“…Total nitrogen (TN) and water-soluble nitrogen (WSN) were determined in quadruplicate for each kind of substrate using the Kjeldahl method according to Alais (1984). To determine the non-protein nitrogen (NPN) fraction, milk samples were mixed in Eppendorf tubes with a 240 g kg À1 trichloroacetic acid solution (TCA) to obtain a final TCA concentration equal to 120 g kg À1 .…”
“…Total nitrogen (TN) and water-soluble nitrogen (WSN) were determined in quadruplicate for each kind of substrate using the Kjeldahl method according to Alais (1984). To determine the non-protein nitrogen (NPN) fraction, milk samples were mixed in Eppendorf tubes with a 240 g kg À1 trichloroacetic acid solution (TCA) to obtain a final TCA concentration equal to 120 g kg À1 .…”
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