Constitutive α-amylase producing mutant and recombinant haploid strains of thermophilic fungusThermomyces lanuginosus

Abstract

Morphological, developmental and antimetabolite-resistant mutants of T. lanuginosus were characterized and used for screening with the aim to develop constitutive alpha-amylase-hyperproducing strains. The protoplast fusion of two spontaneous mutants of T. lanuginosus, characterized as asporulating and resistant to 2-deoxy-D-glucose (2DG), resulted in sporulating, 2DG sensitive heterokaryotic fusants. A recombinant haploid strain F64fB developed there from produced alpha-amylase constitutively in glucose-containing medium. Constitutive alpha-amylase-hyperproducing mutant (III8) obtained after cyclic mutagenesis and screening yielded approximately 20 fold more alpha-amylase in a glycerol-containing medium than the wild strain.

Characterization and Optimization of Amylase Producing Bacteria Isolated from Solid Waste

“…Although they can be isolated from different source like animal, plant and microorganism, the microbial amylases are more thermostable and have higher yield [5]. Diverse fungal strains are able to produce amylase both intracellularly and extracellularly depending upon the fermentation process [6][7][8] but bacteria are the preferred source not only because maximum amount of generated enzyme within a very short time period but also due to its extracellular production attribute, which are indeed, easy to isolate [9][10][11]. Hence, bacterial amylases can provide an exquisite alternative to the chemical hydrolysis of starch [12].…”

Evaluation of secretome of highly efficient lignocellulolytic Penicillium sp. Dal 5 isolated from rhizosphere of conifers

“…Macroscopic studies focused on appearance, color texture and other physical features of colony on PDA medium. Whereas, microscopic examination was done to study arrangement of phialides and spores produced employing slide culturing technique (Chadha et al, 2005). The molecular characterization of isolates was carried out on the basis of rDNA sequence amplified using universal primers ITS1 (5'CCGTAGGTGAACCTGCGG-3') and ITS4 (5'-TCCTCCGCTTATTGATATG-3') (White et al, 1990).…”

Physical and Chemical Mutation for Enhanced Alpha-Amylase Production by Aspergillus fumigatus NTCC1222 under Solid State Fermentation Conditions Using Agri-Residue Waste

“…Energy, Environment and Economy [9]. Promising results through random mutagenesis thus has encouraged the research in this area [6,[16][17][18][19]. Aspergillus fumigatus has already been shown to be a potential source of industrial enzymes; yet, the huge potential of the same is still to be fully explored [2,17,[20][21][22][23].…”

“…Promising results through random mutagenesis thus has encouraged the research in this area [6,[16][17][18][19]. Aspergillus fumigatus has already been shown to be a potential source of industrial enzymes; yet, the huge potential of the same is still to be fully explored [2,17,[20][21][22][23]. The aim of the present study was to enhance the industrial value of already explored as a promising source of amylase, Aspergillus fumigatus NTCC1222, in our laboratory [24][25][26][27].…”