Constrained and UV-activatable cell-penetrating peptides for intracellular delivery of liposomes

Hansen, Morten Borre; Gaal, Ethlinn V.B. van; Minten, Inge J.; Storm, Gert; Hest, Jan C. M. van; Löwik, Dennis W. P. M.

doi:10.1016/j.jconrel.2012.10.008

Cited by 68 publications

(52 citation statements)

References 75 publications

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“…However, when the drug was covalently entrapped in the micellar core, it circulated together with the PM (Fig. 5B), and via its substantially prolonged circulation time, achieved much higher concentration in tumors as compared to free DMS or DMS physically entrapped in core-crosslinked PM, respectively [61].…”

Section: Covalent Drug Incorporationmentioning

confidence: 99%

Polymeric micelles for cancer therapy: 3 C’s to enhance efficacy

Talelli

Rijcken

Hennink

et al. 2012

Current Opinion in Solid State and Materials Science

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Section: Covalent Drug Incorporationmentioning

confidence: 99%

Polymeric micelles for cancer therapy: 3 C’s to enhance efficacy

Talelli

Rijcken

Hennink

et al. 2012

Current Opinion in Solid State and Materials Science

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“…Hansen et al proposed a liposomal drug delivery system with constrained cell penetrating peptides (TAT) for intracellular delivery [48]. TAT was incorporated into a loop on the surface of PEGylated liposomes via two alkyl-chains, one of which contained a UV-cleavable linker.…”

Section: Stimuli-responsive Steric Protections Of Cppsmentioning

confidence: 99%

Taming Cell Penetrating Peptides: Never Too Old To Teach Old Dogs New Tricks

Zhang

Gao

2015

Mol. Pharmaceutics

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Cell penetrating peptides (CPPs) have received substantial attention due to their intrinsic property to cross plasma membranes or even as helpers to facilitate the cellular entry of drug molecules, macromolecules, and nanoparticles. Although CPPs and CPP-like peptides provided versatile platforms for drug delivery, their nonselectivity or lack of delivery efficiency is stirring up debates as to the tactics for the optimizing the CPPs themselves. The good news is that, as spurred by the recent progress in the understanding of tumor microenvironment as well as biochemistry and material sciences, we have made attempts in working on perfecting or even "taming" CPPs and CPP-functionalized drug vectors for tumor delivery, and some of them afforded gratifying results. Due to the fact that these peptides are mainly short peptides made up of amino acids (5-30 amino acids), the addition, modification, or replacement of amino acids might lead to surprisingly improved performance. Several novel environment-responsive CPPs or CPP-like peptides have also been discovered. In this review we will discuss the measures taken to harness the power of CPPs and the discovery of environment-responsive peptides with CPP properties.

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“…Owing to these unique properties, the CPPs could interact with the negatively charged cellular membrane and enter the cells by means of their cell permeable characteristics without any cytotoxicty [16]. For over 20 years, CPP based gene delivery has been investigated [17–23] to enhance the transfection efficiency and introduce the targeting capability to the CPP/DNA complexes. The first CPPs were truncated from the transduction domain of the HIV-virus, TAT (48–60), since then it has been widely studied [24].…”

Section: Introductionmentioning

confidence: 99%

A branched TAT cell-penetrating peptide as a novel delivery carrier for the efficient gene transfection

et al. 2016

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BackgroundCell penetrating peptides (CPPs) as one class of non-viral vectors, have been widely explored as a delivery tool due to their cell-penetrating capability with low cytotoxicity. However, CPPs have reported to have low gene transfection efficiency mainly due to the fact that DNA is larger than other biomolecules. On the other hand, the conventional linear CPPs are unstable for constructing the DNA complexes with it. Thus, here we designed a branched CPP using disulfide bridges based on the linear TAT peptide, to enhance the gene delivery efficiency in a better way.ResultsThe branched TAT (BTAT) was synthesized by the DMSO oxidation method and showed high-molecular-weight about 294 kDa. The resulting BTAT was complexed with plasmid green fluorescence protein (pGFP) gene at various N/P ratios. The gene transfection efficiency was assessed on HeLa cells after treating with BTAT/pGFP complexes, showed high gene transfection efficiency as conformed by flowcytometry followed by confocal laser scanning microscopy (CLSM) visualization.ConclusionThe novel BTAT/pGFP complex exhibited significantly higher stability and redox cleavability by reducing agent. In addition, BTAT showed higher transfection efficiency approximately 40-fold than those of the TAT and mTAT complexes. Our primary experiments demonstrated the potential of BTAT as a suitable candidate for gene delivery and it could be applied for various types of gene delivery platforms.

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Constrained and UV-activatable cell-penetrating peptides for intracellular delivery of liposomes

Cited by 68 publications

References 75 publications

Polymeric micelles for cancer therapy: 3 C’s to enhance efficacy

Polymeric micelles for cancer therapy: 3 C’s to enhance efficacy

Taming Cell Penetrating Peptides: Never Too Old To Teach Old Dogs New Tricks

A branched TAT cell-penetrating peptide as a novel delivery carrier for the efficient gene transfection

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