Isolation, Purification, and Analysis of Two κ-Casein-like Fractions from Sheep Casein

In order to study the race effect on the physico-chemical characteristics and protein profile, two sheep or ovine breeds Hamra and Ouled Djellal were raised in the same condition sat the ITELV (National institute of breeding) (Ain Hajar-Saida, Algeria). The analyses were focused on the pH, density, freezing, fat, total protein, dry defatted extract and total dry extract. The results obtained or these parameters showed no significant differences between the two races. Then the protein fractions were isolated and analyzed by Poly Acryl Amide Gel Electrophoresis (PAGE) under different conditions (native and in presence of urea). The resulting electrophoretic profiles have identified similarities between the two breeds milk.

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“…This is similar to observations reported by (Alais and Jollès, 1967;Chianese et al, 1992;Moatsou et al, 2004).…”

Section: Electrophoretic Analysis Of Total Caseinsupporting

confidence: 82%

CONTRIBUTION TO THE GENETIC DETERMINISM OF THE SHEEPâS MILK QUALITY. RACE INFLUENCE (HAMRA AND OULED DJELLAL) ON PHYSICO-CHEMICAL PARAMETERS AND PROTEIN PROFILE

Ameur

Fadela

Cheriguene

et al. 2014

OnLine Journal of Biological Sciences

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“…Sheep xa-casein was prepared according to Alais and Jollbs [8]. Trypsin and chymotrypsin were purchased from Worthington.…”

Section: Methodsmentioning

confidence: 99%

The Sequence of Sheep κ‐Casein: Primary Structure of para‐κ_A‐Casein

Jollès

Schoentgen

Hermann

et al. 1974

European Journal of Biochemistry

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The primary structure of sheep para-xA-casein, the N-terminal portion of x-casein, was established. The reduced alkylated protein was subjected to digestion with trypsin. The resulting soluble peptides were purified by a combination of Dowex 1 X2 chromatography and electrophoresis and chromatography on paper; the core peptides were solubilized in 50% formic acid and filtered on Sephadex G-50. The amino-acid sequence of these peptides was determined chiefly with a Sequencer.

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“…To test this hypothesis desialylated oligosaccharide chains present in the %-casein of ovine colostrum were isolated; establishment of their structure is rcported in this paper. Analytical data for the sugar content of x-casein from ewe's milk have been published previously [5,10,11].…”

mentioning

confidence: 99%

Structure of the Asialyl Oligosaccharide Chains of kappa-Casein Isolated from Ovine Colostrum

1980

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Three oligosaccharide alditols, a di (44–63%), tetra (32–46%), and a pentasacchariditol (5–10%) have been isolated from desialylated caseinomacropeptide obtained from ϰ‐casein of ewe colostrum. The disacchariditol is 2‐acetam‐do‐2‐deoxy‐3‐O‐(β‐D‐galactopyranosyl)galactitol; in the tetrasaccharide this unit is substituted in position 6 of the galactosaminitol moiety by 2‐acetamido‐2‐deoxy‐4‐O‐(β‐D‐galactopyranosyl)‐β‐D‐glucopyranose, while the pentasacchariditol is derived from the tetrasaccharide by addition of a D‐galactopyranosyl unit to position 3 of the galactopyranose unit substituting the glucosamine unit.

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Isolation, Purification, and Analysis of Two κ-Casein-like Fractions from Sheep Casein

Cited by 41 publications

References 20 publications

CONTRIBUTION TO THE GENETIC DETERMINISM OF THE SHEEPâS MILK QUALITY. RACE INFLUENCE (HAMRA AND OULED DJELLAL) ON PHYSICO-CHEMICAL PARAMETERS AND PROTEIN PROFILE

CONTRIBUTION TO THE GENETIC DETERMINISM OF THE SHEEPâS MILK QUALITY. RACE INFLUENCE (HAMRA AND OULED DJELLAL) ON PHYSICO-CHEMICAL PARAMETERS AND PROTEIN PROFILE

The Sequence of Sheep κ‐Casein: Primary Structure of para‐κ_A‐Casein

Structure of the Asialyl Oligosaccharide Chains of kappa-Casein Isolated from Ovine Colostrum

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Isolation, Purification, and Analysis of Two κ-Casein-like Fractions from Sheep Casein

Cited by 41 publications

References 20 publications

CONTRIBUTION TO THE GENETIC DETERMINISM OF THE SHEEPâS MILK QUALITY. RACE INFLUENCE (HAMRA AND OULED DJELLAL) ON PHYSICO-CHEMICAL PARAMETERS AND PROTEIN PROFILE

CONTRIBUTION TO THE GENETIC DETERMINISM OF THE SHEEPâS MILK QUALITY. RACE INFLUENCE (HAMRA AND OULED DJELLAL) ON PHYSICO-CHEMICAL PARAMETERS AND PROTEIN PROFILE

The Sequence of Sheep κ‐Casein: Primary Structure of para‐κA‐Casein

Structure of the Asialyl Oligosaccharide Chains of kappa-Casein Isolated from Ovine Colostrum

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Product

Resources

About

CONTRIBUTION TO THE GENETIC DETERMINISM OF THE SHEEPâS MILK QUALITY. RACE INFLUENCE (HAMRA AND OULED DJELLAL) ON PHYSICO-CHEMICAL PARAMETERS AND PROTEIN PROFILE

CONTRIBUTION TO THE GENETIC DETERMINISM OF THE SHEEPâS MILK QUALITY. RACE INFLUENCE (HAMRA AND OULED DJELLAL) ON PHYSICO-CHEMICAL PARAMETERS AND PROTEIN PROFILE

The Sequence of Sheep κ‐Casein: Primary Structure of para‐κ_A‐Casein