Constant specific growth rate in fed-batch cultivation of Bordetella pertussis using adaptive control

Soons, Zita; Voogt, Juliën; Straten, G. van; Boxtel, A.J.B. van

doi:10.1016/j.jbiotec.2006.03.005

Cited by 50 publications

(54 citation statements)

References 15 publications

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“…10) to investigate the effect of the off-gas dynamics during real batch and fed-batch experiments. The auto-tuning controller for specific growth rate, developed and validated for smallscale cultivations in [1,2], was applied to the pilot-scale cultivation with B. pertussis without any modification.…”

Section: Resultsmentioning

confidence: 99%

“…In the fed-batch phase of the cultivation the biomass more than doubles up to 3.5 OD by adding a feed with limiting substrates to control the specific growth rate. Higher biomass concentrations can be obtained, like in small-scale cultivations [1], if the headspace aeration were replaced by sparger aeration to obtain proper oxygen transfer.…”

Section: Discussionmentioning

confidence: 99%

“…In the fed-batch phase two 2.5 l concentrated stock solutions containing glutamate and lactate were added into the bioreactor to obtain higher biomass concentrations. The specific growth rate was controlled by an adaptive control law ( [1,2] and ''Appendix 1''). The tuning parameters of the specific growth rate controller c 1 and c 2 were adjusted until the performance measures were satisfactory using automatic tuning.…”

Section: Bioreactor Conditions and Analysismentioning

confidence: 99%

“…The control purpose is to regulate the specific growth rate (l) to a desired value by adding a feed with limiting substrates using the controller of [1]:…”

Section: Appendix 1: Monitoring and Controlmentioning

confidence: 99%

“…Changing from small to medium-or large-scale systems usually requires re-tuning of the controller settings. The applied adaptive controller was designed such that its settings are the result of the process characteristics [1] and it automatically tunes its settings if performance is poor [2]. In this work, we investigated the properties and performance of the specific growth rate controller in scale-up from laboratory to pilotscale.…”

Section: Introductionmentioning

confidence: 99%

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Scaling-up vaccine production: implementation aspects of a biomass growth observer and controller

Soons

IJssel²,

Pol³

et al. 2008

Bioprocess Biosyst Eng

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This study considers two aspects of the implementation of a biomass growth observer and specific growth rate controller in scale-up from small-to pilot-scale bioreactors towards a feasible bulk production process for whole-cell vaccine against whooping cough. The first is the calculation of the oxygen uptake rate, the starting point for online monitoring and control of biomass growth, taking into account the dynamics in the gas-phase. Mixing effects and delays are caused by amongst others the headspace and tubing to the analyzer. These gas phase dynamics are modelled using knowledge of the system in order to reconstruct oxygen consumption. The second aspect is to evaluate performance of the monitoring and control system with the required modifications of the oxygen consumption calculation on pilot-scale. In pilot-scale fed-batch cultivation good monitoring and control performance is obtained enabling a doubled concentration of bulk vaccine compared to standard batch production.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Bioreactor Conditions and Analysismentioning

confidence: 99%

“…The control purpose is to regulate the specific growth rate (l) to a desired value by adding a feed with limiting substrates using the controller of [1]:…”

Section: Appendix 1: Monitoring and Controlmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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