliferative, and microbiological activities of the active ingredients in different types of cell cultures. The preclinical study of active pharmaceutical ingredients and their combinations was carried out using culture lines such as HepG2 (human hepatocarcinoma cells), Hek293 (human embryonic kidney cells), and MAEC (mouse aortic endothelial cells). MTT assays were used to analyse cytotoxicity and proliferative activity, while the state of antioxidant protection was assessed by the content of sulfhydryl groups and catalase activity. The determination of lipid peroxidation products was based on the level of TBA-active products. As a microbiological model for studying the effect of the developed dental medicine on the ability of the oral cavity microorganisms to form biofilms, the following strains were used: Streptococcus mutans, Staphylococcus aureus, Staphylococcus epidermidis, Lactobacillus plantarum, and Candida albicans. The optical density of the formed biofilm was evaluated by the intensity of the experimental sample’s colour on a StatFax 303 Plus photometer at a wavelength of 630 nm. The combination of ascorbic acid and lysozyme hydrochloride in the established concentrations (20 mg and 10 mg per 1 gum, respectively) resulted in a slight stimulation of cell proliferation without any toxic effects and increased antioxidant protection, preventing the development of oxidative stress. It was found that, in contrast to the separately used active substances, the combination of lysozyme hydrochloride and ascorbic acid inhibits the biofilm formation of all studied microorganisms and shows the ability to destroy diurnal biofilms of L. plantarum and fungi of the genus Candida, indicating potentiation and summation of the active pharmaceutical ingredients’ composition effects in the developed dental medicine. Due to the observed positive pharmacological and microbiological action, the combination of lysozyme hydrochloride and ascorbic acid in the medicated chewing gum serves as a promising tool for the prevention and treatment of infectious and inflammatory diseases of the periodontium and mucous membranes and the prevention of caries.

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Constructing an anti-S. mutans and mineralizing membrane by combination self-assembled lysozyme with antimicrobial peptide

Cited by 7 publications

References 56 publications

Protein-based bioactive coatings: from nanoarchitectonics to applications

Protein-based bioactive coatings: from nanoarchitectonics to applications

Exploring the therapeutic potential of recombinant human lysozyme: a review on wound management system with antibacterial

The Study of the Cytotoxicity, Proliferative and Microbiological Activity of the Medicated Chewing Gum with Ascorbic Acid and Lysozyme Hydrochloride Using Different Culture of Cells

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