Consolidated bioprocessing of starch based on a bilayer cell factory without genetic modification of yeast

Drosos, Athanasios; Boura, Konstantina; Dima, Agapi; Karabagias, Ioannis K.; Nigam, Poonam Singh Nee; Κουτίνας, Αθανάσιος Α.

doi:10.1016/j.eti.2021.101844

Cited by 10 publications

(9 citation statements)

References 23 publications

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“…In other studies, porous supports, such as porous ceramic sinter, brick powder, diatomaceous earth, mineral kissiris, 𝛾-alumina pellets, gluten pellets, thin-shell silk cocoons, loofa sponge, orange peel, tubular, and bacterial cellulosic materials were used in fermentation processes with a mass greater than 20 g support L −1 . [7,[37][38][39][40][41][43][44]46] A comparison of ethanol yields obtained from various starchy raw materials using one-step SSF or two-step separate hydrolysis and fermentation (SHF) processes by selected biocatalysts without GMO yeasts is shown in Table 4. According to Table 3, researchers used combined biocatalysts based on yeasts and amylases to ferment starch, ethanol yield was ranged from 0.27 up to 0.49 g ethanol g -1 starch.…”

Section: Scientific and Technological Consideration Of Resultsmentioning

confidence: 99%

“…and a source of value-added products such as organic acids, bacterial cellulose, polymers, bioethanol, etc. [5,6] In addition, bioethanol produced mostly from plant biomass, such as starchy and cellulosic substrates [7,8] shows considerable promise because to its renewable nature. This alternative fuel has the potential to partially substitute fossil fuels and reduce the greenhouse effect by emitting low levels of carbon.…”

Section: Introductionmentioning

confidence: 99%

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Bacterial Nanocellulose‐Based Composite Biocatalysts for Starch‐to‐Bioethanol Valorization under Simultaneous Saccharification and Fermentation

Drosos,

Dima,

Kandylis

et al. 2023

Starch Stärke

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Composite biocatalysts (CB) consisting of amylases and Saccharomyces cerevisiae immobilized separately on bacterial nanocellulose (BNC) are used for the process of simultaneous saccharification and fermentation (SSF) of starch (5%, w/v) for bioethanol production. Parameters such as: i) addition of phosphates and/or divalent metal‐ions salts during the co‐immobilization process of the amylases, ii) required co‐immobilization time, iii) fermentation temperature and initial pH of starch, and iv) CB as single or double freeze‐dried are studied. The utilization of double freeze‐dried CB exhibits fermentation efficiency 89.9% and ethanol yield 0.51 g ethanol g−1 starch while the single freeze‐dried CB 81.1% and 0.46 g ethanol g−1 starch, respectively. In the case of double freeze‐dried CB, the fermentation efficiency decreases by only 27.1% in two‐recycling batches, while in the single freeze‐dried one decreases by 51.3%. The application of double freeze‐dried CB can be used for: i) the eco‐friendly biosynthesis of value‐added bioproducts; ii) the promising option for fuel‐grade bioethanol through starchy wastes or foodstuff starchy residues treatment, and iii) the implementation of industrialization. Finally, to simulate an industrial process of one‐step SSF of starch by applying a CB model, a technoeconomic analysis is evaluated, where using BNCs makes the bioprocess cost‐effective and environmentally favorable, simultaneously.

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Section: Scientific and Technological Consideration Of Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bacterial Nanocellulose‐Based Composite Biocatalysts for Starch‐to‐Bioethanol Valorization under Simultaneous Saccharification and Fermentation

Drosos,

Dima,

Kandylis

et al. 2023

Starch Stärke

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“…The strain of Aspergillus awamori 2B.361 U2/1 was grown as described in previous study (Drosos et al ., 2021).…”

Section: Methodsmentioning

confidence: 99%

“…For A. awamori , a solution of 0.001% v/v Tween‐80 in distilled water was used to prepare spore suspension from fungal cultures grown on PDA plates at 30°C for 4 days. The fungal spore‐titre (spores/mL) was counted by hemocytometer (Drosos et al ., 2021). The culture growth was adjusted to 0.5 McFarland turbidity standard (aprox.…”

Section: Methodsmentioning

confidence: 99%

Isolation of antimicrobial compounds from aniseed and techno‐economic feasibility report for industrial‐scale application

Κουτίνας

2022

Int J of Food Sci Tech

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Summary This work evaluates the antimicrobial activity of the fractionated aqueous extract obtained from the aerial parts of aniseed. Fifteen fractions were collected by column chromatography using ethanol and water as mobile phases. All fractions were tested as potential antimicrobial agents against three common food‐spoilage microorganisms: a yeast (Saccharomyces cerevisiae), a mold (Aspergillus awamori) and a bacterium (Lactobacillus delbrueckii subsp. bulgaricus). Fraction 2 exhibited the highest antimicrobial activity in both types of susceptibility test, and therefore, it was further studied for the identification of bioactive compounds. GC‐MS analysis of Fraction 2 revealed the presence of threo‐Anethole glycol. Based on laboratory‐scale experiments, a process flowsheet for potential industrial‐scale application is proposed combined with a techno‐economic feasibility report. Fraction 2 with the detected compound threo‐Anethole glycol showed promising results encouraging the attempts for industrial‐scale application of antimicrobial compounds produced from aniseed.

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“…If a reaction can be completed using intercellular or extracellular enzymes synthesised by specific microbes, the process of biotransformation would be economical for the large-scale synthesis of specialty molecules of industrial importance, such as flavours and aromas for the food industry and optically pure compounds for the pharmaceutical industry [4,5]. Researchers have designed effective cell-factories, incorporating enzymes as well as actively growing whole microbial cells, together in one biocatalyst entity [6][7][8]. Such cell-factories have been employed to perform multi-step (usually 2 to 3) processes for the synthesis of products by biotransformation and at the same time utilizing industrial by-products, towards a contribution to bioeconomy [9,10].…”

Section: Hydrolase Enzymesmentioning

confidence: 99%

Sustainable Biosynthesis of Esterase Enzymes of Desired Characteristics of Catalysis for Pharmaceutical and Food Industry Employing Specific Strains of Microorganisms

Dahiya

Nigam

2022

Sustainability

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Reactions catalysed by sustainably produced enzymes can contribute to the bioeconomy supporting several industries. Low-value compounds can be transformed into added-value products or high-resolution chemicals could be prepared in reactions catalysed by biocatalyst esterase enzymes. These enzymes can be synthesised by purposely isolated or genetically modified strains of microorganisms. Enzymes belonging to the hydrolase family catalyse the formation and hydrolysis of ester bonds to produce the desired esterified molecule. The synthesis of homo-chiral compounds can be accomplished either by chemical or biocatalytic processes, the latter being preferred with the use of microbial esterases. For varied applications, esterases with high stability and retained activity at lower and higher temperatures have been produced with strains isolated from extreme environments. For sustainable production of enzymes, higher productivity has been achieved by employing fast-growing Escherichia coli after incorporating plasmids of required characteristics from specific isolates. This is a review of the isolated and engineered strains used in the biosynthesis of esterase of the desired property, with the objective of a sustainable supply of enzymes, to produce products of industrial importance contributing to the economy.

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Consolidated bioprocessing of starch based on a bilayer cell factory without genetic modification of yeast

Cited by 10 publications

References 23 publications

Bacterial Nanocellulose‐Based Composite Biocatalysts for Starch‐to‐Bioethanol Valorization under Simultaneous Saccharification and Fermentation

Bacterial Nanocellulose‐Based Composite Biocatalysts for Starch‐to‐Bioethanol Valorization under Simultaneous Saccharification and Fermentation

Isolation of antimicrobial compounds from aniseed and techno‐economic feasibility report for industrial‐scale application

Sustainable Biosynthesis of Esterase Enzymes of Desired Characteristics of Catalysis for Pharmaceutical and Food Industry Employing Specific Strains of Microorganisms

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