Constitutive overexpression of cytochrome <scp>P450</scp> associated with imidacloprid resistance in <i>Laodelphax striatellus</i> (Fallén)

Elzaki, Mohammed Esmail Abdalla; Zhang, Wanfang; Ai, Feng; Qiou, Xiaoyan; Zhao, Wenqing; Han, Zhaojun

doi:10.1002/ps.4155

Cited by 56 publications

(70 citation statements)

References 34 publications

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“…In this study, the expression level of CYP3356A1 was significantly induced by imidacloprid, thiamethoxam, and β‐cypermethrin, although no statistical difference was found after chlorpyrifos exposure. It is reported that the enhanced transcript level of CYP353D1v2 was correlated with imidacloprid resistance in Laodelphax striatellus . Overexpression of detoxification‐related P450 was related to thiamethoxam resistance in Aphis gossypii Glover .…”

Section: Discussionmentioning

confidence: 98%

Identification of a novel cytochrome P450 CYP3356A1 linked with insecticide detoxification in Bradysia odoriphaga

Chen

Shan

Liu

et al. 2018

Pest Management Science

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Section: Discussionmentioning

confidence: 98%

Identification of a novel cytochrome P450 CYP3356A1 linked with insecticide detoxification in Bradysia odoriphaga

Chen

Shan

Liu

et al. 2018

Pest Management Science

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“…For instance, through RNAi studies, several studies have shown that constitutive upregulation of CYPs mediates resistance to pyrethroid insecticides in the diamondback moth, Plutella xylostella 35 , the carmine spider mite, Tetranychus cinnabarinus 36 , and the red flour beetle, Tribolium castaneum 37 . Additionally, CYPs are involved in resistance to neonicotinoids in the small brown plant hopper, Laodelphax striatellus 38 and the tobacco whitefly, B. tabaci 39 , to organophosphates in the tobacco cutworm, Spodoptera litura 40 , and to abamectin in P . xylostella 41 .…”

Section: Discussionmentioning

confidence: 99%

Overexpression of a cytochrome P450 and a UDP-glycosyltransferase is associated with imidacloprid resistance in the Colorado potato beetle, Leptinotarsa decemlineata

Kaplanoglu

Chapman

Scott

et al. 2017

Sci Rep

113

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Current control of insect pests relies on chemical insecticides, however, insecticide resistance development by pests is a growing concern in pest management. The main mechanisms for insecticide resistance typically involve elevated activity of detoxifying enzymes and xenobiotic transporters that break-down and excrete insecticide molecules. In this study, we investigated the molecular mechanisms of imidacloprid resistance in the Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae), an insect pest notorious for its capacity to develop insecticide resistance rapidly. We compared the transcriptome profiles of imidacloprid-resistant and sensitive beetle strains and identified 102 differentially expressed transcripts encoding detoxifying enzymes and xenobiotic transporters. Of these, 74 were up-regulated and 28 were down-regulated in the resistant strain. We then used RNA interference to knock down the transcript levels of seven up-regulated genes in the resistant beetles. Ingestion of double-stranded RNA successfully knocked down the expression of the genes for three cytochrome P450s (CYP6BQ15, CYP4Q3 and CYP4Q7), one ATP binding cassette (ABC) transporter (ABC-G), one esterase (EST1), and two UDP-glycosyltransferases (UGT1 and UGT2). Further, we demonstrated that silencing of CYP4Q3 and UGT2 significantly increased susceptibility of resistant beetles to imidacloprid, indicating that overexpression of these two genes contributes to imidacloprid resistance in this resistant strain.

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“…Generally, multiple overexpressed P450 genes are responsible for high levels of insecticide by resistance in insects, such as the co‐upregulation of multiple P450 genes in permethrin‐resistant house flies (Zhu et al ., ) and Culex mosquitoes (Liu et al ., ; Yang & Liu, ). Here, CYP6AY3v2 was also co‐upregulated with CYP4C71v2 , CYP4C72 and CYP353D1v2 in the L. striatellus imidacloprid‐resistant strain Imid‐R (Elzaki et al ., ). CYP4C71v2 was reactive against imidacloprid, as there was apparent substrate depletion observed in in vitro metabolism assays (unpublished data).…”

Section: Discussionmentioning

confidence: 97%

“…() indicated that piperonyl butoxide had significant synergistic effects on three imidacloprid‐resistant L. striatellus populations. A previous study by our laboratory also showed that the expression level of a cytochrome P450 gene CYP6AY3v2 was significantly upregulated in a L. striatellus imidacloprid‐resistant strain (Imid‐R; Elzaki et al ., ). In this study, a transgenic Drosophila melanogaster line that overexpressed the L. striatellus CYP6AY3v2 gene was established to validate metabolism‐based imidacloprid resistance, and CYP6AY3v2 was functionally expressed in Spodoptera frugiperda 9 (SF9) cells to examine its metabolizing ability of imidacloprid.…”

Section: Introductionmentioning

confidence: 97%

Imidacloprid is hydroxylated by Laodelphax striatellus CYP6AY3v2

Wang

Zhu

Deng

et al. 2017

Insect Molecular Biology

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Laodelphax striatellus (Fallén) is one of the most destructive pests of rice, and has developed high resistance to imidacloprid. Our previous work indicated a strong association between imidacloprid resistance and the overexpression of a cytochrome P450 gene CYP6AY3v2 in a L. striatellus imidacloprid resistant strain (Imid-R). In this study, a transgenic Drosophila melanogaster line that overexpressed the L. striatellus CYP6AY3v2 gene was established and was found to confer increased levels of imidacloprid resistance. Furthermore, CYP6AY3v2 was co-expressed with D. melanogaster cytochrome P450 reductase (CPR) in Spodoptera frugiperda 9 (SF9) cells. A carbon monoxide difference spectra analysis indicated that CYP6AY3v2 was expressed predominately in its cytochrome P450 (P450) form, which is indicative of a good-quality functional enzyme. The recombinant CYP6AY3v2 protein efficiently catalysed the model substrate P-nitroanisole to p-nitrophenol with a maximum velocity (V ) of 60.78 ± 3.93 optical density (mOD)/min/mg protein. In addition, imidacloprid itself was metabolized by the recombinant CYP6AY3v2/nicotinamide adenine dinucleotide 2'-phosphate reduced tetrasodium salt (NADPH) CPR microsomes in in vitro assays (catalytic constant (K ) = 0.34 pmol/min/pmol P450, michaelis constant (K ) = 41.98 μM), and imidacloprid depletion and metabolite peak formation were with a time dependence. The data provided direct evidence that CYP6AY3v2 is capable of hydroxylation of imidacloprid and conferring metabolic resistance in L. striatellus.

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Constitutive overexpression of cytochrome P450 associated with imidacloprid resistance in Laodelphax striatellus (Fallén)

Abstract: Constitutive overexpression of four CYP genes was associated with imidacloprid resistance in long-term selection, and expression of CYP353D1v2 with imidacloprid resistance in short-term selection in L. striatellus.

Cited by 56 publications

References 34 publications

Identification of a novel cytochrome P450 CYP3356A1 linked with insecticide detoxification in Bradysia odoriphaga

Identification of a novel cytochrome P450 CYP3356A1 linked with insecticide detoxification in Bradysia odoriphaga

Overexpression of a cytochrome P450 and a UDP-glycosyltransferase is associated with imidacloprid resistance in the Colorado potato beetle, Leptinotarsa decemlineata

Imidacloprid is hydroxylated by Laodelphax striatellus CYP6AY3v2

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