Consistent view of electrolytes in aqueous two-phase systems

Pfennig, Andreas; Schwerin, A. v.; Gaube, Johann

doi:10.1016/s0378-4347(97)00593-8

Cited by 50 publications

(36 citation statements)

References 7 publications

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“…It was mainly a process in which the exposed groups of proteins come into contact with the phase components. It has been demonstrated that electrostatic potential difference between coexisting phases is a common property at interfaces even though the phases are electrically neutral (Pfennig et al 1998) and in this particular case the uneven distribution of Bmim + and salts generated a difference in electrical potential between the Table 1 Effect of concentration of [Bmim]BF 4 in ATPS on purification of wheat-esterase (x ± SD, n=3)…”

Section: Effect Of Concentration Of [Bmim]bfmentioning

confidence: 99%

Extraction and purification of wheat-esterase using aqueous two-phase systems of ionic liquid and salt

et al. 2014

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To explore a new and simple rapid extraction and purification technique for wheat-esterase, an ionic liquids (ILs)-based aqueous two-phase system (ATPS) was developed for the purification of wheat-esterase from wheat extracts. Effects of various process parameters such as the concentrations of [Bmim]BF 4 , the types and concentrations of phaseforming salt, the system pH and the temperature on partitioning of wheat-esterase were evaluated. The obtained data indicated that wheat-esterase was preferentially partitioned into the ILs-rich phase and the ATPS composed of 20 % [Bmim]BF 4 (w/w) and 25 % (w/w) NaH 2 PO 4 (pH= 4.8) showed good selectivity on wheat-esterase. Under the optimum conditions, wheat-esterase was purified with an acceptable yield (88.93 %), but produced wheat-esterase was 4.23 times as pure. It was obvious that temperature shows little influence on the purification between 10 and 50°C. Sephadex G-150FF revealed that the band intensity of contaminating proteins in ATPS fraction almost disappeared. Therefore, ILsbased ATPS was an effective method for partitioning and recovery of wheat-esterase from wheat crude extracts.

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Section: Effect Of Concentration Of [Bmim]bfmentioning

confidence: 99%

Extraction and purification of wheat-esterase using aqueous two-phase systems of ionic liquid and salt

et al. 2014

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“…Partitioning in aqueous two-phase systems is mainly a process in which the exposed groups of proteins come into contact with the phase components, and is therefore a surface-dependent phenomenon, depending strongly on the surface characteristics of the proteins. It has been demonstrated that electrostatic potential difference between coexisting phases is a common property at interfaces even though the phases are electrically neutral, [31] and in this particular case the uneven distribution of Bmim + and K 2 HPO 4 generates a difference in electrical potential between the phases. [32][33][34] The dominant components of proteins found in human urine in this study include albumin and transferrin, as described below, with isoelectric points at pH 4.7-5.2, and hence negatively charged in basic media.…”

mentioning

confidence: 99%

Extraction of Proteins from Biological Fluids by Use of an Ionic Liquid/Aqueous Two‐Phase System

2007

Chemistry A European J

287

178

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An ionic liquid/aqueous two-phase system based on the hydrophilic ionic liquid 1-butyl-3-methylimidazolium chloride (BmimCl) and K(2)HPO(4) has been employed for direct extraction of proteins from human body fluids for the first time. Proteins present at low levels were quantitatively extracted into the BmimCl-rich upper phase with a distribution ratio of about 10 between the upper and lower phase and an enrichment factor of 5. Addition of an appropriate amount of K(2)HPO(4) to the separated upper phase results in a further phase separation, giving rise to an improved enrichment factor of 20. FTIR and UV spectroscopy demonstrated that no chemical (bonding) interactions between the ionic liquid and the protein functional groups were identifiable, while no alterations of the natural properties of the proteins were observed. The partitioning of proteins in the two-phase system was assumed to have been facilitated by the electrostatic potential difference between the coexisting phases, as well as by salting out effects. The system could be applied successfully for the quantification of proteins in human urine after on-line phase separation in a flow system. The use of an ionic liquid, as a green solvent, offers clear advantages over traditional liquid-liquid extractions, in which the use of toxic organic solvents is unavoidable.

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“…Electroneutrality must be maintained in each phase. The electrochemical driving force in partitioning has been explained by the formation of an electrostatic potential difference over the interface [24][25][26][27]. This potential difference is created by the different species of the ions in the two phases.…”

Section: Effect Of Salts Species On the Partition Of Biomoleculesmentioning

confidence: 99%

Synthesis of a Novel pH-Sensitive Methacrylate Amphiphilic Polymer and Its Primary Application in Aqueous Two-phase Systems

Qin

Cao

2008

Appl Biochem Biotechnol

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In this study, a novel pH-sensitive and reversible water-soluble polymer(P(ABC)) forming aqueous two-phase systems(ATPS) was synthesized by using 2-(dimethylamino)ethyl methacrylate, t-butyl methacrylate, and methyl methacrylate as monomers and 2,2'-azo-bis-isobutyronitrile as initiator. The P(ABC) could be recovered by adjusting isoelectric point (PI) to 8.4, and recovery at PI could reach 95%. ATPS was formed by 5% (w/w) P(ABC) and 10% (w/w) PEG20000. The partition coefficient K of lysozyme was 6.8, and the partition coefficient K of bovine serum albumin could reach 12.5 in the ATPS.

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Consistent view of electrolytes in aqueous two-phase systems

Cited by 50 publications

References 7 publications

Extraction and purification of wheat-esterase using aqueous two-phase systems of ionic liquid and salt

Extraction and purification of wheat-esterase using aqueous two-phase systems of ionic liquid and salt

Extraction of Proteins from Biological Fluids by Use of an Ionic Liquid/Aqueous Two‐Phase System

Synthesis of a Novel pH-Sensitive Methacrylate Amphiphilic Polymer and Its Primary Application in Aqueous Two-phase Systems

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