Constitutive production of extracellular glucose isomerase by an osmophillic Aspergillus sp. under submerged conditions

Sayyed, R. Z.; Shimpi, G. B.; Chincholkar, S. B.

doi:10.1007/s13197-010-0084-3

Cited by 5 publications

(4 citation statements)

References 17 publications

(16 reference statements)

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“…Glucose/xylose isomerase reversely converts D-glucose and D-Xylose into D-fructose and D-Xylulose, respectively [148,149]. Though glucose/xylose isomerase was extensively reported from many bacteria as Bacillus licheniformis [147], Serratia marcescens [150], Caldicellulosiruptor bescii [151], and Streptomyces lividans [152], the fungal enzyme was reported from a few species of Aspergillus genus [153]. Marshall and Kooi reported for the first time the ability of xylose isomerase from Pseudomonas hydrophila to convert D-glucose into D-fructose [154].…”

Section: Isomerases (Ec 5)mentioning

confidence: 99%

A Comprehensive Insight into Fungal Enzymes: Structure, Classification, and Their Role in Mankind’s Challenges

El‐Gendi

Saleh

Badierah

et al. 2021

JoF

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Enzymes have played a crucial role in mankind’s challenges to use different types of biological systems for a diversity of applications. They are proteins that break down and convert complicated compounds to produce simple products. Fungal enzymes are compatible, efficient, and proper products for many uses in medicinal requests, industrial processing, bioremediation purposes, and agricultural applications. Fungal enzymes have appropriate stability to give manufactured products suitable shelf life, affordable cost, and approved demands. Fungal enzymes have been used from ancient times to today in many industries, including baking, brewing, cheese making, antibiotics production, and commodities manufacturing, such as linen and leather. Furthermore, they also are used in other fields such as paper production, detergent, the textile industry, and in drinks and food technology in products manufacturing ranging from tea and coffee to fruit juice and wine. Recently, fungi have been used for the production of more than 50% of the needed enzymes. Fungi can produce different types of enzymes extracellularly, which gives a great chance for producing in large amounts with low cost and easy viability in purified forms using simple purification methods. In the present review, a comprehensive trial has been advanced to elaborate on the different types and structures of fungal enzymes as well as the current status of the uses of fungal enzymes in various applications.

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Section: Isomerases (Ec 5)mentioning

confidence: 99%

A Comprehensive Insight into Fungal Enzymes: Structure, Classification, and Their Role in Mankind’s Challenges

El‐Gendi

Saleh

Badierah

et al. 2021

JoF

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“…Conversion of glucose to fructose has not been described in yeasts so far but many bacteria and a few fungal species conduct this direct conversion using a glucose isomerase, an enzyme of exceptional biotechnological interest (Bhosale et al, 1996). Examples in fungi are found in species belonging to the genera Piromyces (Lee et al, 2017), Aspergillus (Sayyed et al, 2010) and Penicillium (Kathiresan and Manivannan, 2006). We searched for the presence of a glucose isomerase in the St. bombicola genome and the genomes of other W/S-clade species using fungal and bacterial glucose isomerases as queries in tBLASTx searches (AJ249909.1 and FJ858195.1, respectively), but no candidate genes could be found ( e -value >1e –3 , Supplementary Figure S3).…”

Section: Resultsmentioning

confidence: 99%

“…For the detection of glucose isomerase activity, a protocol based on Sayyed et al (2010) was used. Cells of both the wild type St. bombicola and the mtdh1 Δ mtdh2 Δ mutant were cultivated for 24 or 76 h in YP supplemented with 20% (w/v) of glucose and cell-free extracts were obtained as described above.…”

Section: Methodsmentioning

confidence: 99%

A New Pathway for Mannitol Metabolism in Yeasts Suggests a Link to the Evolution of Alcoholic Fermentation

et al. 2019

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The yeasts belonging to the Wickerhamiella and Starmerella genera (W/S clade) share a distinctive evolutionary history marked by loss and subsequent reinstatement of alcoholic fermentation mediated by horizontal gene transfer events. Species in this clade also share unusual features of metabolism, namely the preference for fructose over glucose as carbon source, a rare trait known as fructophily. Here we show that fructose may be the preferred sugar in W/S-clade species because, unlike glucose, it can be converted directly to mannitol in a reaction with impact on redox balance. According to our results, mannitol is excreted to the growth medium in appreciable amounts along with other fermentation products such as glycerol and ethanol but unlike the latter metabolites mannitol production increases with temperature. We used comparative genomics to find genes involved in mannitol metabolism and established the mannitol biosynthesis pathway in W/S-clade species Starmerella bombicola using molecular genetics tools. Surprisingly, mannitol production seems to be so important that St. bombicola (and other W/S-clade species) deploys a novel pathway to mediate the conversion of glucose to fructose, thereby allowing cells to produce mannitol even when glucose is the sole carbon source. Using targeted mutations and 13C-labeled glucose followed by NMR analysis of end-products, we showed that the novel mannitol biosynthesis pathway involves fructose-6-phosphate as an intermediate, implying a key role for a yet unknown fructose-6-P phosphatase. We hypothesize that mannitol production contributed to mitigate the negative effects on redox balance of the ancient loss of alcoholic fermentation in the W/S clade. Presently, mannitol also seems to play a role in stress protection.

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“…217 Fungal isomerases are reported from a few species of Aspergillus genus. 218 This class of enzymes holds promise for both pharmaceutical and agrofood industries. 219,220…”

Section: Isomerasesmentioning

confidence: 99%

Role of fungal enzymes in the synthesis of pharmaceutically important scaffolds: a green approach

Kumar,

Narula,

Deswal

2023

Green Chem.

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Constitutive production of extracellular glucose isomerase by an osmophillic Aspergillus sp. under submerged conditions

Cited by 5 publications

References 17 publications

A Comprehensive Insight into Fungal Enzymes: Structure, Classification, and Their Role in Mankind’s Challenges

A Comprehensive Insight into Fungal Enzymes: Structure, Classification, and Their Role in Mankind’s Challenges

A New Pathway for Mannitol Metabolism in Yeasts Suggests a Link to the Evolution of Alcoholic Fermentation

Role of fungal enzymes in the synthesis of pharmaceutically important scaffolds: a green approach

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