Constitutive overexpression of the cytochrome P450 gene CYP6A1 in a house fly strain with metabolic resistance to insecticides

Cariño, Flerida A.; Koener, J. F.; Plapp, Frederick W.; Feyereisen, René

doi:10.1016/0965-1748(94)90034-5

Cited by 195 publications

(150 citation statements)

References 28 publications

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“…So far, all other described P450s associated with insecticide resistance are expressed in susceptible insects and overexpressed in resistant insects (16,17,29,30,39,41,42,44,45,(52)(53)(54)(55)(56)(57). Overexpression can be caused by trans-(52) or cis-acting factors, the latter often associated with transposable element insertion in the promoter region (53,58,59).…”

Section: Discussionmentioning

confidence: 99%

Resistance of AustralianHelicoverpa armigerato fenvalerate is due to the chimeric P450 enzyme CYP337B3

Joußen¹,

Agnolet

Lorenz

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

177

199

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Worldwide, increasing numbers of insects have evolved resistance to a wide range of pesticides, which hampers their control in the field and, therefore, threatens agriculture. Members of the carboxylesterase and cytochrome P450 monooxygenase superfamilies are prominent candidates to confer metabolic resistance to pyrethroid insecticides. Both carboxylesterases and P450 enzymes have been shown to be involved in pyrethroid resistance in Australian Helicoverpa armigera, the noctuid species possessing by far the most reported resistance cases worldwide. However, specific enzymes responsible for pyrethroid resistance in field populations of this species have not yet been identified. Here, we show that the resistance toward fenvalerate in an Australian strain of H. armigera is due to a unique P450 enzyme, CYP337B3, which arose from unequal crossing-over between two parental P450 genes, resulting in a chimeric enzyme. CYP337B3 is capable of metabolizing fenvalerate into 4′-hydroxyfenvalerate, which exhibits no toxic effect on susceptible larvae; enzymes from the parental P450 genes showed no detectable fenvalerate metabolism. Furthermore, a polymorphic H. armigera strain could be bred into a susceptible line possessing the parental genes CYP337B1 and CYP337B2 and a resistant line possessing only CYP337B3. The exclusive presence of CYP337B3 in resistant insects of this strain confers a 42-fold resistance to fenvalerate. Thus, in addition to previously documented genetic mechanisms of resistance, recombination can also generate selectively advantageous variants, such as this chimeric P450 enzyme with an altered substrate specificity leading to a potent resistance mechanism.pest management | cotton bollworm O ne of the main threats of agriculture nowadays is the rapid development of resistance of pest insect species to control agents worldwide. The cotton bollworm, Helicoverpa armigera (Hübner), is the noctuid species possessing by far the most reported cases of insecticide resistance worldwide with evolved resistance against pyrethroids, organophosphates, carbamates, organochlorines (www.pesticideresistance.org), and recently against the macrocyclic lactone spinosad (1) and Bacillus thuringiensis-derived toxins (2). This capacity is partly due to its distribution, which is one of the widest for any agricultural insect pest species, covering Africa, the Middle East, southern Europe, India, central and southeastern Asia, eastern and northern Australia, New Zealand, and many eastern Pacific Islands (3). In addition, H. armigera is a significant pest of cotton, the single crop most intensively sprayed with insecticides. Almost 30% of all pesticides used worldwide are directed against this insect pest (4). In Australia, the economic losses due to direct yield reduction and pest management of H. armigera and endemic H. punctigera were estimated to be approximately A$150 million in 1990-1991 (5). In addition, H. armigera is highly polyphagous, feeding on 72 known host plant species distributed in 29 families in Australia (6).Af...

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Section: Discussionmentioning

confidence: 99%

Resistance of AustralianHelicoverpa armigerato fenvalerate is due to the chimeric P450 enzyme CYP337B3

Joußen¹,

Agnolet

Lorenz

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

177

199

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“…In many cases, the overexpression of P450 genes results in increased levels of total P450s and their activities are responsible for insecticide resistance (Cariño et al 1994;Zhu et al 2008b). Both constitutively increased expression (overexpression) and induction of P450s are thought to be responsible for increased levels of detoxification of insecticides (Pavek & Dvorak 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Down-or up-regulation of P450 gene expression can significantly affect the disposition of xenobiotics or endogenous compounds in the tissues of organisms, and thus alter their pharmacological/toxicological effects (Liu et al 2011b). In arthropods, an increase in P450 activity is associated with the enhanced metabolic detoxification of insecticides; and a constitutive over expression of P450 genes has been implicated in the evolution of resistance to insecticides (Cariño et al 1994;Feyereisen 2005;Zhu et al 2008a) and tolerance to plant toxins (Wen et al 2003). The isolation, characterization, classification and nomenclature of specific insect P450s are critical first steps towards understanding their involvement in these important metabolic processes.…”

mentioning

confidence: 99%

Mediation of Pyrethroid Insecticide Toxicity to Honey Bees (Hymenoptera: Apidae) by Cytochrome P450 Monooxygenases

Johnson¹,

Wen²,

Schuler³

et al. 2006

Journal of Economic Entomology

138

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“…Members of the cluster may be expressed as multiple (up to five) alleles (33). Enhanced levels of oxidases in resistant insects result from constitutive overexpression rather than amplification (34,35). The mechanisms of oxidase overproduction in resistance are under extensive investigation and appear to result from both cisand trans-acting factors, perhaps associated with the phenomenon of induction (36)(37)(38).…”

Section: B Amentioning

confidence: 99%

Insecticide Resistance and Vector Control

Brogdon

McAllister²

1999

Journal of Agromedicine

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Constitutive overexpression of the cytochrome P450 gene CYP6A1 in a house fly strain with metabolic resistance to insecticides

Cited by 195 publications

References 28 publications

Resistance of AustralianHelicoverpa armigerato fenvalerate is due to the chimeric P450 enzyme CYP337B3

Resistance of AustralianHelicoverpa armigerato fenvalerate is due to the chimeric P450 enzyme CYP337B3

Mediation of Pyrethroid Insecticide Toxicity to Honey Bees (Hymenoptera: Apidae) by Cytochrome P450 Monooxygenases

Insecticide Resistance and Vector Control

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