Consistency of morphological endpoints used to assess developmental timing in zebrafish (Danio rerio) across a temperature gradient

A molecular method for assessing the effects of potential contaminants on the rate of zebrafish (Danio rerio) development

“…This comparison revealed that the 1.5‐h delay in the expression of sox2 widened to a 5‐h delay for tnnt1 , and the overall rate was slowed to 73% of that at 28.5 °C. These results were remarkably similar to those previously reported using morphological milestones (Figure ).…”

“…Open circles indicate mean onset of expression of sox2, shh, and tnnt1 at 24.5 °C ( n = 3). Solid lines indicate developmental rate at 28.5 °C and 24.5 °C as measured by Beasley et al .…”

Using zebrafish in systems toxicology for developmental toxicity testing

“…Zebrafish can tolerate a temperature range of 24.5 to 28.5°C (Beasley et al. ). However, the growth speed of zebrafish embryos varies according to temperature (Kimmel et al.…”

“…When zebrafish embryos are raised at 24.5 to 28.5°C, they reach sphere stage at 4.1 or 3.8 hpf, and shield stage at 5.9 or 5.4 hpf (Beasley et al. ). These findings suggest that chemical exposure at a given time‐point may have different effects on embryonic development, depending on the ambient temperature.…”

From cutting edge to guideline: A first step in harmonization of the zebrafish embryotoxicity test (ZET) by describing the most optimal test conditions and morphology scoring system

“…A temperature of 28 or 28.5 • C is used by several other research groups [2,[13][14][15]22]. The decision on optimal temperature is one of the most critical, as temperature has a direct impact on the development of the ZF embryo and its capacity to cover its developmental milestones, as development of ZF slows at lower temperatures [23]. In addition to its effect on ZF development rate, a higher temperature could also induce evaporation which could have a direct impact on the internal well concentration, and might therefore have a major impact on the study outcome for volatile test compounds.…”