Constitutive expression and activity of cytochrome P450 in conventional pigs

Nielsen, S. B.; Bauhaus, Yvonne; Zamaratskaia, Galia; Junqueira, Matheus Antunes; Blaabjerg, K.; Petrat-Melin, Bjørn; Young, Jette F.; Rasmussen, Martin Krøyer

doi:10.1016/j.rvsc.2016.12.003

Cited by 23 publications

(19 citation statements)

References 42 publications

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“…Both CYP2A19 and CYP2E1 have the highest abundance in the hepatic tissue of adult pigs, and their constitutive mRNA expression and activities are similar in the four major lobes of the porcine liver . CYP2A19 mRNA expression has been demonstrated in extrahepatic tissue, including kidney and adipose tissue, but not small intestine and skeletal muscle tissue . CYP2E1 mRNA expression has also been demonstrated in extrahepatic tissue, including the kidney, brain, lung and adipose tissue, but not the small intestine and skeletal muscle tissue .…”

Section: Tissue Distribution Hepatic and Extrahepatic Cyp2a19 And Cymentioning

confidence: 98%

“…Relative gene expression of CYP2A19 and CYP2E1 in tissues that contribute to detoxification processes in pig is summarised in Table . Both CYP2A19 and CYP2E1 have the highest abundance in the hepatic tissue of adult pigs, and their constitutive mRNA expression and activities are similar in the four major lobes of the porcine liver . CYP2A19 mRNA expression has been demonstrated in extrahepatic tissue, including kidney and adipose tissue, but not small intestine and skeletal muscle tissue .…”

Section: Tissue Distribution Hepatic and Extrahepatic Cyp2a19 And Cymentioning

confidence: 99%

“…Knowledge on the tissue distribution of the major CYPs is important in order to be able to fully interpret the response in CYP regulation following exposure to environmental and biological factors. The tissue distribution of CYP in pigs has been assessed 11,[13][14][15] and reviewed by Puccinelli et al 16 Previous studies have determined the CYP expression profile in both minipigs and conventional pigs. Relative gene expression of CYP2A19 and CYP2E1 in tissues that contribute to detoxification processes in pig is summarised in Table 2.…”

Section: Hepatic and Extrahepatic Cyp2a19 And Cyp2e1mentioning

confidence: 99%

“…17 CYP2A19 mRNA expression has been demonstrated in extrahepatic tissue, including kidney and adipose tissue, but not small intestine and skeletal muscle tissue. 11,15 CYP2E1 mRNA expression has also been demonstrated in extrahepatic tissue, including the kidney, brain, lung and adipose tissue, but not the small intestine and skeletal muscle tissue. 14,15 Although CYP2A19 and CYP2E1 mRNA expressions were observed in extrahepatic tissue, corresponding specific substrate metabolism was not demonstrated.…”

Section: Hepatic and Extrahepatic Cyp2a19 And Cyp2e1mentioning

confidence: 99%

“…11,15 CYP2E1 mRNA expression has also been demonstrated in extrahepatic tissue, including the kidney, brain, lung and adipose tissue, but not the small intestine and skeletal muscle tissue. 14,15 Although CYP2A19 and CYP2E1 mRNA expressions were observed in extrahepatic tissue, corresponding specific substrate metabolism was not demonstrated. 15 The constitutive expression of CYP2A19 and CYP2E1 is highly dependent on the weight and age of the pigs.…”

Section: Hepatic and Extrahepatic Cyp2a19 And Cyp2e1mentioning

confidence: 99%

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Porcine cytochrome 2A19 and 2E1

Burkina

Rasmussen

Oliinychenko

et al. 2018

Basic Clin Pharma Tox

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Cytochrome P450 (CYP) is a major group of enzymes, which conduct Phase I metabolism. Among commonly used animal models, the pig has been suggested as the most suitable model for investigating drug metabolism in human beings. Moreover, porcine CYP2A19 and CYP2E1 are responsible for the biotransformation of both endogenous and exogenous compounds such as 3-methylindole (skatole), sex hormones and food compounds. However, little is known about the regulation of porcine CYP2A19 and CYP2E1. In this MiniReview, we summarise the current knowledge about the regulation of porcine CYP2A19 and CYP2E1 by environmental, biological and dietary factors. Finally, we reflect on the need for further research, to clarify the interaction between active feed components and the porcine CYP system.

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Section: Tissue Distribution Hepatic and Extrahepatic Cyp2a19 And Cymentioning

confidence: 98%

Section: Tissue Distribution Hepatic and Extrahepatic Cyp2a19 And Cymentioning

confidence: 99%

Section: Hepatic and Extrahepatic Cyp2a19 And Cyp2e1mentioning

confidence: 99%

Section: Hepatic and Extrahepatic Cyp2a19 And Cyp2e1mentioning

confidence: 99%

Section: Hepatic and Extrahepatic Cyp2a19 And Cyp2e1mentioning

confidence: 99%

See 3 more Smart Citations

Porcine cytochrome 2A19 and 2E1

Burkina

Rasmussen

Oliinychenko

et al. 2018

Basic Clin Pharma Tox

Self Cite

View full text Add to dashboard Cite

show abstract

Porcine cytochrome P450 3A: current status on expression and regulation

Rasmussen

2020

Arch Toxicol

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Investigations on hepatic and intestinal drug-metabolizing cytochrome P450 enzymes in wild boar compared to domestic swine

et al. 2019

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Drug-metabolizing cytochrome P450 (CYP) enzymes are especially important in wild animals as they are directly exposed to environmental pollutants and bioactive molecules of plants. Our main goal was to monitor the activity of certain CYP enzymes in wild boar compared to domestic swine, and to assess various modulatory factors of xenobiotic biotransformation in wild boar. Liver and intestinal mucosa (duodenum, jejunum, ileum, caecum) samples were collected from 49 hunted free-range wild boars and 15 wild boar fetuses; domestic pig samples (n = 40) were gained from a slaughter house. Specific activity of CYP1A2, CYP2C9, and CYP3A4 enzymes was assessed by luminometric assays. The activity of hepatic CYP1A2 and CYP3A4 enzymes was significantly higher in wild boars than in domestic pigs, while CYP2C9-mediated hepatic metabolism was significantly less intense in wild boars than in pigs. Certain modulatory factors (sex, sexual maturation, and season) were also confirmed in wild boars. The activity of all investigated intestinal CYP enzymes remained under detection level in each gut section in both species. Hepatic CYP2C9 and CYP3A4 enzymes were measurable in wild boar fetuses, but their activity was remarkably lower than in adults. The described interspecies differences might be explained with the altered exposure of wild and domesticated animals to specific CYP modulators. As CYP enzymes in wild boars can be highly influenced by environmental pollutants, following further studies, they may serve as ecotoxicological markers of agricultural or industrial toxicants. Investigating CYP-related drug metabolism in wildlife species can clarify some toxicokinetic interactions, thus having huge importance in the production of safe game meat.

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Constitutive expression and activity of cytochrome P450 in conventional pigs

Cited by 23 publications

References 42 publications

Porcine cytochrome 2A19 and 2E1

Porcine cytochrome 2A19 and 2E1

Porcine cytochrome P450 3A: current status on expression and regulation

Investigations on hepatic and intestinal drug-metabolizing cytochrome P450 enzymes in wild boar compared to domestic swine

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